ID F6PT04_HORSE Unreviewed; 1130 AA.
AC F6PT04;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 4.
DT 27-MAR-2024, entry version 79.
DE SubName: Full=MCF.2 cell line derived transforming sequence like {ECO:0000313|Ensembl:ENSECAP00000015005.4};
GN Name=MCF2L {ECO:0000313|VGNC:VGNC:20028};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000015005.4, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000015005.4, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000015005.4,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000015005.4}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000015005.4};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR AlphaFoldDB; F6PT04; -.
DR Ensembl; ENSECAT00000018395.4; ENSECAP00000015005.4; ENSECAG00000016662.4.
DR VGNC; VGNC:20028; MCF2L.
DR GeneTree; ENSGT00940000157874; -.
DR HOGENOM; CLU_007130_1_0_1; -.
DR TreeFam; TF318080; -.
DR Proteomes; UP000002281; Chromosome 17.
DR Bgee; ENSECAG00000016662; Expressed in retina and 21 other cell types or tissues.
DR ExpressionAtlas; F6PT04; baseline.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0035091; F:phosphatidylinositol binding; IEA:Ensembl.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd01227; PH_Dbs; 1.
DR CDD; cd00160; RhoGEF; 1.
DR CDD; cd00170; SEC14; 1.
DR CDD; cd11857; SH3_DBS; 1.
DR CDD; cd00176; SPEC; 1.
DR Gene3D; 1.20.58.60; -; 1.
DR Gene3D; 3.40.525.10; CRAL-TRIO lipid binding domain; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR035534; DBS_PH.
DR InterPro; IPR035532; DBS_SH3.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR22826:SF115; GUANINE NUCLEOTIDE EXCHANGE FACTOR DBS; 1.
DR PANTHER; PTHR22826; RHO GUANINE EXCHANGE FACTOR-RELATED; 1.
DR Pfam; PF13716; CRAL_TRIO_2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF00435; Spectrin; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00516; SEC14; 1.
DR SMART; SM00150; SPEC; 1.
DR SUPFAM; SSF52087; CRAL/TRIO domain; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF50044; SH3-domain; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 1.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 22..194
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000259|PROSITE:PS50191"
FT DOMAIN 601..781
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 799..915
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1056..1117
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT REGION 526..595
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 930..999
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1012..1056
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 575..595
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 930..951
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1012..1040
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1130 AA; 127772 MW; 776FC1022863B5C5 CRC64;
MRFWLRNEEM ALEEMVQRLN AVSARTDEIM HQDITPLCAA DIQDQLRKRF AYLSGGRGQD
GSPVITFPDY PAFSDVPDKE FQNVMTYLTS IPSLQDAGIG FILVIDRRQD KWTSVKASVL
RIAASFPANL QRVLVLRPTG FFQRTLSDLA FRFNRDDFKM KVPVTMLSSV PELHDYIDKS
QLTEDLGGTL DYCHTKWLCH RTAIESFAVM VKQTAQMLQS FGTELAETEL PNDVPSTSSV
LIAHTEKKHK AKENMRLALD EGRSILESIR EPLARSTEQS PNQDQLDNQT TVQRLLAQLN
ETEAAFDEFW AKHQQKLEQC LQLRHFEQDF REVKAALDTL AQKIVTFTDV GNSLAHVEHL
LKDLTSFEEK SSAAVERARA LSLEGEQLIG NKHYAVDSIR PKCHELQHLC DQFAAEVGRR
RGLLGKSLEL HSLLEASMKW CDEGIYLLAS QPVDKCQSQD GAEAALQEIE KFLETGAENK
IQELNKIYQD YELILTQDLR EHVQKVFQKQ ESMEEMFHRR QASLKKLAAK QTRPVQPVAP
RPEALAKSPC ASPGIRRGSE NSSCEGSAQR GPYRRAKSEM SEGRQGRGSS TGDEEESLAI
LRRHVMNELL DTERVYVEEL LCVLEGYAAE MDNPLMTHLI STGLQNKKDV LFGNMEEIYH
FHNRIFLREL ENYIDCPELV GRCFLERLEE FQMYEKYCQN KPRSESVWRQ CADCPFFQEC
QKKLDHKLSL DSYLLKPVQR ITKYQLLLKE MLKYSKSCEG AEDLQEALSS ILGILKAVND
SMHLIAITGY DGNLSDLGKL LMQGSFSVWT DHKKGHSKVK DLARFKPMQR HLFLHEKAVL
FCKKREENGE GYEKAPSYSY KQSLNMAAVG ITENVKGDVR KFEIWYNARE EVYIIQAPTP
EIKAAWVSEI RKLLTSQLQA FREASQHRVL EQSQSLPLPA TASASPSKGN TRNVKKLEER
KTDPLSLEGY VSSTSLPKAP EKGRDDTVTN STSESSALSK KRFTLQGFAN LKGQKGWSKT
SQPSEAPEDN DGWSSAEEPL NSSDAEEEGG VGPRKLVPGR FTVVADDEKG SPDTLVMRSG
DLVEVVQEGE DCLWYVRNLT SSKEGWVPAG SLSARLGKSS SAQCLSSSVW
//
