ID F6FZ16_RALS8 Unreviewed; 694 AA.
AC F6FZ16;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=DNA-3-methyladenine glycosylase II {ECO:0000256|ARBA:ARBA00012000};
DE EC=3.2.2.21 {ECO:0000256|ARBA:ARBA00012000};
GN Name=ada {ECO:0000313|EMBL:AEG68250.1};
GN OrderedLocusNames=RSPO_c00949 {ECO:0000313|EMBL:AEG68250.1};
OS Ralstonia solanacearum (strain Po82).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=1031711 {ECO:0000313|EMBL:AEG68250.1, ECO:0000313|Proteomes:UP000007953};
RN [1] {ECO:0000313|EMBL:AEG68250.1, ECO:0000313|Proteomes:UP000007953}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Po82 {ECO:0000313|EMBL:AEG68250.1,
RC ECO:0000313|Proteomes:UP000007953};
RX PubMed=21685279; DOI=10.1128/JB.05384-11;
RA Xu J., Zheng H.J., Liu L., Pan Z.C., Prior P., Tang B., Xu J.S., Zhang H.,
RA Tian Q., Zhang L.Q., Feng J.;
RT "Complete genome sequence of the plant pathogen Ralstonia solanacearum
RT strain Po82.";
RL J. Bacteriol. 193:4261-4262(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-
CC methylguanine, 7-methylguanine and 7-methyladenine.; EC=3.2.2.21;
CC Evidence={ECO:0000256|ARBA:ARBA00000086};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR EMBL; CP002819; AEG68250.1; -; Genomic_DNA.
DR AlphaFoldDB; F6FZ16; -.
DR KEGG; rsn:RSPO_c00949; -.
DR PATRIC; fig|1031711.3.peg.930; -.
DR eggNOG; COG0122; Bacteria.
DR eggNOG; COG2169; Bacteria.
DR HOGENOM; CLU_000445_72_6_4; -.
DR Proteomes; UP000007953; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00056; ENDO3c; 1.
DR Gene3D; 3.40.10.10; DNA Methylphosphotriester Repair Domain; 1.
DR Gene3D; 3.30.310.20; DNA-3-methyladenine glycosylase AlkA, N-terminal domain; 1.
DR Gene3D; 1.10.1670.10; Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal); 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 2.
DR InterPro; IPR035451; Ada-like_dom_sf.
DR InterPro; IPR004026; Ada_DNA_repair_Zn-bd.
DR InterPro; IPR010316; AlkA_N.
DR InterPro; IPR037046; AlkA_N_sf.
DR InterPro; IPR011257; DNA_glycosylase.
DR InterPro; IPR003265; HhH-GPD_domain.
DR InterPro; IPR023170; HhH_base_excis_C.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR PANTHER; PTHR43003; DNA-3-METHYLADENINE GLYCOSYLASE; 1.
DR PANTHER; PTHR43003:SF13; DNA-3-METHYLADENINE GLYCOSYLASE 2; 1.
DR Pfam; PF02805; Ada_Zn_binding; 1.
DR Pfam; PF06029; AlkA_N; 1.
DR Pfam; PF12833; HTH_18; 1.
DR SMART; SM01009; AlkA_N; 1.
DR SMART; SM00478; ENDO3c; 1.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF57884; Ada DNA repair protein, N-terminal domain (N-Ada 10); 1.
DR SUPFAM; SSF48150; DNA-glycosylase; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 2.
DR SUPFAM; SSF55945; TATA-box binding protein-like; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|ARBA:ARBA00023159};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AEG68250.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AEG68250.1};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 288..386
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000259|PROSITE:PS01124"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 694 AA; 76108 MW; 68ACC34947043940 CRC64;
MRRAQPRRRR RHVHQRTARA AARGRQPPRR FARHQERAQH IGLEHRAQPL HAHVGHILGR
RNDACIVDHT VQRAEAVLRR IEQRRHILLA RHIALHRQRL PAGLLDEAER LVGGVPVTAI
LDRHGPPAPR AQNRRGAPDA PAAAGDDDDP GHASLPKWCR TRIVPARPRP EGGGRPREMS
ESGERGPRMG TTLGRESTAP TMHLDHDACY HAVQSRDRRF DGWFFVGVTS TGVYCRPICA
VRTPLQKNCR FFATAAAAEK AGFRPCLRCR PELAPGHSLA EMSSSLARAA ARMIDEGFLQ
QHDLAALAAA VGVTDRHLRR IFRTEFDVSP IEYAQTQRLL LAKQLLTDTD MPVGDIAYAA
GFGSVRRLNS GFAEHYGFAP TRLRTRAAPV PRSDDGATLM LGYRPPFAWH ALLDFLRARA
VDGVETVDAH SYARTIALDY AGRRHAGWLH ARDVPQRHAV ALTLSASLMQ AMPPVLARVR
RLFDLDCRPD LVDAHLGTLA AGTPGLRVPG AVDGFEIAVR AIAGQVVSLA QARRILARLV
VAHGTPLTQP RAGLPMAFPS ATALAGSDPL TLSRETGLQA SRAAAVVEIA RAVDAGRLRL
EPLVPLVPLA PTLAALQALP GIGEWTAQYI AMRALGWPNA FPLGDYVLRK RLADHDDALP
TRHAMATRAE GWAPWRAYAA MHLWHHDVAL APTP
//
