ID   F9P7R6_STRCV            Unreviewed;       834 AA.
AC   F9P7R6;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   27-MAR-2024, entry version 43.
DE   RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE            EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE   AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE   AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN   ORFNames=HMPREF1042_1929 {ECO:0000313|EMBL:EGV08387.1};
OS   Streptococcus constellatus subsp. pharyngis SK1060 = CCUG 46377.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus; Streptococcus anginosus group.
OX   NCBI_TaxID=1035184 {ECO:0000313|EMBL:EGV08387.1, ECO:0000313|Proteomes:UP000003287};
RN   [1] {ECO:0000313|EMBL:EGV08387.1, ECO:0000313|Proteomes:UP000003287}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SK1060 {ECO:0000313|EMBL:EGV08387.1,
RC   ECO:0000313|Proteomes:UP000003287};
RA   Harkins D.M., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA   Nelson K.E.;
RL   Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC         amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC         of amylopectin and glycogen.; EC=3.2.1.41;
CC         Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR   EMBL; AFUP01000004; EGV08387.1; -; Genomic_DNA.
DR   AlphaFoldDB; F9P7R6; -.
DR   eggNOG; COG1523; Bacteria.
DR   Proteomes; UP000003287; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 2.60.40.1220; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR011838; Pullulan_Gpos.
DR   InterPro; IPR040806; SpuA_C.
DR   NCBIfam; TIGR02102; pullulan_Gpos; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF18033; SpuA_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
PE   4: Predicted;
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          237..622
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   834 AA;  93524 MW;  08366E36B37ABF03 CRC64;
     MTGAQHIGLT EIEATFSTLE NAKKEDILKN LKITDKNKKE VAIKDVILDV KNKKAKIVGD
     FGQAQAPYTI KYGNDQFTTS MNWQLKDSIY KYDGELGARV SQAGKQVDVT FWSPSADQVD
     LVIYDKNDQN KIVGKVVMTK GEAGTWKGTL TSQTNLGIKD YRGYFYHYEI TRGGKKVLAL
     DPYAKSLAAW NSEDAEKGES YKIAKAAFVE PSEYGPKDLT YAKIPNFKKR EDALIYETHV
     RDFTSDPAIS KDLKSQFGTF SAFIEKLDYL KDLGVTHIQL LPVLSYYFVN ELKNAERMDK
     YASSNSNYNW GYDPQNYFSL TGMYSTNPKD PAKRIEEFKN LVNEIHKRGM GVIMDVVYNH
     TAKTSIFEDL EPNYYHFMDA DGTPRTSFGG GRLGTTHYMS RRVLVDSIKY LTSEYKVDGF
     RFDMMGDHDA ESIQKAYEEA KKLNPNLVML GEGWKTYTGD ENKPVQPADQ SWMKSTDTVG
     VFSDDIRNTL KSGYPNEGAP AFITGGKRNV ESVFKNIKAQ PTNFEADSPG DAIQYIAAHD
     NLTLFDIIAQ SIKKDPAVPA NNQEIHRRLR LGNLMVLTSQ GTPFIHSGQE YGRTKQFRDP
     AYKYPVPDDK VPNKSHLLTN ADGTPFEYPY FIHDSYDSSD AVNHFDWTKA TDSGKYPENA
     KSRAYMKGLI ALRKSTDAFT RKTKAEVDQN VTLITQPGKD GVGKEDLILG YQVVASNGDI
     YAVLINADSK ERKFNFGEGY KHLAQAEIVA DANTAGVAAI SNPAGVNLGA DGVSLAPLTA
     TILRIQKRVN QIKKNQTGKI NIKADFLDQK KILGKQRLLQ HREILQLRRS SNSK
//