ID G2T3Z4_ROSHA Unreviewed; 545 AA.
AC G2T3Z4;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=RHOM_10860 {ECO:0000313|EMBL:AEN97281.1};
OS Roseburia hominis (strain DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX NCBI_TaxID=585394 {ECO:0000313|EMBL:AEN97281.1, ECO:0000313|Proteomes:UP000008178};
RN [1] {ECO:0000313|EMBL:AEN97281.1, ECO:0000313|Proteomes:UP000008178}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183
RC {ECO:0000313|Proteomes:UP000008178};
RX PubMed=26543119; DOI=10.1128/genomeA.01286-15;
RA Travis A.J., Kelly D., Flint H.J., Aminov R.I.;
RT "Complete genome sequence of the human gut symbiont Roseburia hominis.";
RL Genome Announc. 3:E0128615-E0128615(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP003040; AEN97281.1; -; Genomic_DNA.
DR RefSeq; WP_014080299.1; NC_015977.1.
DR AlphaFoldDB; G2T3Z4; -.
DR STRING; 585394.RHOM_10860; -.
DR REBASE; 40180; M.Rho183ORF10860P.
DR GeneID; 77459755; -.
DR KEGG; rho:RHOM_10860; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_037306_0_0_9; -.
DR OrthoDB; 9815272at2; -.
DR BioCyc; RHOM585394:G1H02-2166-MONOMER; -.
DR Proteomes; UP000008178; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AEN97281.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008178};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 179..499
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 545 AA; 62302 MW; 593959937FA9B57E CRC64;
MTTEAIIANT KQMIDDLKTV CTNFGLGNAS SEYKIITEIF LYKFLNDKFI YEVQQADEEL
KNSENVEQAL NIMSEDDYEM LMMLLPPATA KLKREHFISY LFNHKNDEKF NELFDSTLWD
ISNTNLDVFS VSTGSGDKIR LFDQNLSQNV TESNRRSDFC KAMIDKLVTF SFAEAFSQKY
DFFATIFEYL IKDYNKDFGK YAEYYTPHSI ASIIARIMVP EGVQNVTVYD PAAGSGTLVL
ALAHEIGESN CTIYTQDISQ KSNEFLRLNL ILNNLVHSLG HVVHGDTLLS PQHLNRQKNG
LMKFDYIVSN PPFNVDFSDN RDTLAGDIYK ERFWAGVPNV PNKNKDSMAI YQMFLQHIIF
SMKENGGRAA VVVPTGFLTA GTRIPKKIRE RIVEDRMLRG VVSMPSNIFA TTGTNVSVLF
LDNSKKYEQA ILMDASKLGT KIKVDGKNQR TVLSPEEIEN IIDTFNNFET KDDFSVVVDY
DKIEQKKCSF SAGQYFEVKI EYVELTQEEF QKKMDGYTEK LTELFAEGNA LQTEILEQLK
KVKYE
//
