ID F5XDR7_MICPN Unreviewed; 405 AA.
AC F5XDR7;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044150};
DE EC=1.5.3.24 {ECO:0000256|ARBA:ARBA00044044};
DE AltName: Full=Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta {ECO:0000256|ARBA:ARBA00044216};
DE AltName: Full=Tetrameric sarcosine oxidase subunit beta {ECO:0000256|ARBA:ARBA00044295};
GN Name=soxB {ECO:0000313|EMBL:BAK35090.1};
GN OrderedLocusNames=MLP_20760 {ECO:0000313|EMBL:BAK35090.1};
OS Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC
OS 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1).
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Propionibacteriaceae; Microlunatus.
OX NCBI_TaxID=1032480 {ECO:0000313|EMBL:BAK35090.1, ECO:0000313|Proteomes:UP000007947};
RN [1] {ECO:0000313|EMBL:BAK35090.1, ECO:0000313|Proteomes:UP000007947}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414
RC / VKM Ac-1990 / NM-1 {ECO:0000313|Proteomes:UP000007947};
RA Hosoyama A., Sasaki K., Harada T., Igarashi R., Kawakoshi A., Sasagawa M.,
RA Fukada J., Nakamura S., Katano Y., Hanada S., Kamagata Y., Nakamura N.,
RA Yamazaki S., Fujita N.;
RT "Whole genome sequence of Microlunatus phosphovorus NM-1.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2;
CC Xref=Rhea:RHEA:70455, ChEBI:CHEBI:15379, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:57305, ChEBI:CHEBI:57433,
CC ChEBI:CHEBI:57453; EC=1.5.3.24;
CC Evidence={ECO:0000256|ARBA:ARBA00043745};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; Evidence={ECO:0000256|ARBA:ARBA00043809};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the SoxB family.
CC {ECO:0000256|ARBA:ARBA00043973}.
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DR EMBL; AP012204; BAK35090.1; -; Genomic_DNA.
DR RefSeq; WP_013862962.1; NC_015635.1.
DR AlphaFoldDB; F5XDR7; -.
DR STRING; 1032480.MLP_20760; -.
DR KEGG; mph:MLP_20760; -.
DR eggNOG; COG0665; Bacteria.
DR HOGENOM; CLU_007884_4_1_11; -.
DR OrthoDB; 9805852at2; -.
DR Proteomes; UP000007947; Chromosome.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR006278; SoxB.
DR NCBIfam; TIGR01373; soxB; 1.
DR PANTHER; PTHR13847:SF287; FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR13847; SARCOSINE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022643};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Oxidoreductase {ECO:0000313|EMBL:BAK35090.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007947}.
FT DOMAIN 24..68
FT /note="Rhodanese"
FT /evidence="ECO:0000259|PROSITE:PS50206"
SQ SEQUENCE 405 AA; 43567 MW; 9303AECC96985352 CRC64;
MADALPEHPD FLWRNPDPKP AYDVVIVGAG GHGLATAYYL AKNHGITNVA VLERGWLAGG
NMARNTAIIR SNYLWDETSA IYEHSMKLWE GLPTELDYDF LFSQRGVMNL AHTLQDVRDS
VRRVSANQLN GIDAEWLDPQ QVKDLCPIIN ISGNIRYPVL GATYQPRGGI AKHDHVAWAF
ARQADALGVD LIQNCEVTGF VKDGNRVVGV ETSRGRIAAG RVGLAAAGHS SVLAKLAGFD
LPIQSHPLQA LVSELHQPVH PTVVMSNHVH VYVSQAHKGE LVMGAGIDAY NGYGQRGGFH
VIEHQMAAAV ELFPIFARAH LLRTWGGIVD VTLDASPIVG KAPVDDLYVN CGWGTGGFKG
TPGSGWTFAH TIATGEPHPL NAAFSLDRFV SGALIDEHGA AGVAH
//
