ID F5RIQ2_9FIRM Unreviewed; 433 AA.
AC F5RIQ2;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 24-JAN-2024, entry version 44.
DE SubName: Full=tRNA-I(6)A37 thiotransferase enzyme MiaB {ECO:0000313|EMBL:EGK62484.1};
DE EC=1.8.-.- {ECO:0000313|EMBL:EGK62484.1};
GN ORFNames=HMPREF9081_0231 {ECO:0000313|EMBL:EGK62484.1};
OS Centipeda periodontii DSM 2778.
OC Bacteria; Bacillota; Negativicutes; Selenomonadales; Selenomonadaceae;
OC Centipeda.
OX NCBI_TaxID=888060 {ECO:0000313|EMBL:EGK62484.1, ECO:0000313|Proteomes:UP000004067};
RN [1] {ECO:0000313|EMBL:EGK62484.1, ECO:0000313|Proteomes:UP000004067}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2778 {ECO:0000313|EMBL:EGK62484.1,
RC ECO:0000313|Proteomes:UP000004067};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGK62484.1}.
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DR EMBL; AFHQ01000004; EGK62484.1; -; Genomic_DNA.
DR RefSeq; WP_006305041.1; NZ_GL892076.1.
DR AlphaFoldDB; F5RIQ2; -.
DR STRING; 888060.HMPREF9081_0231; -.
DR eggNOG; COG0621; Bacteria.
DR HOGENOM; CLU_018697_1_0_9; -.
DR Proteomes; UP000004067; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035596; F:methylthiotransferase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR006467; MiaB-like_bact.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR01579; MiaB-like-C; 1.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR11918; RADICAL SAM PROTEINS; 1.
DR PANTHER; PTHR11918:SF45; THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01061; methylthiotransferase; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:EGK62484.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGK62484.1}.
FT DOMAIN 1..113
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 139..369
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 433 AA; 47825 MW; 9D1AEC01DE6B39D0 CRC64;
MRAAFMTLGC KVNQFETETM EGLFRARGYE VVPFDAAADV YVINTCSVTH LSDRKSRQLI
RRAARTNPSA CIAVTGCYAQ VAPEEIRALA GVRVVIGTKE RARIVDYVEA SLRENEGIAG
TITDIMQARV FEDIPLHSLP HRTRAFLKIE DGCQNFCTFC IIPYARGPVK SRALSAVARE
MRLLTEAGFH EVVLTGIHLG AYGIDLTDRP TLADACRTAL AEEKLRRLRL GSLESVELSA
DLLALMRTEP RFAAHLHLPL QAGSDNVLRA MNRHYDTAAF AQLIAEVRTA VPGVALSTDI
IVGFPGETEE DFATGMEFVR AMGFARMHVF PYSARKGTPA ARRADQVPPA VRKKRAARMQ
ALAEEMAEQY HRSQLGSVAD VLFETTEAGV TDGLTETYVR VYTDAPVQRG EILPMRLTHL
YRDGVWGEPA RAL
//
