ID FTR_METTH Reviewed; 297 AA.
AC P21348; O27327;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 27-MAR-2024, entry version 140.
DE RecName: Full=Formylmethanofuran--tetrahydromethanopterin formyltransferase {ECO:0000255|HAMAP-Rule:MF_00579};
DE Short=Ftr {ECO:0000255|HAMAP-Rule:MF_00579};
DE EC=2.3.1.101 {ECO:0000255|HAMAP-Rule:MF_00579};
DE AltName: Full=H4MPT formyltransferase {ECO:0000255|HAMAP-Rule:MF_00579};
GN Name=ftr {ECO:0000255|HAMAP-Rule:MF_00579}; OrderedLocusNames=MTH_1259;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-61.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=2403564; DOI=10.1016/s0021-9258(19)40254-8;
RA Dimarco A.A., Sment K.A., Konisky J., Wolfe R.S.;
RT "The formylmethanofuran:tetrahydromethanopterin formyltransferase from
RT Methanobacterium thermoautotrophicum delta H. Nucleotide sequence and
RT functional expression of the cloned gene.";
RL J. Biol. Chem. 265:472-476(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Catalyzes the reversible transfer of a formyl group from
CC formylmethanofuran (formyl-MFR) to tetrahydromethanopterin (H(4)MPT) to
CC produce 5-formyl tetrahydromethanopterin (5-formyl-H(4)MPT) and
CC methanofuran (MFR). {ECO:0000255|HAMAP-Rule:MF_00579}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran
CC = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin;
CC Xref=Rhea:RHEA:18061, ChEBI:CHEBI:15378, ChEBI:CHEBI:57727,
CC ChEBI:CHEBI:58018, ChEBI:CHEBI:58103, ChEBI:CHEBI:58151;
CC EC=2.3.1.101; Evidence={ECO:0000255|HAMAP-Rule:MF_00579};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 2/3.
CC {ECO:0000255|HAMAP-Rule:MF_00579}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00579}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00579}.
CC -!- SIMILARITY: Belongs to the FTR family. {ECO:0000255|HAMAP-
CC Rule:MF_00579}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB85748.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; J05173; AAA88222.1; -; Genomic_DNA.
DR EMBL; AE000666; AAB85748.1; ALT_INIT; Genomic_DNA.
DR PIR; C69035; C69035.
DR RefSeq; WP_048061010.1; NC_000916.1.
DR AlphaFoldDB; P21348; -.
DR SMR; P21348; -.
DR STRING; 187420.MTH_1259; -.
DR PaxDb; 187420-MTH_1259; -.
DR EnsemblBacteria; AAB85748; AAB85748; MTH_1259.
DR GeneID; 77401786; -.
DR KEGG; mth:MTH_1259; -.
DR PATRIC; fig|187420.15.peg.1239; -.
DR HOGENOM; CLU_081314_0_0_2; -.
DR InParanoid; P21348; -.
DR BRENDA; 2.3.1.101; 3256.
DR UniPathway; UPA00640; UER00693.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030270; F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.520; -; 2.
DR HAMAP; MF_00579; FTR; 1.
DR InterPro; IPR014053; ForMFR_H4MPT_ForTrfase.
DR InterPro; IPR002770; ForMFR_H4MPT_ForTrfase_C.
DR InterPro; IPR023447; ForMFR_H4MPT_ForTrfase_fd-like.
DR InterPro; IPR022667; ForMFR_H4MPT_ForTrfase_N.
DR NCBIfam; TIGR03119; one_C_fhcD; 1.
DR Pfam; PF01913; FTR; 1.
DR Pfam; PF02741; FTR_C; 1.
DR PIRSF; PIRSF006414; Ftr_formyl_trnsf; 1.
DR SUPFAM; SSF55112; Formylmethanofuran:tetrahydromethanopterin formyltransferase; 2.
PE 1: Evidence at protein level;
KW Acyltransferase; Cytoplasm; Direct protein sequencing; Methanogenesis;
KW One-carbon metabolism; Reference proteome; Transferase.
FT CHAIN 1..297
FT /note="Formylmethanofuran--tetrahydromethanopterin
FT formyltransferase"
FT /id="PRO_0000138122"
FT CONFLICT 28
FT /note="Missing (in Ref. 1; AAA88222)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="E -> Q (in Ref. 1; AAA88222)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 31470 MW; AB9DBDA9809552D6 CRC64;
MEINGVEIED TFAEAFGIKV SRVLVTAATK KLAKIAATEA TGYGTSVIGC PAEAGIDCYV
PPEETPDGRP GYIIMICNPS KKSLDHELLE RIGMGILTAP TTAVFDALDD EDEKLNIGFK
LKFFGDGYEK ELEIDGRKIH SIPIMSGDFL IESEFGIKDG VAGGNFFIMG DSQASALLAA
QAAVDAIAAV EGTVTPFPGG VVASGSKVGS NKYKFLNAST NEKMCVTLKD EVEDTQIPEN
VNGVYEIVID GVDEEAVREA MKEGIKAACT VPGIIKISAG NYGGNLGAYK IKLHDLF
//
