ID G2NA53_STREK Unreviewed; 3462 AA.
AC G2NA53;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE SubName: Full=Amino acid adenylation domain protein {ECO:0000313|EMBL:AEN08131.1};
GN ORFNames=SACTE_0181 {ECO:0000313|EMBL:AEN08131.1};
OS Streptomyces sp. (strain SirexAA-E / ActE).
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=862751 {ECO:0000313|EMBL:AEN08131.1, ECO:0000313|Proteomes:UP000001397};
RN [1] {ECO:0000313|EMBL:AEN08131.1, ECO:0000313|Proteomes:UP000001397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SirexAA-E / ActE {ECO:0000313|Proteomes:UP000001397};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Adams A., Raffa K.,
RA Adams S., Book A., Currie C., Woyke T.;
RT "Complete sequence of Streptomyces sp. SirexAA-E.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AEN08131.1, ECO:0000313|Proteomes:UP000001397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SirexAA-E / ActE {ECO:0000313|Proteomes:UP000001397};
RX PubMed=24710170; DOI=10.1371/journal.pone.0094166;
RA Takasuka T.E., Acheson J.F., Bianchetti C.M., Prom B.M., Bergeman L.F.,
RA Book A.J., Currie C.R., Fox B.G.;
RT "Biochemical properties and atomic resolution structure of a
RT proteolytically processed beta-mannanase from cellulolytic Streptomyces sp.
RT SirexAA-E.";
RL PLoS ONE 9:e94166-E94166(2014).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP002993; AEN08131.1; -; Genomic_DNA.
DR RefSeq; WP_014044123.1; NC_015953.1.
DR STRING; 862751.SACTE_0181; -.
DR KEGG; ssx:SACTE_0181; -.
DR PATRIC; fig|862751.12.peg.195; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_000022_50_0_11; -.
DR OrthoDB; 2472181at2; -.
DR Proteomes; UP000001397; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 3.
DR CDD; cd19540; LCL_NRPS-like; 2.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 3.40.50.980; -; 6.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 3.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 3.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR001031; Thioesterase.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 3.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 3.
DR Pfam; PF13193; AMP-binding_C; 3.
DR Pfam; PF00668; Condensation; 3.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 6.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001397}.
FT DOMAIN 974..1049
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2043..2118
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3113..3188
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1262..1281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2334..2356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2723..2742
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3462 AA; 368843 MW; 0F47EA7C268B821A CRC64;
MTDAPHNGTP PDTLRLTSAQ RGLWFAQRLD PADPSYNVAE YADIRGHLRP GALRRAVGHT
VAETEALRST FGERDGVPFQ RVHERAHVDV PLVDLSEHED PHTEAVRRME RFLSEPVDLG
TGPLVRMTLY RLSETHHLFH QQVHHLALDG YGAVLTLARI AEVYTALCDS PSAAPPSGPV
APLAALVAEE DAYRASHQHD ADRAFWTDHL AGAPAPTAPP VRTGPATYGA RSVRREFTAE
ETARLRAAAK RAGTGWPTFF MAALAVCQHR DRGLAEVVLG LPVTGRRTAL ARATPGMLSN
VLPMRLAVSP ADRVADLAKR ASAVARKVLR HQRHPSESLR HQLGLTGSRT PLTGPSVNIL
AFDDTLRFGP CPATLHNLSI GPVEDLAVAV HASYTGGGMR LDLLGNPGLH TPQELTAQHA
RLCRVIDAFA DDPYRTIGSL RLVGDDEHHR VLGLGATRPP AERDGDSLLP LPEQLALQAR
