ID F7E820_XENTR Unreviewed; 1543 AA.
AC F7E820;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 02-JUN-2021, sequence version 4.
DT 27-MAR-2024, entry version 82.
DE SubName: Full=Rap guanine nucleotide exchange factor 6 {ECO:0000313|Ensembl:ENSXETP00000018397, ECO:0000313|RefSeq:XP_017947623.1};
GN Name=rapgef6 {ECO:0000313|Ensembl:ENSXETP00000018397,
GN ECO:0000313|RefSeq:XP_017947623.1,
GN ECO:0000313|Xenbase:XB-GENE-981546};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364 {ECO:0000313|Ensembl:ENSXETP00000018397};
RN [1] {ECO:0000313|Ensembl:ENSXETP00000018397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nigerian {ECO:0000313|Ensembl:ENSXETP00000018397};
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2] {ECO:0000313|Ensembl:ENSXETP00000018397}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:XP_017947623.1}
RP IDENTIFICATION.
RC STRAIN=Nigerian {ECO:0000313|RefSeq:XP_017947623.1};
RC TISSUE=Liver and blood {ECO:0000313|RefSeq:XP_017947623.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
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DR RefSeq; XP_017947623.1; XM_018092134.2.
DR Ensembl; ENSXETT00000018397; ENSXETP00000018397; ENSXETG00000026511.
DR GeneID; 733941; -.
DR CTD; 51735; -.
DR Xenbase; XB-GENE-981546; rapgef6.
DR eggNOG; KOG3542; Eukaryota.
DR TreeFam; TF313184; -.
DR Proteomes; UP000008143; Chromosome 3.
DR Bgee; ENSXETG00000026511; Expressed in blastula and 16 other cell types or tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF4; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 6; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 2.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 2.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008143}.
FT DOMAIN 280..380
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 412..525
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 530..600
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 756..842
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 867..1094
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 179..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1081..1107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1163..1190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1271..1291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1448..1493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1081..1096
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1174..1190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1461..1475
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1476..1493
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1543 AA; 174074 MW; CFAA6EC8E6F20477 CRC64;
MSASIEPRFK EALRKKPKER TQEDLKTVYS YMHRMDILSH LREHQLRLMS ATARYERYSA
NQVLFCSESL ARCWYILLSG SVLVKNSMFL PPCSFGKQSG GKRGCDCIVL EPSEMIVVEN
TGENNDGILH RETSIRQSRR RYRKINQHGE RQTITDNGDA ASYVLLPTDL SKIHLTDSPH
PHVTHVPSSQ SGCSIASDSG SSSLSDIYQA TESEMGDVDL TGLPEAAVDS EDEEEDEDIE
RASDTMLTRD IVRECLEKEP TDRTDDDIEQ LLEFMHQLPA FVNMTMSVRR KLCSVMIFEV
VEQAGAIIIE DGQELDSWYV ILNGIVEISH SDGRMENLCM GNSFGINPSL ETQYMDGVVK
TKADDCQFVC IAQQDYFRIL NHVEKNTHKV KEKGEIVMVN EHRELDRSGT RKGHIVIQGT
PERLILHLIE EHSVVDPTYI EDFLLTYRTF LSSPLEVGDK LLEWFKDDSR RDKVTRVVLL
WVNNHFNDFE GDPAMTRFLE EFERNLENAK MKGHLRLLNI ACAAKAKLRQ VTLLKPSRES
PFYFNVVGGS DKGFGMFIES VEPGSKATEA GLKRGDQIME VNGQNFETIV YAKALEILKN
NTHLSITVKT NIFVFKELLN RIGQEKKNGV PHIPKIAEKK NNRYSIPDLP NDVEQMFPRD
RASKKMKANT VSGGRNRIRK ILDKTRFSIL PPKPFSDGHV IQSQDDSIVG TRQCRHSVAI
MPIPGTLSSS SPDLLQATTN ILDFSNPTAF GFYYIPDQVI RIFKADQQCC YIIIGKDTTA
KEVVIQALHE FSLTGSTDTY SLCEVSVSPE GVIKQRRLPD QLSKLADRIQ LNGRYYLKNN
METETLCSDE DAQELLRESL ISLLQLSTIE VATQLSMRDF ELFRNIEPTE YIDDLFKIDS
KTSAAHLKQF EEVINQETFW VATEILREPN HLKRMKIVKH FIKTALHCRE CRNFNSMFAI
ISGLNLAPVA RLRGTWEKLP SKYEKLLRDL QDLFDPSRNM AKYRNILSSQ SMQPPIIPLF
PVVKKDITFL HEGNDSKVDG LVNFEKLRMI AKEIRHVIRM TSANMDPAVM FRQRKKRWRS
LGSLSQGSTN SNMLDVQGGG HKKRARRSSF LNAKKLYEDA QMSRKVKQYI SNLSVEIDEE
KIQLMSLQCE PAYSTLAKNL SERRSAKSDL SPAPLRPSGQ TTKAHIHQQN RMSQVLQVPT
VNLYPMRKKA PTKEHLPCTS GSSQKISGLT DEVTVKAQLE DSVSLVSSLH SSPAASPQGS
PHKVVNISRS ENSSHMNLSG SSSSLTSDPN QKICSRRGYG IGYAHNPSAK SDNFSDSSNS
EISSRSSLVS NCSVDSMSLT LQDEKCVPHS HAESMGLSER KDVHPSSEYN QPCSSWLHAR
HPAVRNFIAC ASSEDIAHDH ISVEPVDSGR GSWTSCSSSS HDNFQTLQKG LDRLSTYRQS
HIDSPIAETE SANCSTDETC SEDCTKGKRH SNNSLELNES RQSWASSSSM SDTYETNYGT
IKRRVLDTEE KTEALGQQLD VFYKTVTSTT EKGLIEDEQM SAV
//
