ID F7LY75_9BACE Unreviewed; 670 AA.
AC F7LY75;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 24-JAN-2024, entry version 59.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0000256|ARBA:ARBA00012541};
DE EC=2.4.1.18 {ECO:0000256|ARBA:ARBA00012541};
GN ORFNames=HMPREF0127_00165 {ECO:0000313|EMBL:EGN06252.1};
OS Bacteroides sp. 1_1_30.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=457387 {ECO:0000313|EMBL:EGN06252.1, ECO:0000313|Proteomes:UP000004085};
RN [1] {ECO:0000313|EMBL:EGN06252.1, ECO:0000313|Proteomes:UP000004085}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1_1_30 {ECO:0000313|EMBL:EGN06252.1,
RC ECO:0000313|Proteomes:UP000004085};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Daigneault M., Strauss J.,
RA Allen-Vercoe E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Brown A.,
RA Chapman S.B., Chen Z., Dunbar C., Freedman E., Gearin G., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heiman D., Howarth C., Larson L., Lui A.,
RA MacDonald P.J.P., Mehta T., Montmayeur A., Murphy C., Neiman D.,
RA Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P.,
RA Stolte C., Sykes S., Yandava C., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Bacteroides sp. 1_1_30.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000256|ARBA:ARBA00000826};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000256|ARBA:ARBA00009000}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGN06252.1}.
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DR EMBL; ADCL01000004; EGN06252.1; -; Genomic_DNA.
DR RefSeq; WP_008640123.1; NZ_JAJKHN010000031.1.
DR AlphaFoldDB; F7LY75; -.
DR GeneID; 69480109; -.
DR PATRIC; fig|457387.3.peg.155; -.
DR HOGENOM; CLU_011131_2_2_10; -.
DR Proteomes; UP000004085; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:InterPro.
DR CDD; cd11321; AmyAc_bac_euk_BE; 1.
DR CDD; cd02854; E_set_GBE_euk_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 182..544
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 329
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
FT ACT_SITE 383
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
SQ SEQUENCE 670 AA; 77362 MW; 44651E9E55DF63A3 CRC64;
MEKTLNLIKN DPWLEPFAGA ITGRHQHVLD KEAELTNKGK QTLSDFASGY LYFGLHRTDK
GWTFREWAPN ATHIYMVGTF NNWEEKAAYK LKKLKNGNWE INLPADAIHH GDLYKLNVYW
EGGQGERIPA WATRVVQDEQ TKIFSAQVWA PEKPYKFKKK TFKPDTNPLL IYECHIGMAQ
REEKVGTYNE FREKILPRIA EEGYNCIQIM AIQEHPYYGS FGYHVSSFFA ASSRFGTPDE
LKALIDAAHE MGIAVIMDIV HSHAVKNEVE GLGNFAGDPN QYFYPGVRRE HPAWDSLCFD
YGKNEVIHFL LSNCKYWLEE YKFDGFRFDG VTSMLYYSHG LGEAFCNYGD YFNGHQDDNA
ICYLTLANEV IHQVNPKAIT IAEEVSGMPG LAAKIEDGGY GFDYRMAMNI PDYWIKTIKE
KIDEDWKPSS MFWEVTNRRQ DEKTISYAES HDQALVGDKT IIFRLIDADM YWHMQKGDEN
YVVNRGIALH KMIRLLTSST INGGYLNFMG NEFGHPEWID FPREGNGWSC KYARRQWDLV
DNKNLTYHYM GDFDKNMLKV LKSVKNFQTT PVQEIWHNDG DQVLAYGRKD LIFVFNFNPK
QSFTDYGFLV TPGAYEVILN TDDVAFGGNG LADDSVVHFT IADPLYSKEK KEWLKLYIPA
RTAVVLRKKK
//
