ID   G0NEI3_CAEBE            Unreviewed;       971 AA.
AC   G0NEI3;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   24-JAN-2024, entry version 55.
DE   SubName: Full=CBN-ICL-1 protein {ECO:0000313|EMBL:EGT58826.1};
GN   Name=Cbn-icl-1 {ECO:0000313|EMBL:EGT58826.1};
GN   ORFNames=CAEBREN_24884 {ECO:0000313|EMBL:EGT58826.1};
OS   Caenorhabditis brenneri (Nematode worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=135651 {ECO:0000313|Proteomes:UP000008068};
RN   [1] {ECO:0000313|Proteomes:UP000008068}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PB2801 {ECO:0000313|Proteomes:UP000008068};
RG   Caenorhabditis brenneri Sequencing and Analysis Consortium;
RA   Wilson R.K.;
RL   Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC         Evidence={ECO:0000256|ARBA:ARBA00001699};
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 1/2. {ECO:0000256|ARBA:ARBA00004793}.
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DR   EMBL; GL379873; EGT58826.1; -; Genomic_DNA.
DR   AlphaFoldDB; G0NEI3; -.
DR   STRING; 135651.G0NEI3; -.
DR   EnsemblMetazoa; CBN24884.1; CBN24884.1; WBGene00163609.
DR   eggNOG; KOG1260; Eukaryota.
DR   eggNOG; KOG1261; Eukaryota.
DR   HOGENOM; CLU_012853_0_0_1; -.
DR   InParanoid; G0NEI3; -.
DR   OMA; IWMETSH; -.
DR   Proteomes; UP000008068; Unassembled WGS sequence.
DR   GO; GO:0004451; F:isocitrate lyase activity; IEA:InterPro.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00377; ICL_PEPM; 1.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR   Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR   Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR   InterPro; IPR039556; ICL/PEPM.
DR   InterPro; IPR006254; Isocitrate_lyase.
DR   InterPro; IPR018523; Isocitrate_lyase_ph_CS.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR001465; Malate_synthase_TIM.
DR   InterPro; IPR048355; MS_C.
DR   InterPro; IPR048356; MS_N.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   NCBIfam; TIGR01346; isocit_lyase; 1.
DR   NCBIfam; TIGR01344; malate_syn_A; 1.
DR   PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR   PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR   Pfam; PF00463; ICL; 1.
DR   Pfam; PF20659; MS_C; 1.
DR   Pfam; PF20656; MS_N; 1.
DR   Pfam; PF01274; MS_TIM-barrel; 1.
DR   Pfam; PF13714; PEP_mutase; 1.
DR   SUPFAM; SSF51645; Malate synthase G; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00161; ISOCITRATE_LYASE; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   4: Predicted;
KW   Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008068};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT   DOMAIN          454..507
FT                   /note="Malate synthase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF20656"
FT   DOMAIN          601..842
FT                   /note="Malate synthase TIM barrel"
FT                   /evidence="ECO:0000259|Pfam:PF01274"
FT   DOMAIN          850..966
FT                   /note="Malate synthase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF20659"
FT   ACT_SITE        604
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601465-50"
FT   ACT_SITE        884
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601465-50"
SQ   SEQUENCE   971 AA;  108848 MW;  768FB935BBB14EB1 CRC64;
     MSTGGAAAKN FYQVVKSAPK GRFKGIKRDY TVEDVLKLRG SIDIDYTLAT RGANKLWQLL
     HTEPFVPALG AQTGNQAVQM VRAGLKAIYL SGWQVAADAN SAGDMYPDQS LYPANSGPDL
     ARRINRSLRR ADQIEACEAE DYLAQRDWYA PIVADAEAGF GGALNCFELM KAYIEAGASG
     VHYEDQLGSE KKCGHMGGKV LIPTAQHIRH LNASRLAADV CGVPTIIVAR TDAESSRLLT
     SDIDPRDHPY IDYEAGRTIE GFYRLKDSTA IQYCVDRAIQ YAPYCDLIWM ETSHPTIADA
     REFAEGVRKV YPDKMFAYNC SPSFNWKKHL SPQQMEKFQK ELGAMGFKYQ FITLAGFHAN
     SYSMFDLARN YKETGMLAYS ALQEGEFAAE KHGYTAVKHQ REVGTGYFDA VSRAVTGGLS
     STTALSGSTE EAQFQTAVAS EDEEILSLTA QNVAGDEKIL TPDALRFLHD LNTEFNPRRL
     RLLSKRRTVQ DDINNSIWFP DFNKETEVLR SDQAWKGAEI PRDLQDRRVE ITGPTDRKMV
     INAMNSGANV FMADFEDSNS PTWRNQLEGQ INLYDAVRKN ISYTHPTTKK EYTLNEKHAV
     LKVRPRGWHL PEKHVLIHNQ PTSGSLFDFG LFVFHNAKAL IAQGSGPYFY LPKLQSAEEA
     QLWADVFKYT EEKLGLARGT IKCTVLIEHL LASFQLHEII HALKDNIVGL NCGRWDYIFS
     YIKTFQAHRK FLLPDRFQIG MTAPFMRNYS LEVIKACHQR GIHAMGGMAA QIPIKHDQVA
     NDKAFALVRA DKEREVTDGH DGTWVAHPGL VPLAKGVFDQ MMPKPNQINK ILSRSPCTKE
     DLTVIPEGSR TEAGFRHNIS VTLGYLDSWL RGTGCVPLYN LMEDAATAEI SRAQLWQWLH
     HDAKLEDGRT IDAGLVKQTI AAESERRLIR AGSVVNRIPE AAELLEKFVT EEKMSDFLTT
     DAYDRLVSEG Y
//