ID F5LRC9_9BACL Unreviewed; 250 AA.
AC F5LRC9;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE SubName: Full=Butirosin biosynthesis radical SAM-dependent enzyme BtrN {ECO:0000313|EMBL:EGL15137.1};
GN Name=btrN {ECO:0000313|EMBL:EGL15137.1};
GN ORFNames=HMPREF9413_2348 {ECO:0000313|EMBL:EGL15137.1};
OS Paenibacillus sp. HGF7.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=944559 {ECO:0000313|EMBL:EGL15137.1, ECO:0000313|Proteomes:UP000003445};
RN [1] {ECO:0000313|EMBL:EGL15137.1, ECO:0000313|Proteomes:UP000003445}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HGF7 {ECO:0000313|EMBL:EGL15137.1,
RC ECO:0000313|Proteomes:UP000003445};
RA Durkin A.S., Madupu R., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGL15137.1}.
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DR EMBL; AFDH01000125; EGL15137.1; -; Genomic_DNA.
DR RefSeq; WP_009676482.1; NZ_AFDH01000125.1.
DR AlphaFoldDB; F5LRC9; -.
DR OrthoDB; 9805809at2; -.
DR Proteomes; UP000003445; Unassembled WGS sequence.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21129; SPASM_BtrN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR034386; BtrN-like.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR PANTHER; PTHR43787:SF10; COFACTOR MODIFYING PROTEIN-RELATED; 1.
DR PANTHER; PTHR43787; FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR SFLD; SFLDF00424; 2-deoxy-scyllo-inosamine_dehyd; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000003445};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 13..119
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|Pfam:PF04055"
FT DOMAIN 169..236
FT /note="4Fe4S-binding SPASM"
FT /evidence="ECO:0000259|Pfam:PF13186"
SQ SEQUENCE 250 AA; 28471 MW; 1FCBD9C3120922B1 CRC64;
MDKLFSMIEV EVNSQCNRTC WYCPNSVSQR KENGEMDPAL YKTLMEQLSS LDFAGRISFH
FYGEPLLCKN LDLFVGMTSE YIPRARPIIY TNGDFLTEKR LQTLTELGIQ KFIVTQHAGA
KHKFRGVYDQ LAGADKEKVV YLDHSDLILS NRGGILDNIP QASKANMSCM VPSNLAVVTV
LGNVLPCFED FNQKMVMGNI SEHHISDIWN NDKFTSFRKM LKEGHRGKSD LCKNCNNVSV
QTEDQYDYVL
//
