ID G1Q205_MYOLU Unreviewed; 388 AA.
AC G1Q205;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=Splicing factor 45 {ECO:0000256|PIRNR:PIRNR031066};
DE AltName: Full=RNA-binding motif protein 17 {ECO:0000256|PIRNR:PIRNR031066};
OS Myotis lucifugus (Little brown bat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;
OC Myotis.
OX NCBI_TaxID=59463 {ECO:0000313|Ensembl:ENSMLUP00000017738.1, ECO:0000313|Proteomes:UP000001074};
RN [1] {ECO:0000313|Ensembl:ENSMLUP00000017738.1, ECO:0000313|Proteomes:UP000001074}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSMLUP00000017738.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Splice factor that binds to the single-stranded 3'AG at the
CC exon/intron border and promotes its utilization in the second catalytic
CC step. Involved in the regulation of alternative splicing and the
CC utilization of cryptic splice sites. {ECO:0000256|PIRNR:PIRNR031066}.
CC -!- SUBUNIT: Associates with the spliceosome.
CC {ECO:0000256|PIRNR:PIRNR031066}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR031066}.
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DR EMBL; AAPE02049592; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; G1Q205; -.
DR STRING; 59463.ENSMLUP00000017738; -.
DR Ensembl; ENSMLUT00000030614.1; ENSMLUP00000017738.1; ENSMLUG00000025709.1.
DR eggNOG; KOG1996; Eukaryota.
DR GeneTree; ENSGT00790000123099; -.
DR HOGENOM; CLU_044888_0_0_1; -.
DR InParanoid; G1Q205; -.
DR OMA; IMSKFGY; -.
DR TreeFam; TF313987; -.
DR Proteomes; UP000001074; Unassembled WGS sequence.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:UniProtKB-UniRule.
DR CDD; cd12647; RRM_UHM_SPF45; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR040052; RBM17.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR InterPro; IPR034653; SPF45_RRM.
DR PANTHER; PTHR13288:SF8; SPLICING FACTOR 45; 1.
DR PANTHER; PTHR13288; SPLICING FACTOR 45 SPF45; 1.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF00076; RRM_1; 1.
DR PIRSF; PIRSF031066; Splicing_factor_SPF45; 1.
DR SMART; SM00443; G_patch; 1.
DR SMART; SM00361; RRM_1; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|PIRNR:PIRNR031066};
KW mRNA splicing {ECO:0000256|PIRNR:PIRNR031066};
KW Nucleus {ECO:0000256|PIRNR:PIRNR031066};
KW Reference proteome {ECO:0000313|Proteomes:UP000001074};
KW RNA-binding {ECO:0000256|PIRNR:PIRNR031066};
KW Spliceosome {ECO:0000256|PIRNR:PIRNR031066}.
FT DOMAIN 222..262
FT /note="G-patch"
FT /evidence="ECO:0000259|PROSITE:PS50174"
FT REGION 102..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..157
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..189
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 388 AA; 43685 MW; 4922D35F46661ED5 CRC64;
MSLYDDLGVE TSDLKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG
GSSDDRQIVD TPPHVAAGLK DLPLADEYDP MFPNDYEKVV KRQREERQRQ RELERQKEIE
EREKRRKDRH EASGFSRRPD PDSDLEDCER ERRKRSMGGA AIAPPTSLVE KDKELPREFP
YEEDSRPRSQ SSKAAIPPPV YEEQDRPRSP TGPGNSFLAN MGGTVAHKIM QKYGFREGQG
LGKHEQGLST ALSVEKTSKR GGKIIVGEVT EKDATKKSGS NPLTEILKCP TKVVLLRNMV
GAGEVDEDLE VETKEECEKY GKVGKCVIFE IPGAPDDEAV RIFLEFERVE SAIKAVIDLN
GRYFGGRVVK ACFYNLDKFR VMDLAEQV
//
