ID G0S0I6_CHATD Unreviewed; 697 AA.
AC G0S0I6;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=PUM-HD domain-containing protein {ECO:0000259|PROSITE:PS50303};
GN ORFNames=CTHT_0010150 {ECO:0000313|EMBL:EGS23347.1};
OS Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
OS (Thermochaetoides thermophila).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Chaetomiaceae; Thermochaetoides.
OX NCBI_TaxID=759272 {ECO:0000313|Proteomes:UP000008066};
RN [1] {ECO:0000313|EMBL:EGS23347.1, ECO:0000313|Proteomes:UP000008066}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1495 / CBS 144.50 / IMI 039719
RC {ECO:0000313|Proteomes:UP000008066};
RX PubMed=21784248; DOI=10.1016/j.cell.2011.06.039;
RA Amlacher S., Sarges P., Flemming D., van Noort V., Kunze R., Devos D.P.,
RA Arumugam M., Bork P., Hurt E.;
RT "Insight into structure and assembly of the nuclear pore complex by
RT utilizing the genome of a eukaryotic thermophile.";
RL Cell 146:277-289(2011).
RN [2] {ECO:0007829|PDB:8IA0}
RP STRUCTURE BY ELECTRON MICROSCOPY (2.70 ANGSTROMS).
RX PubMed=37129998; DOI=10.15252/embr.202356910;
RA Lau B., Huang Z., Kellner N., Niu S., Berninghausen O., Beckmann R.,
RA Hurt E., Cheng J.;
RT "Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation
RT during pre-60S biogenesis.";
RL EMBO Rep. 24:e56910-e56910(2023).
CC -!- FUNCTION: RNA-binding nucleolar protein required for pre-rRNA
CC processing. Involved in production of 18S rRNA and assembly of small
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00024893}.
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DR EMBL; GL988037; EGS23347.1; -; Genomic_DNA.
DR RefSeq; XP_006691538.1; XM_006691475.1.
DR PDB; 8IA0; EM; 2.70 A; CZ=1-697.
DR AlphaFoldDB; G0S0I6; -.
DR EMDB; EMD-35290; -.
DR STRING; 759272.G0S0I6; -.
DR GeneID; 18255053; -.
DR KEGG; cthr:CTHT_0010150; -.
DR eggNOG; KOG2050; Eukaryota.
DR HOGENOM; CLU_013994_1_0_1; -.
DR OMA; YGPEFSI; -.
DR OrthoDB; 5479527at2759; -.
DR Proteomes; UP000008066; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012959; CPL_dom.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR040059; PUM3.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13389:SF0; PUMILIO HOMOLOG 3; 1.
DR PANTHER; PTHR13389; UNCHARACTERIZED; 1.
DR Pfam; PF08144; CPL; 1.
DR SMART; SM00025; Pumilio; 5.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR PROSITE; PS50302; PUM; 1.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:8IA0};
KW Reference proteome {ECO:0000313|Proteomes:UP000008066};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 119..496
FT /note="PUM-HD"
FT /evidence="ECO:0000259|PROSITE:PS50303"
FT REPEAT 184..219
FT /note="Pumilio"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00317"
FT REGION 1..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 651..689
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..46
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..114
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 657..676
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 697 AA; 77342 MW; 83982BA6822F99EA CRC64;
MAQKSANSAA PAKRKSISSD KHADSNRVKK AKTENGPKHK KFDDTEIAPN SGAGDFSDVE
DGRNQQKPMK NIKTGGTSKE NDKTTGKPAG VSSREAHARQ KQLEKERKAA KPLAEELART
KQLWETLRRK SHVPKEQRQK LIEELYSIIT GRIKDFVLKH DAVRAVQTAI KYATPTQRRQ
IAKELQGSYA QLAESRYAKF LIAKLLVQND EEIRDMIIPE FFGKVRKLIN HPEASWILDD
IYRTVATKQQ KAQMLREWYG PEFALFKPEK GAEATADLSK ILAEEPSKRA PVLKYLFDMT
NTLIQKKMTG FTMLHDAMLQ YFLNLKPESE ELKEFVEVIK GDEGGDLLKN LAFTKSGARL
VCLLLAHGNA KDRKQILKTY KDTFQLMCGD QYAHMVILTA YDVIDDTVMT AKTIFPELLG
RNEEKNLENI IFLANDLTAR ITVMYLFEGQ AKSLFPASHA YDLELLAEIH EIRKKTSKKD
PEVRRKELVA AVSPPLLAAV AASPKDLVST GFGCQFVADV LLAATGDKKA AMEAVASTAA
GDPNAPPPED ADPVNLPLPH LSLTTHGGKM LKTLIAGGRF DKEAGCVKRV DPPLNFADIL
YPVIKEHIVK WAIGPSSFTV LGMLEAPDFS LKKELIKTLK AERKTLEAAA NGELSSHEIK
CENKPDGKKK NKAKANGSEG KPIGNRGSKL ILEKLGD
//
