ID G2NBD6_STREK Unreviewed; 361 AA.
AC G2NBD6;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Peptidase alpha-lytic pro domain protein {ECO:0000313|EMBL:AEN09091.1};
DE Flags: Precursor;
GN ORFNames=SACTE_1168 {ECO:0000313|EMBL:AEN09091.1};
OS Streptomyces sp. (strain SirexAA-E / ActE).
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=862751 {ECO:0000313|EMBL:AEN09091.1, ECO:0000313|Proteomes:UP000001397};
RN [1] {ECO:0000313|EMBL:AEN09091.1, ECO:0000313|Proteomes:UP000001397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SirexAA-E / ActE {ECO:0000313|Proteomes:UP000001397};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Adams A., Raffa K.,
RA Adams S., Book A., Currie C., Woyke T.;
RT "Complete sequence of Streptomyces sp. SirexAA-E.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AEN09091.1, ECO:0000313|Proteomes:UP000001397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SirexAA-E / ActE {ECO:0000313|Proteomes:UP000001397};
RX PubMed=24710170; DOI=10.1371/journal.pone.0094166;
RA Takasuka T.E., Acheson J.F., Bianchetti C.M., Prom B.M., Bergeman L.F.,
RA Book A.J., Currie C.R., Fox B.G.;
RT "Biochemical properties and atomic resolution structure of a
RT proteolytically processed beta-mannanase from cellulolytic Streptomyces sp.
RT SirexAA-E.";
RL PLoS ONE 9:e94166-E94166(2014).
CC -!- SIMILARITY: Belongs to the peptidase S1 family.
CC {ECO:0000256|ARBA:ARBA00007664}.
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DR EMBL; CP002993; AEN09091.1; -; Genomic_DNA.
DR RefSeq; WP_014045080.1; NC_015953.1.
DR AlphaFoldDB; G2NBD6; -.
DR STRING; 862751.SACTE_1168; -.
DR MEROPS; S01.266; -.
DR KEGG; ssx:SACTE_1168; -.
DR PATRIC; fig|862751.12.peg.1222; -.
DR eggNOG; COG0265; Bacteria.
DR HOGENOM; CLU_030648_0_1_11; -.
DR OrthoDB; 8781117at2; -.
DR Proteomes; UP000001397; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR CDD; cd21112; alphaLP-like; 1.
DR Gene3D; 3.30.300.50; -; 1.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR InterPro; IPR004236; Pept_S1_alpha_lytic.
DR InterPro; IPR001316; Pept_S1A_streptogrisin.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR035070; Streptogrisin_prodomain.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF02983; Pro_Al_protease; 1.
DR Pfam; PF00089; Trypsin; 1.
DR PIRSF; PIRSF001134; Streptogrisin; 1.
DR PRINTS; PR00861; ALYTICPTASE.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR001134-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000001397};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..33
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 34..361
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003433948"
FT DOMAIN 102..157
FT /note="Peptidase S1A alpha-lytic prodomain"
FT /evidence="ECO:0000259|Pfam:PF02983"
FT DOMAIN 200..352
FT /note="Peptidase S1"
FT /evidence="ECO:0000259|Pfam:PF00089"
FT ACT_SITE 206
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001134-1"
FT ACT_SITE 235
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001134-1"
FT ACT_SITE 317
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001134-1"
FT DISULFID 187..207
FT /evidence="ECO:0000256|PIRSR:PIRSR001134-2"
FT DISULFID 311..338
FT /evidence="ECO:0000256|PIRSR:PIRSR001134-2"
SQ SEQUENCE 361 AA; 36755 MW; 0F38F7BCA6AD5282 CRC64;
MSHRRISRKR AALAGSAVVA LVVTGVSFQT ANASDDVPQF TARTLTAQAA GNLATTLDKT
LGADAAGAYY DAEAKHLVVN VLDEAAAEQV RQAGGTARIV QNSVAELKAA RQTLADRATV
PGTSWAVDPV SNKVVVTADR TVDSASWDKL GAVVKGLGGK AQLNRTAGEF KPLIAGGDAI
WGNGGRCSLG FNVVKDGEPY FLTAGHCTDA ITSWSETQTG AEIGANEDSS FPDNDYGLVK
YTSGTAHPSE VDLYNGSTQK ITKAGEATVG MTVTRSGSTT QVHDGEVTAL DATVNYGNGD
IVNGLIQTTV CAEPGDSGGS LFAGDTAIGL TSGGSGDCSA GGTTFFQPVP EALAAYGAEI
G
//
