ID G1R853_NOMLE Unreviewed; 668 AA.
AC G1R853;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 2.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=G protein-coupled receptor kinase {ECO:0000256|RuleBase:RU000308};
DE EC=2.7.11.- {ECO:0000256|RuleBase:RU000308};
GN Name=GRK3 {ECO:0000313|Ensembl:ENSNLEP00000009387.2};
OS Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hylobatidae;
OC Nomascus.
OX NCBI_TaxID=61853 {ECO:0000313|Ensembl:ENSNLEP00000009387.2, ECO:0000313|Proteomes:UP000001073};
RN [1] {ECO:0000313|Ensembl:ENSNLEP00000009387.2, ECO:0000313|Proteomes:UP000001073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Gibbon Genome Sequencing Consortium;
RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSNLEP00000009387.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[beta-adrenergic receptor] + ATP = [beta-adrenergic receptor]-
CC phosphate + ADP + H(+); Xref=Rhea:RHEA:19429, Rhea:RHEA-COMP:11222,
CC Rhea:RHEA-COMP:11223, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43176, ChEBI:CHEBI:68546, ChEBI:CHEBI:456216;
CC EC=2.7.11.15; Evidence={ECO:0000256|ARBA:ARBA00036224};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19430;
CC Evidence={ECO:0000256|ARBA:ARBA00036224};
CC -!- SUBCELLULAR LOCATION: Postsynapse {ECO:0000256|ARBA:ARBA00034110}.
CC Presynapse {ECO:0000256|ARBA:ARBA00034106}. Synapse
CC {ECO:0000256|ARBA:ARBA00034103}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr
CC protein kinase family. GPRK subfamily. {ECO:0000256|ARBA:ARBA00009793,
CC ECO:0000256|RuleBase:RU000308}.
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DR EMBL; ADFV01102729; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102730; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102731; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102732; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102734; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ADFV01102735; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; G1R853; -.
DR STRING; 61853.ENSNLEP00000009387; -.
DR Ensembl; ENSNLET00000009836.2; ENSNLEP00000009387.2; ENSNLEG00000007720.2.
DR eggNOG; KOG0986; Eukaryota.
DR GeneTree; ENSGT00940000157699; -.
DR HOGENOM; CLU_000288_63_41_1; -.
DR InParanoid; G1R853; -.
DR OMA; LKCFLIA; -.
DR TreeFam; TF313940; -.
DR Proteomes; UP000001073; Chromosome 7b.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0047696; F:beta-adrenergic receptor kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0004703; F:G protein-coupled receptor kinase activity; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0031623; P:receptor internalization; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR CDD; cd01240; PH_GRK2_subgroup; 1.
DR CDD; cd08747; RGS_GRK2_GRK3; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 1.10.167.10; Regulator of G-protein Signalling 4, domain 2; 2.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 2.
DR InterPro; IPR000961; AGC-kinase_C.
DR InterPro; IPR000239; GPCR_kinase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24355:SF17; BETA-ADRENERGIC RECEPTOR KINASE 2; 1.
DR PANTHER; PTHR24355; G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF00615; RGS; 1.
DR PRINTS; PR00717; GPCRKINASE.
DR SMART; SM00233; PH; 1.
DR SMART; SM00315; RGS; 1.
DR SMART; SM00133; S_TK_X; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF48097; Regulator of G-protein signaling, RGS; 1.
DR PROSITE; PS51285; AGC_KINASE_CTER; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS50132; RGS; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU000308};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000001073};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527,
KW ECO:0000256|RuleBase:RU000308};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000308}.
FT DOMAIN 55..176
FT /note="RGS"
FT /evidence="ECO:0000259|PROSITE:PS50132"
FT DOMAIN 192..433
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 434..501
FT /note="AGC-kinase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51285"
FT DOMAIN 538..632
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT REGION 649..668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 318
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600239-51"
FT BINDING 221
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 668 AA; 77251 MW; 955B686C15300354 CRC64;
LHDLELVSLS QDIRVSFILG MVTHNVGCIV KNVSLFPSSI RSVMQKYLAE RNEITFDKIF
NQKIGFLLFK DFCLNEINEA VPQVKFYEEI KEYEKLDNEE DRLCRSRQIY DAYIMKELLS
CSHPFSKQAV EHVQSHLSKK QVTSTLFQPY IEEICESLRG DIFQKFMESD KFTRFCQWKN
VELNIHLTMN EFSVHRIIGR GGFGEVYGCR KADTGKMYAM KCLDKKRIKM KQGETLALNE
RIMLSLVSTG DCPFIVCMTY AFHTPDKLCF ILDLMNGGDL HYHLSQHGVF SEKEMRFYAT
EIILGLEHMH NRFVVYRDLK PANILLDEHG HARISDLGLA CDFSKKKPHA SVGTHGYMAP
EVLQKGTAYD SSDAVSMNGF LKSYSSFPQN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH
GGGSQEVKEH SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD
CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK RAKNKQLGHE EDYALGKDCI
MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL LTMEQILSVE ETQIKDKKCI
LFRIKGGKQF VLQCESDPEF VQWKKELNET FKEAQRLLRR APKFLNKPRS GTVELPKPSL
CHRNSNGL
//
