ID F8MYW4_NEUT8 Unreviewed; 1566 AA.
AC F8MYW4;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=NEUTE1DRAFT_149613 {ECO:0000313|EMBL:EGO51962.1};
OS Neurospora tetrasperma (strain FGSC 2508 / ATCC MYA-4615 / P0657).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=510951 {ECO:0000313|EMBL:EGO51962.1};
RN [1] {ECO:0000313|EMBL:EGO51962.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FGSC 2508 {ECO:0000313|EMBL:EGO51962.1};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Ellison C.E., Stajich J.E., Jacobson D.J., Lindquist E., Lapidus A.,
RA Foster B., Aerts A., Riley R., Grigoriev I.V., Taylor J.W.;
RT "Massive changes in genome architecture accompany the transition to self-
RT fertility in the filamentous fungus Neurospora tetrasperma.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR EMBL; GL891382; EGO51962.1; -; Genomic_DNA.
DR RefSeq; XP_009855606.1; XM_009857304.1.
DR GeneID; 20827134; -.
DR KEGG; nte:NEUTE1DRAFT149613; -.
DR VEuPathDB; FungiDB:NEUTE1DRAFT_149613; -.
DR HOGENOM; CLU_001517_2_1_1; -.
DR OrthoDB; 1427975at2759; -.
DR Proteomes; UP000008065; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 41..142
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 146..588
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 755..999
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1089..1545
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
FT REGION 641..660
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 646..660
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1566 AA; 176309 MW; 966E43A479B820CB CRC64;
MIPRTMVSNE VYLLPLKDDG SPDVPGEYIY IAPKSKDPVT IRFAIEGTSS ICSHGSLWVN
IPEHGEQFQR NKFREYKLVP DFNRTLEISI PIYEAGAYAF YTTYAELPDL ASSLVNADGT
VTKATQKKTP LYYIDVAPRL SLDGQPLPLP ALSIFSIISK FMGKYPTDWE RHLRGISDRG
YNMIHFTPLQ VRGASNSPYS LYDQLGWDPA CFPAGEPDVQ KMVESLEKNH SLLSLTDIVL
NHTAHNSEWL LEHPEAGYNL TTAPWLESAY LLDTKLLELG TRLEELGLPT ELKDVDDLVK
IMDAIKKEVI AEIRLWEYYT LDVERDADAA VKSWAANDID FPQASVGAGG IDSLHSATPK
EQADFLIQHG LQNMDYLGER FRRRANPKVA AALLSAIFGR YEGETKDTAD QAAARTKIVD
ILEIVNVPFY KEYDDEVAEI LQQLFNRIKY VRLDDHGPKL GPINAENPLI ETYFTRLPVN
EKTKKHKKED LVLANNGWVW GGNALVDNAG PDSRVYLRRE VIVWGDCTKL RYGSGPEDSP
WLWEHMTKYA RMLAKYFAGF RIDNCHSTPL HVAEHILDEA RRVRPDLYVV AELFTGSEEM
DYVFVKRLGI SALIREAMQA WSTGELSRLV HRHGGRPIGS FEVDEVSSNE GRSSSISGTN
GDGVYTREVI RRIRPVPVQA LFMDCTHDNE VPAQKRDARD TLPNAALVAM CASATGSVMG
YDEIYPKLVD LVGETRLYTF EASKAPVKTG EGKDGIAGVK KLLNQIHTLM GMDGYDETHI
HHEDEYVTVH RVHQESRKGY FLIAHTAFPG YGNGNGAFNP VHLTGTKARH LGSWMLEVDA
SKEAVEEVLG DKKHLRGLPS RLVGLPGVRM EVKGQDTIIT VREKFPPGSI ALFETWIPAA
EHSSGLDNFV TSGAKAAMDE LDLVDLNFLL YKCEPEERDA SEGQDGTYDI PGHGKIVYAG
LQGWWSILKD VIKDNNLAHP LCQHLRDGQW ALDYIVGRLE RASKKEDFRR LAKPAQWLKE
RFDAIRPIPS FLLPRYFGLV LRTAYNAAFE RGISLMNNNV IKGQWFLQSL AMVSVQMTGL
VKSASLYPNR LVPSLAAGLP HFAVEWARCW GRDVFISLRG LYLGTGRYAE AREHIHAFAS
VLKHGMIPNL LGSGNNPRYN SRDSVWFFLQ CIQDYTRLVP DGLSILDDKV KRRFLPYDDT
YFDVDDERAY SKESTIAEII QEVFQRHAEG MKYREANAGP NLDMQMSDAG FNQEIKVDWS
NGFIFGGNQA NCGTWMDKMG ESERAGSKGV PGTPRDGAAV EITGMLYSNL KWAASLNEAG
KFKHSSVRKS DGTEITLKDW AALIKDNFER CYFVPISPDE DSKYDVNPAI INRRGIYKDL
YRSGKEYEDY QLRSNFPIAM TCAADLFVPE HAMHALWVAD AALRGPTGMA TLDPSDMNYR
PYYNNSEDSD DFATSKGRNY HQGPEWLWPT GFFLRALLKF DLMRRGRDDA EGRTEAFQQV
TRRLIGCKEM IQRSPWAGLT ELTNKNGEEC HDSSPTQAWS AGCLIDLYMD AAEEQAKLEK
HSLPLR
//
