ID F6BBV9_METIK Unreviewed; 699 AA.
AC F6BBV9;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000256|HAMAP-Rule:MF_00442};
DE EC=5.6.2.4 {ECO:0000256|HAMAP-Rule:MF_00442};
GN Name=hel308 {ECO:0000256|HAMAP-Rule:MF_00442};
GN OrderedLocusNames=Metig_1707 {ECO:0000313|EMBL:AEF97239.1};
OS Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanotorris.
OX NCBI_TaxID=880724 {ECO:0000313|Proteomes:UP000009227};
RN [1] {ECO:0000313|EMBL:AEF97239.1, ECO:0000313|Proteomes:UP000009227}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5666 / JCM 11834 / Kol 5
RC {ECO:0000313|Proteomes:UP000009227};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Chertkov O., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Pagani I., Sieprawska-Lupa M.,
RA Whitman W., Woyke T.;
RT "Complete sequence of Methanotorris igneus Kol 5.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000256|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618, ECO:0000256|HAMAP-
CC Rule:MF_00442};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617, ECO:0000256|HAMAP-
CC Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00442}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00442}.
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DR EMBL; CP002737; AEF97239.1; -; Genomic_DNA.
DR RefSeq; WP_013799828.1; NC_015562.1.
DR AlphaFoldDB; F6BBV9; -.
DR STRING; 880724.Metig_1707; -.
DR GeneID; 10644580; -.
DR KEGG; mig:Metig_1707; -.
DR HOGENOM; CLU_006553_3_0_2; -.
DR OrthoDB; 371946at2157; -.
DR Proteomes; UP000009227; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd18028; DEXHc_archSki2; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR Gene3D; 1.10.3380.30; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR048772; Hel308-like_dom4.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR47961:SF10; ATP-DEPENDENT DNA HELICASE HEL308; 1.
DR PANTHER; PTHR47961; DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21280; Helicase_dom4_arc; 1.
DR Pfam; PF14520; HHH_5; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF158702; Sec63 N-terminal domain-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00442}; DNA damage {ECO:0000256|HAMAP-Rule:MF_00442};
KW DNA repair {ECO:0000256|HAMAP-Rule:MF_00442};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_00442};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_00442};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00442};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_00442};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00442}; Reference proteome {ECO:0000313|Proteomes:UP000009227}.
FT DOMAIN 29..199
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 232..430
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT BINDING 23
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00442"
SQ SEQUENCE 699 AA; 80154 MW; 3EAFA827015AFC68 CRC64;
MQKYSHVFEV LKENGIKELR PPQKKVIEKG LLNKEKNFLI CIPTASGKTL IGEMALINHL
LDENKTPTNK KGLFIVPLKA LASEKYEEFK RKYEKYGLKV ALSIGDYDEK EDLSSYNIII
TTAEKLDSLM RHEIDWLNYV SVAIVDEIHM INDEKRGGTL EVLLTKLKNL DVQIIGLSAT
IGNPEELAEW LNAELIIDNW RPVKLRKGIF FQNKIMYLNG ACKELPNFSN NPMLNLVLDC
VKEGGCCLVF CNSKNGAVSE AKKLNLKKYL SNSEKYELQK LKEEILSILD PPTETCKTLA
ECLEKGVAFH HAGLTYEHRK IVEEGFRNKL IKVICCTPTL SAGINIPCRR AIVRDLMRFS
NGRMKPIPIM EIHQCIGRAG RPGLDPYGEG IIFVKNERDL ERAEQYLEGK PEYIYSKLSN
QAVLRTQLLG MIATREIENE FDLISFIKNT FYAHQYGNLG GVLRNIKEVI NFLEENDFIA
DYFPTKLGKR VSELYIDPLS AKIIIDGLKE MGNVDNEELY YLYLISKTLE MMPLLRVNSF
EELDLILEME EAGIYDRTYD DLAAFKNAKM LYDWINEVPE DEILKKYKIE PGILRYKVEQ
AKWMIYSTKE IAKLLNRNID TLSKLEIRLE YGAKEDIIEL LKIKYVGRAR ARKLYDAGIR
SVEDIINNPK KVASLLGEKI AKKILGELGM KFGQQTLQI
//
