ID F7XN90_METZD Unreviewed; 328 AA.
AC F7XN90;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=5,10-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE EC=1.5.98.2 {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase {ECO:0000256|HAMAP-Rule:MF_01091};
DE AltName: Full=Methylene-H(4)MPT reductase {ECO:0000256|HAMAP-Rule:MF_01091};
GN Name=mer {ECO:0000256|HAMAP-Rule:MF_01091};
GN OrderedLocusNames=Mzhil_0168 {ECO:0000313|EMBL:AEH60048.1};
OS Methanosalsum zhilinae (strain DSM 4017 / NBRC 107636 / OCM 62 / WeN5)
OS (Methanohalophilus zhilinae).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosalsum.
OX NCBI_TaxID=679901 {ECO:0000313|EMBL:AEH60048.1, ECO:0000313|Proteomes:UP000006622};
RN [1] {ECO:0000313|EMBL:AEH60048.1, ECO:0000313|Proteomes:UP000006622}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4017 / NBRC 107636 / OCM 62 / WeN5
RC {ECO:0000313|Proteomes:UP000006622};
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K.,
RA Ovchinnikova G., Daligault H., Detter J.C., Han C., Tapia R., Larimer F.,
RA Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T.,
RA Wu D., Spring S., Schueler E., Brambilla E., Klenk H.-P., Eisen J.A.;
RT "The complete genome of Methanosalsum zhilinae DSM 4017.";
RL Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT to
CC methyl-H(4)MPT. {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized
CC coenzyme F420-(gamma-L-Glu)(n) = 5,10-
CC methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-
CC Glu)(n); Xref=Rhea:RHEA:21144, Rhea:RHEA-COMP:12939, Rhea:RHEA-
CC COMP:14378, ChEBI:CHEBI:15378, ChEBI:CHEBI:57818, ChEBI:CHEBI:58116,
CC ChEBI:CHEBI:133980, ChEBI:CHEBI:139511; EC=1.5.98.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01091};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 1/2. {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01091}.
CC -!- SIMILARITY: Belongs to the mer family. {ECO:0000256|HAMAP-
CC Rule:MF_01091}.
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DR EMBL; CP002101; AEH60048.1; -; Genomic_DNA.
DR RefSeq; WP_013897487.1; NC_015676.1.
DR AlphaFoldDB; F7XN90; -.
DR STRING; 679901.Mzhil_0168; -.
DR GeneID; 10821765; -.
DR KEGG; mzh:Mzhil_0168; -.
DR HOGENOM; CLU_027853_5_3_2; -.
DR OrthoDB; 213164at2157; -.
DR UniPathway; UPA00640; UER00697.
DR Proteomes; UP000006622; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01097; Tetrahydromethanopterin_reductase; 1.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR HAMAP; MF_01091; F420_mer; 1.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR019946; MeH4methanopterin_reductase.
DR NCBIfam; TIGR03555; F420_mer; 1.
DR PANTHER; PTHR43244; -; 1.
DR PANTHER; PTHR43244:SF1; 5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01091};
KW Methanogenesis {ECO:0000256|HAMAP-Rule:MF_01091};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01091};
KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01091,
KW ECO:0000313|EMBL:AEH60048.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000006622}.
FT DOMAIN 12..302
FT /note="Luciferase-like"
FT /evidence="ECO:0000259|Pfam:PF00296"
SQ SEQUENCE 328 AA; 34567 MW; 37CB7262610BFD3D CRC64;
MTFGIEFVPS DPVHKIAYYA KLAEDQGFSN VWITDHFNNR DVYTTLATLA MNTNTIKLGT
GVTNPYTRNV AITASSIGSI NDISGGRAIL GIGPGDKATF DSMGISWDKP LTMTKETIAA
LREYFAGNKV SMDGEMVKVG GAKMAFKTGD IPIYMGAQGP KMLELAGEVA DGVLINASHP
KDFEVAMEKI SAGAKKAGRS MDEIDVGAYT CFSVDKDAGK AIGAAKVVVA FIVAGSPDMV
LERHGIDVEA KKKIGEAISK GDFGALMGGL VTDGMMDAFS ICGTPDECKQ RINDLLDIGV
TQIVVGSPIG PNKEKAIKLI GKEIIGGN
//
