ID F7M810_9BACE Unreviewed; 435 AA.
AC F7M810;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444};
DE EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444};
DE AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444};
GN ORFNames=HMPREF0127_03596 {ECO:0000313|EMBL:EGM99377.1};
OS Bacteroides sp. 1_1_30.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=457387 {ECO:0000313|EMBL:EGM99377.1, ECO:0000313|Proteomes:UP000004085};
RN [1] {ECO:0000313|EMBL:EGM99377.1, ECO:0000313|Proteomes:UP000004085}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1_1_30 {ECO:0000313|EMBL:EGM99377.1,
RC ECO:0000313|Proteomes:UP000004085};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Daigneault M., Strauss J.,
RA Allen-Vercoe E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Brown A.,
RA Chapman S.B., Chen Z., Dunbar C., Freedman E., Gearin G., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heiman D., Howarth C., Larson L., Lui A.,
RA MacDonald P.J.P., Mehta T., Montmayeur A., Murphy C., Neiman D.,
RA Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P.,
RA Stolte C., Sykes S., Yandava C., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Bacteroides sp. 1_1_30.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to
CC phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-phenylacetate + ATP + CoA = AMP + diphosphate +
CC phenylacetyl-CoA; Xref=Rhea:RHEA:20956, ChEBI:CHEBI:18401,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57390, ChEBI:CHEBI:456215; EC=6.2.1.30;
CC Evidence={ECO:0000256|PIRNR:PIRNR006444};
CC -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family.
CC {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGM99377.1}.
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DR EMBL; ADCL01000069; EGM99377.1; -; Genomic_DNA.
DR RefSeq; WP_004313249.1; NZ_GL945099.1.
DR AlphaFoldDB; F7M810; -.
DR GeneID; 69479544; -.
DR PATRIC; fig|457387.3.peg.3661; -.
DR HOGENOM; CLU_035301_1_1_10; -.
DR UniPathway; UPA00930; -.
DR Proteomes; UP000004085; Unassembled WGS sequence.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0010124; P:phenylacetate catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd05913; PaaK; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR028154; AMP-dep_Lig_C.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR011880; PA_CoA_ligase.
DR PANTHER; PTHR43845; BLR5969 PROTEIN; 1.
DR PANTHER; PTHR43845:SF1; BLR5969 PROTEIN; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF14535; AMP-binding_C_2; 1.
DR PIRSF; PIRSF006444; PaaK; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR006444};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444}.
FT DOMAIN 90..287
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 337..432
FT /note="AMP-dependent ligase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14535"
SQ SEQUENCE 435 AA; 49056 MW; 2374B099D2BA2E07 CRC64;
MSTQYWEEEL ETMSREKLQE LQLQRLKKTI NIAANAPYYK EVFSKHGITA DSIQSLDDIR
KVPFTTKSDM RAHYPFGLVA GDMSNDGVRI HSSSGTTGNP TVIVHSQHDL DSWANLVARC
LYAVGIRKTD VFQNSSGYGM FTGGLGFQYG AERLGCLTVP AAAGNSKRQI KFINDFKTTA
LHAIPSYAIR LAEVFQEEGL DPKGTTLKTL VIGAEPHTDE QRRKIEKMLG VKAYNSFGMT
EMNGPGVAFE CQEQNGMHFW EDCYLVEIID PETGEPVPEG EIGELVLTTL DREMMPLIRY
RTRDLTRILP GKCPCGRTHI RIDRIKGRSD DMFIIKGVNI FPMQVEKILV QFPELGSNYL
ITLETVNNQD EMIVEVELSD LSTDNYIELE KIRKDITRQL KDEILVTPKV KLVKKGSLPQ
SEGKAVRVKD LRDNK
//
