ID F4SX43_ECOLX Unreviewed; 715 AA.
AC F4SX43;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=ECIG_00607 {ECO:0000313|EMBL:EGI17102.1};
OS Escherichia coli M605.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=656417 {ECO:0000313|EMBL:EGI17102.1, ECO:0000313|Proteomes:UP000004710};
RN [1] {ECO:0000313|EMBL:EGI17102.1, ECO:0000313|Proteomes:UP000004710}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M605 {ECO:0000313|EMBL:EGI17102.1,
RC ECO:0000313|Proteomes:UP000004710};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Feldgarden M., Gordon D.M., Johnson J.R., Johnston B.D., Young S., Zeng Q.,
RA Koehrsen M., Alvarado L., Berlin A.M., Borenstein D., Chapman S.B.,
RA Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E.R., Heiman D.I., Hepburn T.A., Howarth C., Jen D.,
RA Larson L., Lewis B., Mehta T., Park D., Pearson M., Richards J.,
RA Roberts A., Saif S., Shea T.D., Shenoy N., Sisk P., Stolte C., Sykes S.N.,
RA Walk T., White J., Yandava C., Haas B., Henn M.R., Nusbaum C., Birren B.;
RT "The Genome Sequence of Escherichia coli M605.";
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; GL883901; EGI17102.1; -; Genomic_DNA.
DR AlphaFoldDB; F4SX43; -.
DR HOGENOM; CLU_006494_0_1_6; -.
DR Proteomes; UP000004710; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR022161; Helicase_IV_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF63; DNA HELICASE IV; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12462; Helicase_IV_N; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 226..536
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 247..254
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 715 AA; 81478 MW; EFF674E4C3E135D4 CRC64;
MGGNCNVFLG WSTFSLTVTL IRNNFVWIYR VMELKATTLG KRLAQHPYDR AVILNAGIKV
SGDRHEYLIP FNQLLAIHCK RGLVWGELEF VLPDEKVVRL HGTEWGETQR FYHHLDAHWR
RWSGEMSEIA SGVLRQQLDL IATRTGENKW LTREQTSGVQ QQIRQALSAL PLPVNRLEEF
DNCREAWRKC QAWLIDIEGA RLQHNQAYTE AMLTEYADFF RQVESSPLNP AQARAVVNGE
HSLLVLAGAG SGKTSVLVAR AGWLLARGEA SPEQILLLAF GRKAAEEMDE RIRERLHTED
ITARTFHALA LHIIQQGSKK VPIVSKLEND TAARHELFIA EWRKQCSEKK AQAKGWRQWL
TEEMQWSVPE GNFWDDEKLQ RRLASRLDRW VSLMRMHGGA QAEMIASAPE EIRDLFSKRI
KLMAPLLKAW KGALKAENAV DFSGLIHQAI VILEKGRFIS PWKHILVDEF QDISPQRAAL
LAALRKQNSQ TTLFAVGDDW QAIYRFSGAQ MSLTTAFHEN FGEGDRCDLD TTYRFNSRIG
EVANRFIQQN PGQLKKPLNS LTNGDKKAVT LLDEGQLDAL LDKLSGYAKP EERILILARY
HHMRPASLEK AATRWPKLQI DFMTIHASKG QQADYVIIVG LQEGSDGFPA AARESIMEEA
LLPPVEDFPD AEERRLMYVA LTRARHRVWA LFNKENPSPF VEILKNLDVP VARKP
//
