ID FSBP_HUMAN Reviewed; 299 AA.
AC O95073; Q8N4S5;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 24-JAN-2024, entry version 166.
DE RecName: Full=Fibrinogen silencer-binding protein;
GN Name=FSBP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Hu C.-H., Cao Z., Chung D.W., Davie E.W.;
RT "A novel transcription repressor that down-regulates the expression of the
RT gamma chain of human fibrinogen.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LYS-226.
RG SeattleSNPs variation discovery resource;
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [5]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, AND INTERACTION WITH
RP APBA1.
RX PubMed=20531236; DOI=10.1097/wnr.0b013e32833bfca0;
RA Lau K.F., Perkinton M.S., Rodriguez L., McLoughlin D.M., Miller C.C.;
RT "An X11alpha/FSBP complex represses transcription of the GSK3beta gene
RT promoter.";
RL NeuroReport 21:761-766(2010).
RN [6]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-94, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: Transcriptional repressor that down-regulates the expression
CC of the fibrinogen gamma chain. Represses transcription of GSK3B gene
CC promoter via its interaction with APBA1. {ECO:0000269|PubMed:20531236}.
CC -!- SUBUNIT: Interacts with APBA1 (via PDZ 1 and 2 domains).
CC {ECO:0000269|PubMed:20531236}.
CC -!- INTERACTION:
CC O95073; Q96IX9: ANKRD36BP1; NbExp=3; IntAct=EBI-1059030, EBI-744859;
CC O95073; Q8N5M1: ATPAF2; NbExp=3; IntAct=EBI-1059030, EBI-1166928;
CC O95073; Q9UJX2: CDC23; NbExp=3; IntAct=EBI-1059030, EBI-396137;
CC O95073; Q9H0I2: ENKD1; NbExp=3; IntAct=EBI-1059030, EBI-744099;
CC O95073; Q53SE7: FLJ13057; NbExp=3; IntAct=EBI-1059030, EBI-10172181;
CC O95073; O95073: FSBP; NbExp=3; IntAct=EBI-1059030, EBI-1059030;
CC O95073; Q13131: PRKAA1; NbExp=3; IntAct=EBI-1059030, EBI-1181405;
CC O95073; P63165: SUMO1; NbExp=3; IntAct=EBI-1059030, EBI-80140;
CC O95073; Q8N8B7: TCEANC; NbExp=3; IntAct=EBI-1059030, EBI-954696;
CC O95073; Q9H614; NbExp=3; IntAct=EBI-1059030, EBI-10249899;
CC O95073-2; Q8N5M1: ATPAF2; NbExp=3; IntAct=EBI-10696047, EBI-1166928;
CC O95073-2; P42858: HTT; NbExp=3; IntAct=EBI-10696047, EBI-466029;
CC O95073-2; P16885: PLCG2; NbExp=2; IntAct=EBI-10696047, EBI-617403;
CC O95073-2; Q9NP31: SH2D2A; NbExp=3; IntAct=EBI-10696047, EBI-490630;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20531236}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O95073-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95073-2; Sequence=VSP_010774;
CC -!- TISSUE SPECIFICITY: Expressed in multiple tissues including brain.
CC {ECO:0000269|PubMed:20531236}.
CC -!- MISCELLANEOUS: Intragenic, in the second intron of RAB54B gene.
CC -!- WEB RESOURCE: Name=SeattleSNPs;
CC URL="http://pga.gs.washington.edu/data/fsbp/";
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DR EMBL; AF007866; AAD09313.1; -; mRNA.
DR EMBL; AF487652; AAL96666.1; -; Genomic_DNA.
DR EMBL; AC023632; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC033710; AAH33710.2; -; mRNA.
DR CCDS; CCDS59106.1; -. [O95073-1]
DR RefSeq; NP_001192191.1; NM_001205262.2.
DR RefSeq; NP_001243070.1; NM_001256141.1. [O95073-1]
DR AlphaFoldDB; O95073; -.
DR SMR; O95073; -.
DR BioGRID; 117321; 50.
DR BioGRID; 3188904; 5.
DR IntAct; O95073; 21.
DR MINT; O95073; -.
DR iPTMnet; O95073; -.
