ID G1EEQ5_9INFA Unreviewed; 756 AA.
AC G1EEQ5;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:AEI29979.1};
OS Influenza A virus (A/duck/Korea/U8-1/2007(H3N8)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=1041911 {ECO:0000313|EMBL:AEI29979.1};
RN [1] {ECO:0000313|EMBL:AEI29979.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/duck/Korea/U8-1/2007 {ECO:0000313|EMBL:AEI29979.1};
RX PubMed=21955449; DOI=10.1016/j.vetmic.2011.08.028;
RA Choi J.G., Kang H.M., Kim M.C., Paek M.R., Kim H.R., Kim B.S., Kwon J.H.,
RA Kim J.H., Lee Y.J.;
RT "Genetic relationship of H3 subtype avian influenza viruses isolated from
RT domestic ducks and wild birds in Korea and their pathogenic potential in
RT chickens and ducks.";
RL Vet. Microbiol. 155:147-157(2012).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; JN087150; AEI29979.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 285..482
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AEI29979.1"
SQ SEQUENCE 756 AA; 86191 MW; 8A2BF7AB6C4387BF CRC64;
DVNPTLLFLK VPAQNAISTT FPYTGDPPYS HGTGTGYTMD TVNRTHQYSE KGKWTTNTET
GAPQLNPIDG PLPEDNEPSG YAQTDCVLEA MAFLEESHPG IFENSCLETM EVVQQTRVDK
LTQGRQTYDW TLNRNQPAAT ALANTIEVFR SNGLTANESG RLIDFLKDVM ESMNKEGMEI
TTHFQRKRRV RDNMTKKMVT QRTIGKKKQR LNKKSYLIRA LTLNTMTKDA ERGKLKRRAI
ATPGMQIRGF VYFVETLARS ICEKLEQSGL PVGGNEKKAK LANVVRKMMT NSQDTELSFT
ITGDNTKWNE NQNPRMFLAM ITYITRKQPE WFRNVLSIAP IMFSNKMARL GKGYMFESKS
MKLRTQIPAE MLANIDLKYF NESTRKKIEK IRPLLIDGTA ALSPGMMMGM FNMLSTVLGV
SILNLGQKRY TKTTYWWDGL QSSDDFALIV NAPNHEGVQA GVDRFYRTCK LVGINMSKKK
SYINRTGTFE FTSFFYRYGF VANFSMELPS FGVSGINESA DMSIGVTVIK NNMINNDLGP
ATAQMALQLF IKDYRYTYRC HRGDTQIQTR RSFELKKLWE QTRSKAGLLV SDGGPNLYNI
RNLHIPEVCL KWELMDEDYQ GRLCNPLNPF VSHKEIESVN NAVVMPAHGP AKSMEYDAVA
TTHSWIPKRN RSILNTSQRG ILEDEQMYQK CCNLFEKFFP SSSYRRPVGI SSMVEAMVSR
ARIDARIDFE SGRINKEEFA EIMKICSTIE ELRRQK
//
