ID G1N2L2_MELGA Unreviewed; 832 AA.
AC G1N2L2;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 3.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS Meleagris gallopavo (Wild turkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Meleagridinae; Meleagris.
OX NCBI_TaxID=9103 {ECO:0000313|Ensembl:ENSMGAP00000005885.3, ECO:0000313|Proteomes:UP000001645};
RN [1] {ECO:0000313|Ensembl:ENSMGAP00000005885.3, ECO:0000313|Proteomes:UP000001645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20838655; DOI=10.1371/journal.pbio.1000475;
RA Dalloul R.A., Long J.A., Zimin A.V., Aslam L., Beal K., Blomberg L.A.,
RA Bouffard P., Burt D.W., Crasta O., Crooijmans R.P., Cooper K.,
RA Coulombe R.A., De S., Delany M.E., Dodgson J.B., Dong J.J., Evans C.,
RA Frederickson K.M., Flicek P., Florea L., Folkerts O., Groenen M.A.,
RA Harkins T.T., Herrero J., Hoffmann S., Megens H.J., Jiang A., de Jong P.,
RA Kaiser P., Kim H., Kim K.W., Kim S., Langenberger D., Lee M.K., Lee T.,
RA Mane S., Marcais G., Marz M., McElroy A.P., Modise T., Nefedov M.,
RA Notredame C., Paton I.R., Payne W.S., Pertea G., Prickett D., Puiu D.,
RA Qioa D., Raineri E., Ruffier M., Salzberg S.L., Schatz M.C., Scheuring C.,
RA Schmidt C.J., Schroeder S., Searle S.M., Smith E.J., Smith J.,
RA Sonstegard T.S., Stadler P.F., Tafer H., Tu Z.J., Van Tassell C.P.,
RA Vilella A.J., Williams K.P., Yorke J.A., Zhang L., Zhang H.B., Zhang X.,
RA Zhang Y., Reed K.M.;
RT "Multi-platform next-generation sequencing of the domestic turkey
RT (Meleagris gallopavo): genome assembly and analysis.";
RL PLoS Biol. 8:E1000475-E1000475(2010).
RN [2] {ECO:0000313|Ensembl:ENSMGAP00000005885.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR AlphaFoldDB; G1N2L2; -.
DR Ensembl; ENSMGAT00000006629.3; ENSMGAP00000005885.3; ENSMGAG00000005922.3.
DR GeneTree; ENSGT00390000010998; -.
DR HOGENOM; CLU_006322_1_0_1; -.
DR TreeFam; TF313888; -.
DR Proteomes; UP000001645; Chromosome Z.
DR Bgee; ENSMGAG00000005922; Expressed in duodenum and 18 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000001645}.
FT DOMAIN 64..368
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 438..803
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 832 AA; 93412 MW; CEEAE6AE5756BA1E CRC64;
GGNCRASLGR IGLAARRSAG DVPEAVSPLT ISSYFKWWYK KTRIEKKSAF IDLLCAVPLQ
QIYGCPLGGI GGGTITRGWR GEFCRWQLNP GKYHYETVIT DQFTVCLRRK GQTVYQQVLS
VEKPSALQGW NWGYCGRYAF YHALYPRAWM VYELPGQNVV LTCRQISPVI PHDYKDSSLP
VGVFIWEVEN NSEEPVDVSI MFTLQNGTGT KGDGSGGHWN EPFALQDGGE RVTGVLLHHC
TPINPFTFAV AAREKAGTVI THLTAFDPAG SGRDVWQDLL QDGKLESPIG KSKRTQKGEV
TAAAVCASCM VPARGHGTLE LALVWDMPRV HFGSKERLHL RRYTRFFGSN GDAAPALSHY
ALTHYKEWEK KIEAWQKPIL EDSQLPSWYK SALFNELYFM TDGGTIWLDL PPDCLPQDLQ
GPGAAKLSHL LPVLREYGRF AYLEGQEYRM YNTYDVHFYA SFALVMLWPK LQISLQYDIA
VTVLNEDMQP RQYLVCGKTA QVKLKNVVPH DIGDPDDEPW QRVNAYLMHD TANWKDLNLK
FVLQVYRDYY LTHDALYLQD MWPVCQAVME SELKFDTDND GLIENGGTAD QTYDAWVVNG
ASAYCGGLWL AAVRMMCEMA EVLGDAETQQ KYGAILQKGK ESFERLLWNG KYYNYDSSGS
STSSSIMSDQ CAGQWFLGAC GLDQKELEVF PKSHIVSALK TIFEKNVMSF AGGTMGAVNG
MRPDGVPDTS SVQSSEVWVG VVYALAATMI QEGLVQEGFH TAEGCYRTVW ENLGMAFQTP
EAYCEKKVYR SLAYMRPLSI WSMQLALEMA QEKQRTWFPV RAAQHCGRAP SS
//
