ID G2XV83_BOTF4 Unreviewed; 2545 AA.
AC G2XV83;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 76.
DE SubName: Full=BcPKS11, polyketide synthase {ECO:0000313|EMBL:CCD44403.1};
GN ORFNames=BofuT4_P059830.1 {ECO:0000313|EMBL:CCD44403.1};
OS Botryotinia fuckeliana (strain T4) (Noble rot fungus) (Botrytis cinerea).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Sclerotiniaceae; Botrytis.
OX NCBI_TaxID=999810 {ECO:0000313|EMBL:CCD44403.1, ECO:0000313|Proteomes:UP000008177};
RN [1] {ECO:0000313|Proteomes:UP000008177}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T4 {ECO:0000313|Proteomes:UP000008177};
RX PubMed=21876677; DOI=.1371/journal.pgen.1002230;
RA Amselem J., Cuomo C.A., van Kan J.A., Viaud M., Benito E.P., Couloux A.,
RA Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E.,
RA Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.M.,
RA Quevillon E., Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P.,
RA Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I.,
RA Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P.,
RA Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C.,
RA Grossetete S., Guldener U., Henrissat B., Howlett B.J., Kodira C.,
RA Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E.,
RA Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N.,
RA Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E.,
RA Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA Zeng Q., Rollins J.A., Lebrun M.H., Dickman M.;
RT "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT sclerotiorum and Botrytis cinerea.";
RL PLoS Genet. 7:E1002230-E1002230(2011).
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DR EMBL; FQ790270; CCD44403.1; -; Genomic_DNA.
DR STRING; 999810.G2XV83; -.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_31_1_1; -.
DR InParanoid; G2XV83; -.
DR Proteomes; UP000008177; Unplaced contigs.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd05195; enoyl_red; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775:SF29; ASPERFURANONE POLYKETIDE SYNTHASE AFOG-RELATED; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF13602; ADH_zinc_N_2; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 1.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008177};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 35..460
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2445..2522
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2545 AA; 277762 MW; 9F59ADEEA66BF43F CRC64;
MGSLGPTRVT NGNPVNNGSA AVNGDSPTAA HGGHLEPIAV VGMSCRLSGD ASDTQKLWEL
LIKGRSAWSK VPKDRFNQEA FHDPSAEGKP GRTSTDGGHF LKDDIKAFDA SFFGINPIEA
TAMDPQQRLL LEIAYEAFEN AGLTLEQLSG SNTGVYVGQW ASDYQEMLLR DIDFPPIYQA
