ID F8F9J4_PAEMK Unreviewed; 261 AA.
AC F8F9J4;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Spectinomycin 9-adenylyltransferase {ECO:0000256|PIRNR:PIRNR000819};
GN OrderedLocusNames=KNP414_05159 {ECO:0000313|EMBL:AEI43683.1};
OS Paenibacillus mucilaginosus (strain KNP414).
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1036673 {ECO:0000313|EMBL:AEI43683.1, ECO:0000313|Proteomes:UP000006620};
RN [1] {ECO:0000313|Proteomes:UP000006620}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KNP414 {ECO:0000313|Proteomes:UP000006620};
RA Wang J., Hu S., Hu X., Zhang B., Dong D., Zhang S., Zhao K., Wu D.;
RT "Complete genome sequence of Paenibacillus mucilaginosus KNP414.";
RL Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AEI43683.1, ECO:0000313|Proteomes:UP000006620}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KNP414 {ECO:0000313|EMBL:AEI43683.1,
RC ECO:0000313|Proteomes:UP000006620};
RX PubMed=24158556;
RA Lu J.J., Wang J.F., Hu X.F.;
RT "Genome Sequence of Growth-Improving Paenibacillus mucilaginosus Strain
RT KNP414.";
RL Genome Announc. 1:e00881-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC Evidence={ECO:0000256|ARBA:ARBA00001672,
CC ECO:0000256|PIRNR:PIRNR000819};
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DR EMBL; CP002869; AEI43683.1; -; Genomic_DNA.
DR RefSeq; WP_013918836.1; NC_015690.1.
DR AlphaFoldDB; F8F9J4; -.
DR KEGG; pms:KNP414_05159; -.
DR PATRIC; fig|1036673.3.peg.4772; -.
DR HOGENOM; CLU_071584_1_0_9; -.
DR Proteomes; UP000006620; Chromosome.
DR GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05403; NT_KNTase_like; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR024172; AadA/Aad9.
DR InterPro; IPR025184; AadA_C.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR Pfam; PF13427; AadA_C; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
PE 4: Predicted;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|PIRNR:PIRNR000819};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotidyltransferase {ECO:0000256|PIRNR:PIRNR000819};
KW Transferase {ECO:0000256|PIRNR:PIRNR000819}.
FT DOMAIN 16..66
FT /note="Polymerase nucleotidyl transferase"
FT /evidence="ECO:0000259|Pfam:PF01909"
FT DOMAIN 147..247
FT /note="Adenylyltransferase AadA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13427"
SQ SEQUENCE 261 AA; 28945 MW; 0A2CDB991A641F2F CRC64;
MNIHAEVDTL AAFLENELKE QLTGIYLHGS LAMGGFHPEH SDIDVLVVVK RKLTQEEKKR
IAGGLLAFEE RLPGGGGVEL SVITASSLEH WKHPAPFEFH YSAAHRDRYR ADENYMCGGG
TDPDLAAHLT VTYHRGFALH GPAVRSVVPE IPREHYMDSI FSDIENAPEQ IAAKPVYYTL
NLCRVLYYLR EGVVSSKVEG GAWGLSVLPP EYRPLIRQCL DEYSGLKQQP SVHNSRLAEE
FAGYMCTAIH REAGASGSSK I
//
