ID F7E3A6_HORSE Unreviewed; 484 AA.
AC F7E3A6;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 4.
DT 27-MAR-2024, entry version 63.
DE SubName: Full=Pseudouridine synthase 1 {ECO:0000313|Ensembl:ENSECAP00000005044.4};
GN Name=PUS1 {ECO:0000313|VGNC:VGNC:22047};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000005044.4, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000005044.4, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000005044.4,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000005044.4}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000005044.4};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR AlphaFoldDB; F7E3A6; -.
DR Ensembl; ENSECAT00000006984.4; ENSECAP00000005044.4; ENSECAG00000006965.4.
DR VGNC; VGNC:22047; PUS1.
DR GeneTree; ENSGT00950000183160; -.
DR HOGENOM; CLU_021971_3_0_1; -.
DR OMA; CDARTYT; -.
DR OrthoDB; 12038at2759; -.
DR TreeFam; TF314367; -.
DR Proteomes; UP000002281; Chromosome 8.
DR Bgee; ENSECAG00000006965; Expressed in trophoblast and 23 other cell types or tissues.
DR ExpressionAtlas; F7E3A6; baseline.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:Ensembl.
DR GO; GO:0002153; F:steroid receptor RNA activator RNA binding; IEA:Ensembl.
DR GO; GO:0000049; F:tRNA binding; IEA:Ensembl.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IEA:Ensembl.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:Ensembl.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 300..402
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 20..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 207
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 262
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 484 AA; 54050 MW; 8A57BA5C78D81F30 CRC64;
MKRAQKPGSL SCRLFCSLRE AAQQRSQPPR TPGVRAGKPR PAKPRPRQSP ARKQVCPRPA
AVRRALRFRL RPRGAARAQS AAPSPPMAGN VQAPAPVGAR LQQDRKARSG WQGGARVWEE
TEQQAKKLKS SGDEDGEQHR KLPKRKIVLL MAYSGKGYHG MQRNVGSSQF KTIEDDLVSA
LVRSGCIPEN HGEDMRKMSF QRCARTDKGV SAAGQVVSLK VWLIDDILEK INSHLPSHIR
ILGLKRVTGG FNSKNKCDAR TYFYMLPTFA FAHKDRDVQD ETYRLTAETL QSVNRLLACY
KGTHNFHNFT SQKGPREPSA QRYILDMYCE EPFVREGMEF AVIKVKGQSF MTHQIRKMVG
LVVAIVKGYA PESVLARSWG EAKVDVPKAP GLGLVLERVH FEKYNQRFGN DGLHEPLDWA
QEEAEVAAFK EQHIYPAIVS TERHERSMAQ WLSTLPVHDF SATAHAAAKV PSPLEGSEGD
GDSD
//