SGVGAAISSN RISYCFNLHG PSLTLDTGCS ASLVALHQAV HSLRAGETDK CFVAGVNLQL
DPQRYGYQNK LSMFSKQGKS FTFDHRAKTA SGYGRGEGCS GVVLMPLSQA QKQGFPIRAV
VQNSVANQDG KTNGITVPSA EAQSAAIEKA YSQVGLVPYA DYVEAHGTGT AVGDPIEARA
IAKVLGAGRE SDSPLPIGSL KANIGHTESA AGLTGLIKAV LMLEHNMIPP QVNFEKANPE
IDLDALNLRI PITLESRPLK RISVNSFGYG GTNAHVVVDA AREIATVKPT SQNNGISTTY
DHHVPEDASR AKERLFVLSA ASEKSCQDMS SKVAKYLESK ADSPDADTLL SRLAYTLSRR
SVMANRVGVI ASDLNDLIAQ LTSLSTEAIP RADHQTTPRI GFVFSGQGAQ YPRMGQSLLG
TWPTFTASMK RAAACIKACG SSWDLLEELL KKPAESRMED PRIAQPISTA VQISLVDTLK
DLGVFPSAVI GHSSGEIAAA YCAEAISFED AMTVAYHRGR LTGDLRKENK GKAGAMIAVG
ASATTVKQSI DQLGAAVSSR IAIACFNSPE SVTVSGDEDV INSLKQRLDE RDIWNRILRT
GGAAYHSPQM LQVAEKYHEA LKDLSGGVPV SNISMVSSVT GEDHADKPIT RDYWVHNLVS
PVRFTDALKK TCVGESGTRK VDLLLELGSH FQLEGPIKQT LRTFTGQPAK IHYAGSLKRG
ENAQRRLLEM LRSLYLERFP VAFWKANAGF VETAPELLTD LPPYPFDHSK TYWHESRVSK
AYKHRTFLPH ELLGSLVHDN NPQEPRWRCF LNLKDVPWLS GHIIQGQTIF PATAYLAMVF
QAARQDTAMR NPQATINRFI LRNVNMSQAL VMENNKPDLE ISLSLRPQAV SARKSSQLWQ
EFRIFSTSAE DIWTEHCRGL VQVVLKSESA TQDDGIVPTR VTLPPHAHHI QAKQFYHRAR
DIGVNWSAPF DNLTDIKIAS GSCIADSSFV PGAGASETDY TIHPGVMDAV LYHGMMGVLI
FDENDKSPVV PTFIEKMIVA EQDRAKPVQE VTCHATRTDN ALTFDIGIFE KQNEENMVLQ
AWGVVATKLP DVSIGEGRKR DLCHVPDWVT YMPKTTQKHI DDLCKKSLEE RSIIPEIKAL
EALTVNYVKR ALASTPADEV AEGYQRNWFK WMKTLADQEP DPVFLKAGEE DGSVSAEVAR
RLGPRLSEIL RGTTHPLDIL NEDNLLSGLY LEGGNARCIA QIAAYMGELG RLNPNMKIIE
VGAGTGSATL PVLQALQAPN RVLASEYDFT DISPGFFPAA RELLSDFENI MQYKTLNVEK
TAEENGFEPN SYDVLIASNV LHATPCIDVV LENVRSLLRP GGKLILMEPT ENLPHYGLVF
GSLAGWWAGV DEGRTLSPML SRSAWIQKLS KNGFVHNEAV FEDYPVADGG SISVFVSEVP
VPAPSAAPLD VEIVSYSGDG VYSSFTEKLQ KNLWDRSVEA CVASPSVSED KISIIMPDIC
ERVAWNMDGP LFESIKARVA NSKVIVFVMC TAKGKEQRPS GDWIYGFCRS IRYEYVSVRL
VSLELESGLD AGVEALSTIL NSPTADMSLP IDEVELEYMD RDGQLYVSRV RSEPNLDSYV
SRDLGRSVSE EAPFFNDRAM SAELGIPGVL DTLRWADNPE ISGPVDPDCV RLQLSAASIN
FKDVLVASGQ LEGITQMRND CSGIVLEVGA NMTSKFKPGD RVCSLYSQSF TNYPMVHGLC
CHVIPQDMDF ADAASIPLVW CTVYYCLITI ARLQKGESIL IHSAAGAVGQ ASIMLARHLG
AEIFVTCGNE AKVELLNTEF NIPKDHIFSS RTTAFRDKIH AMTKGKGVDV VLNSLGGEMF
RESCNTLAAH GRFVEIGRKD LMENALMPME FLLNNITFTY VDFAHILATK TWLASQLLGD
VMKLFASGAV QHVRQIKYSI SEMAAAFRLI QAGKHTGKVV LTVDPEVKVQ VVPSKPTSVQ
LNPEATYIVV GGLGGLGKRL VDWTTEKGAR HICIFSRTAQ PDSDTQSFLD KLKSMGVKVR
VEKCDVSSEE SLKKAVAMIQ KTMPPIRGLF QAAMVLHDVL LEDMTVEQWC KVTAPKVQGT
WNLHKLLPDD MDFFIMLSSV VSMVGTVGAS NYASSCAFQD GIARYRRRLG LPAYAINIGA
IVEAGYVSEN PDVAVNLRKN GLGSVAISEF LSHLADVIQY KTEYTQSSLG ILPNGTERGL
GEARWSNDKR LAQIFGSESQ AGSKAVGSGA DNVGFVLQAA TTFHEAQDIV CKAIVKQLAT
ILAIDASDII PARSLDSYGL DSLVGVELRN WIGAYLQVNL PLLVMWNTSS INELAEIVTK
GSRLVKVKSE ETEEEKKDVE EAKEE
//
