ID F7PKA4_9EURY Unreviewed; 326 AA.
AC F7PKA4;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 24-JAN-2024, entry version 52.
DE RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE EC=1.5.98.2 {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Methylene-H(4)MPT reductase {ECO:0000256|HAMAP-Rule:MF_01091};
GN Name=mer {ECO:0000256|HAMAP-Rule:MF_01091,
GN ECO:0000313|EMBL:ERJ07626.1};
GN ORFNames=HLRTI_000379 {ECO:0000313|EMBL:ERJ07626.1}, HTIA_1288
GN {ECO:0000313|EMBL:CCQ33422.1};
OS Halorhabdus tiamatea SARL4B.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Haloarculaceae; Halorhabdus.
OX NCBI_TaxID=1033806 {ECO:0000313|EMBL:ERJ07626.1, ECO:0000313|Proteomes:UP000003861};
RN [1] {ECO:0000313|EMBL:ERJ07626.1, ECO:0000313|Proteomes:UP000003861}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SARL4B {ECO:0000313|EMBL:ERJ07626.1,
RC ECO:0000313|Proteomes:UP000003861};
RX PubMed=21705593; DOI=10.1128/JB.05462-11;
RA Antunes A., Alam I., Bajic V.B., Stingl U.;
RT "Genome sequence of Halorhabdus tiamatea, the first archaeon isolated from
RT a deep-sea anoxic brine lake.";
RL J. Bacteriol. 193:4553-4554(2011).
RN [2] {ECO:0000313|EMBL:ERJ07626.1, ECO:0000313|Proteomes:UP000003861}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SARL4B {ECO:0000313|EMBL:ERJ07626.1,
RC ECO:0000313|Proteomes:UP000003861};
RX PubMed=24324765;
RA Alam I., Antunes A., Kamau A.A., Ba Alawi W., Kalkatawi M., Stingl U.,
RA Bajic V.B.;
RT "INDIGO - INtegrated Data Warehouse of MIcrobial GenOmes with Examples from
RT the Red Sea Extremophiles.";
RL PLoS ONE 8:E82210-E82210(2013).
RN [3] {ECO:0000313|EMBL:CCQ33422.1, ECO:0000313|Proteomes:UP000015381}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SARL4B {ECO:0000313|Proteomes:UP000015381}, and Type strain:
RC SARL4B {ECO:0000313|EMBL:CCQ33422.1};
RX PubMed=24428220; DOI=10.1111/1462-2920.12393;
RA Werner J., Ferrer M., Michel G., Mann A.J., Huang S., Juarez S.,
RA Ciordia S., Albar J.P., Alcaide M., La Cono V., Yakimov M.M., Antunes A.,
RA Taborda M., Da Costa M.S., Amann R.I., Gloeckner F.O., Golyshina O.V.,
RA Golyshin P.N., Teeling H.;
RT "Halorhabdus tiamatea: proteogenomics and glycosidase activity measurements
RT identify the first cultivated euryarchaeon from a deep-sea anoxic brine
RT lake as potential polysaccharide degrader.";
RL Environ. Microbiol. 16:2525-2537(2014).
CC -!- FUNCTION: Catalyzes the oxidation of methyl-H(4)MPT to methylene-
CC H(4)MPT. {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01091};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- SIMILARITY: Belongs to the mer family. {ECO:0000256|HAMAP-
CC Rule:MF_01091}.
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DR EMBL; HF571520; CCQ33422.1; -; Genomic_DNA.
DR EMBL; AFNT02000002; ERJ07626.1; -; Genomic_DNA.
DR RefSeq; WP_008526292.1; NZ_AFNT02000002.1.
DR STRING; 1033806.HTIA_1288; -.
DR GeneID; 23800152; -.
DR KEGG; hti:HTIA_1288; -.
DR PATRIC; fig|1033806.12.peg.1281; -.
DR eggNOG; arCOG02410; Archaea.
DR HOGENOM; CLU_027853_5_3_2; -.
DR OrthoDB; 213164at2157; -.
DR Proteomes; UP000003861; Unassembled WGS sequence.
DR Proteomes; UP000015381; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR HAMAP; MF_01091; F420_mer; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019946; MeH4methanopterin_reductase.
DR PANTHER; PTHR43244; -; 1.
DR PANTHER; PTHR43244:SF1; 5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01091};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01091};
KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01091,
KW ECO:0000313|EMBL:ERJ07626.1}.
FT DOMAIN 8..291
FT /note="Luciferase-like"
FT /evidence="ECO:0000259|Pfam:PF00296"
SQ SEQUENCE 326 AA; 34822 MW; 104ED31F5154FEA0 CRC64;
MKAIELTPEQ PVETVRRLAE RAERAGFDTI FASNHYNNRD PFQTLSSIAA GTETARLGPG
VVNPYETHPV RLASRMATLD ESSDGRGVFG IGAGDASTLA NLGVERDSPL RRVLETFKVA
QRLWAGETVD HDGQFVARDA SLNYEVGEIP VYVGAQGPHM LRMSGKHADG VLVNAAHPAD
LSWSADRIAE GRAERPDDRG DFDIAAYASV SVAEDEDRAR EAARPPVAFI VGSTAPPVLD
RHDLDHDRAG RIGDAIERGD FETAFEAVTP AMIDAFAIAG SKATVADRIE AAMAYVDSFV
VASPLGPDLE TAIDLAAEAL ETATAE
//