DR PhosphoSitePlus; O95073; -.
DR BioMuta; FSBP; -.
DR EPD; O95073; -.
DR jPOST; O95073; -.
DR MassIVE; O95073; -.
DR PaxDb; 9606-ENSP00000420405; -.
DR PeptideAtlas; O95073; -.
DR ProteomicsDB; 50644; -. [O95073-1]
DR ProteomicsDB; 50645; -. [O95073-2]
DR Antibodypedia; 65416; 61 antibodies from 16 providers.
DR DNASU; 25788; -.
DR Ensembl; ENST00000481490.3; ENSP00000420405.2; ENSG00000265817.4. [O95073-1]
DR GeneID; 100861412; -.
DR GeneID; 25788; -.
DR KEGG; hsa:100861412; -.
DR MANE-Select; ENST00000481490.3; ENSP00000420405.2; NM_001256141.2; NP_001243070.1.
DR UCSC; uc003ygm.4; human. [O95073-1]
DR AGR; HGNC:17228; -.
DR AGR; HGNC:43653; -.
DR CTD; 100861412; -.
DR CTD; 25788; -.
DR DisGeNET; 100861412; -.
DR DisGeNET; 25788; -.
DR GeneCards; FSBP; -.
DR HGNC; HGNC:43653; FSBP.
DR HPA; ENSG00000265817; Tissue enriched (retina).
DR MIM; 616306; gene.
DR neXtProt; NX_O95073; -.
DR OpenTargets; ENSG00000265817; -.
DR VEuPathDB; HostDB:ENSG00000265817; -.
DR eggNOG; ENOG502QUKT; Eukaryota.
DR GeneTree; ENSGT00390000008778; -.
DR HOGENOM; CLU_080744_0_0_1; -.
DR InParanoid; O95073; -.
DR OMA; IPRRDEF; -.
DR OrthoDB; 4072166at2759; -.
DR PhylomeDB; O95073; -.
DR TreeFam; TF331896; -.
DR PathwayCommons; O95073; -.
DR SignaLink; O95073; -.
DR BioGRID-ORCS; 100861412; 12 hits in 1133 CRISPR screens.
DR BioGRID-ORCS; 25788; 15 hits in 1156 CRISPR screens.
DR GeneWiki; RAD54B; -.
DR Pharos; O95073; Tdark.
DR PRO; PR:O95073; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; O95073; Protein.
DR Bgee; ENSG00000265817; Expressed in pigmented layer of retina and 113 other cell types or tissues.
DR ExpressionAtlas; O95073; baseline and differential.
DR Genevisible; O95073; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR InterPro; IPR042383; FSBP.
DR InterPro; IPR028002; Myb_DNA-bind_5.
DR PANTHER; PTHR15386; FIBRINOGEN SILENCER-BINDING PROTEIN; 1.
DR PANTHER; PTHR15386:SF0; FIBRINOGEN SILENCER-BINDING PROTEIN; 1.
DR Pfam; PF13873; Myb_DNA-bind_5; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isopeptide bond; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..299
FT /note="Fibrinogen silencer-binding protein"
FT /id="PRO_0000087352"
FT CROSSLNK 94
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..141
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010774"
FT VARIANT 226
FT /note="R -> K (in dbSNP:rs3136422)"
FT /evidence="ECO:0000269|Ref.2"
FT /id="VAR_019301"
SQ SEQUENCE 299 AA; 34768 MW; 3CC89EC32A377B2F CRC64;
MVGKARSSNF TLSEKLDLLK LVKPYVKILE EHTNKHSVIV EKNRCWDIIA VNYNAIGVDR
PPRTAQGLRT LYKRLKEYAK QELLQQKETQ SDFKSNISEP TKKVMEMIPQ ISSFCLVRDR
NHIQSANLDE EAQAGTSSLQ VMLDHHPVAI TVEVKQEEDI KPPPPLVLNS QQSDTLEQRE
EHELVHVMER SLSPSLSSVD MRMTSSPSSI PRRDDFFRHE SGEHFRSLLG YDPQILQMLK
EEHQIILENQ KNFGLYVQEK RDGLKRRQQL EEELLRAKIE VEKLKAIRLR HDLPEYNSL
//