ATPDRTAVVC ADARLTFAQL DRQVDVLAGV LAARGARPGS RVAVGLPRST DLVVAMLAVM
RTGAAYLPLD PSYPADRLAF MIEDSRPVAL VATARSARTM DPGATVRLVD PAGAAAETER
REPVRPGPGD AAYVIYTSGS TGRPKGVVVE HRSLSNLLEH HRHEAHAPAE AALGRRLRVA
LSAATSFDAS WDPVLWMVAG HELHIVDDDV RRDPQALVDF LVEQRIDAVE TTPTYLRQML
TAGLLTDPRH RPRVIALGGE AVDDALWDEL ADDPGLLVFN YYGPTETTVD AVTTRVTGGS
PVIGRPVAGT GAYVLDPSLH QLPQGAVGEL YLTGEGLARG YAGRPGLTAE RFMPDPFGRP
GGRMYRTGDL ARWSEDDTLE FLGRNDRQIK VRGFRVETGE IEASLRSLPG VADAAVVLAS
PQDGPERLAA YLVAATKEAA PGHAEVRDAL AKVLPGHMVP AAWATVPRLP VTPNGKLDTA
GLPEAVPLAS GGAEPRTPEE ARMCAVFAEC LSLPRAGRDD DFFLLGGHSL LAMRLIGRIR
GEFGAELPIR ALFASPTPAG LVAALAEASP ARPAVAEVRP RPDPLPLSFG QRRLWLLQTL
GDTGTSYHLP VALRLRGALD RDALAHALAD LVARHESLRT VVGEGPDGPV QRILPAATRP
PLRVMGAADP VRPDPAPFDL GRNLPLRIQL FPAGPEDHLL VVTLHHIAAD GWSMGPLMRD
LSTAYAARRA GEAPEWTALP VQYADYALWQ RRLLGDVEDP GSLAGRQLAH WAGSLAGLPE
ESVFPADRPR PARPSGRGGS VPVRIGAADH RALVRLAGDC GASGFMVLHA ALAALMSRLG
AGPDMAIGTP VAGRTDEALT DLVGFFVNTL VLRTDTSGAP DFRELVRRVA RDDLAAYAHQ
DLPFERIVEE LAPARSLSRH PLFQVMLSLN NTPPPEAAFD GLSVRHEPSV GRSGAKFDLT
WDLVEHHDDR GAPAGIRGEL EFSEDLFDRG TAERFCEHFC RLLSALLADP DRPVADAGFL
TPEQRAAALD ELPRATARPL TGASSARPGG PDGEWTVVDV LETRAAVQPG RTAVVAPDGR
LPFGELLALG HRLAAALAEA GIGRGDRVAV ALPRTSLHIV ALLGVLRAGA VYVPLDPSHP
RARNQLILDT ASPRLVLATA GTRAALPGTA DVLLLDGDAP WRDARGPLPA PPRGRDAAYV
LYTSGSTGVP KGVVVEHRSL ANLLSGHRER LMEPAETGNN GLPLRVALTA AMTFDASWDP
LLWMVAGHEL HLVDDATRRD PEALTALLHE RAIDVVETTP SFVEQLRSCG LFAPGRPRPR
VLALGGEAVN DALWRELSAL NDVTVWNLYG PTETTVDSVM GRIVPGSRPH LGHPVNGTRA
RVLDARLQPV APGAVGELHL AGPGLARGYE NRPAATAARF LPDPYGAPGA RMYRTGDLVR
RKEGRLEYVG RADGQIKVRG FRVETGEIET VLADHPDVAQ SAVAVRHGAT GEGRLVGWVV
PADGREPAVR ALRAFVADRL PAHMVPGTIV PVAALPLTSH GKVDLAALPL PPKDASGAAL
DDPPRSAPEE ILCALFAESL GHEQVSRADD FFELGGHSLL ATRLVGRIRS AFGVELPVRA
LFESTTPAAL AERLGEAAAA RPALRRAVRP DRPPLSPAQR RLWFLNELDP GTAAYHISFA
VRLRGALDHR ALRLALGDVL ARHESLRTLI KESDGEPYQA VPASGDARVT LPLTPVDDAS
LDAALRDSAA LPFDLAREVP LRAALFRTAD DRHTLLVTVH HIAADGWSMG PLAQDLAGAY
AARTAGRTPS WTPLPVQYAD YTLWQKDLLG STEDPASLAT GQLAYWQAAL AGAPEETPLP
RDRPRPTASS GRGGIVALPL TEETTADLAR LARSSATSTF MVLHSALAAL LHRIGGGADV
VVGTPVAGRT DEALDPLVGF FVNTLALRTE VRAEEPFDGL LARVRETDLA AYAHQELPFE
RLVEAVRPTR SPARHPLFQV MLGLNNHRPP QLDLPGLHAR VEGVDTGSAK FDLSFSFTER
PGPRGGEAAL EGTLEFSRDL FDTGTAERIA EWFGRLVAHA VREPGTRVSD LPLIGAEERG
RLLALGDGGG LGPEPAGVLE RFARTVAAAR SGDIAVTAPD GSVSFAELDE RSDRIAALLE
DAGVGAGEPV AVLLPRSVDS IAALLGVLKA GAVYAPLDDS LPQGRIDAVL ADARPGAVLT
ADATAARVPD GYRRLDLGAA PTARLPEGTA RPGAGSPAYL LHTSGSTGRP KGVLVGHGSL
ARLLDHHRRR LFGPAVRETG RSRLQVALTA SLSFDAAWDP VLWMIDGHRL HVLDDLTRRD
PEALAEAVRT RGLDVLESTP THVRQLLDAG LFASPADAPA VLVLGGEAVP PSLWSALREQ
AGVRCLNFYG PTEATVDTLT ADVRDFTRPV LGTPVGGTRA YLLDARLRPV PVGVTGDLYL
AGDSPALGYH GRPAETARVF LPDPYGPAGA RMYRTGDRAV RVADGALEFA GRADGQVKVR
GFRVEPDEIT AVLESHPDVV HAAVVTRGDG PVGASLAAYV VTRPPYGATD PDGRTAALLA
ALRDHVAAHL PAYMVPSGWL AVDGIPLTPS GKLDRAALPE PLPVVSVAGA GRSPRNPRED
VLCTLFAEVL GVDKVLIDDD FFALGGHSLL ATRLIGRIRA TLGVDASIRS LFEAPTVAAL
AERLLDGVRD DPLATLLPLR TTGSRPPLFC VHPAGGLAWV YGGLLPHLDD DQPVYGLQTP
NLDGTAPFPD SIEDMAALYV AELRRVQPHG PYHLFGWSFG GNVVQEAAVQ LQEAGERVAL
LTILDAFPLA PLDDLDSAGR DTVFRALLDN LGVGGEALAG AEKVEAASVR EEFRRVGSPL
GSLEPATIDV MVDNFAGQAR LMRAYTPRTF HGPVLFFTAT EGRSRDTFPL GLWAPYIEGH
IENHDVACAH AQMMSPAARE QIGTTVSAAL RATHHIAQGD LS
//
