ID G3C8Z4_PINPS Unreviewed; 523 AA.
AC G3C8Z4;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Serine hydroxymethyltransferase {ECO:0000256|RuleBase:RU000585};
DE EC=2.1.2.1 {ECO:0000256|RuleBase:RU000585};
GN Name=mshmt {ECO:0000313|EMBL:CCC55420.1};
OS Pinus pinaster (Maritime pine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC Pinus subgen. Pinus.
OX NCBI_TaxID=71647 {ECO:0000313|EMBL:CCC55420.1};
RN [1] {ECO:0000313|EMBL:CCC55420.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Differentiating xylem {ECO:0000313|EMBL:CCC55420.1};
RA Canton F.;
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CCC55420.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Differentiating xylem {ECO:0000313|EMBL:CCC55420.1};
RA Pacheco-Villalobos D., Diaz-Moreno S.M., Canas R.A., Said E.-.S., Osuna D.,
RA Van Kerckhoven S.H.E., Bautista R., Claros M.G., Canovas F.M., Canton F.R.;
RT "Comparative transcriptome analysis of compression and opposite wood
RT formation in maritime pine by suppression subtractive hybridization coupled
RT with microarray.";
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Interconversion of serine and glycine.
CC {ECO:0000256|RuleBase:RU000585}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =
CC (6S)-5,6,7,8-tetrahydrofolate + L-serine; Xref=Rhea:RHEA:15481,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:33384,
CC ChEBI:CHEBI:57305, ChEBI:CHEBI:57453; EC=2.1.2.1;
CC Evidence={ECO:0000256|RuleBase:RU000585};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|PIRSR:PIRSR000412-50, ECO:0000256|RuleBase:RU000585};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU000585}.
CC -!- SIMILARITY: Belongs to the SHMT family. {ECO:0000256|ARBA:ARBA00006376,
CC ECO:0000256|RuleBase:RU000585}.
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DR EMBL; HE574554; CCC55420.1; -; mRNA.
DR AlphaFoldDB; G3C8Z4; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0019264; P:glycine biosynthetic process from serine; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00378; SHMT; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR HAMAP; MF_00051; SHMT; 1.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR001085; Ser_HO-MeTrfase.
DR InterPro; IPR019798; Ser_HO-MeTrfase_PLP_BS.
DR InterPro; IPR039429; SHMT-like_dom.
DR PANTHER; PTHR11680; SERINE HYDROXYMETHYLTRANSFERASE; 1.
DR PANTHER; PTHR11680:SF64; SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF00464; SHMT; 1.
DR PIRSF; PIRSF000412; SHMT; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR PROSITE; PS00096; SHMT; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase {ECO:0000313|EMBL:CCC55420.1};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563,
KW ECO:0000256|RuleBase:RU000585};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW ECO:0000256|PIRSR:PIRSR000412-50};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000585}.
FT DOMAIN 60..457
FT /note="Serine hydroxymethyltransferase-like"
FT /evidence="ECO:0000259|Pfam:PF00464"
FT MOD_RES 291
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR000412-50"
SQ SEQUENCE 523 AA; 57832 MW; 1DFF8D744E22B1A0 CRC64;
MAMAMATMRK ISSSALFRPG SAMLQRALQS TVALSNEADY SRLEKQKSHV TWPKQLNAPL
EEVDPEIANI IELEKNRQWK GLELIPSENF TSLSVMQAVG SVMTNKYSEG YPGARYYGGN
EFIDMAESLC QKRALEAFGL DPDKWGVNVQ PLSGSPANFQ VYTALLKPHD RIMALDLPHG
GHLSHGYQTD TKKISAVSIF FETMPYRLDE STGYIDYDQL EKSAVLFRPK LIVAGASAYA
RHYDYARMRK VCDKQKAVLL ADMAHISGLV AAGVVPSPFE FADVVTTTTH KSLRGPRGAM
IFYRKGIKEI NKQGQEVKYD YEEKINAAVF PGLQGGPHNH TITGLAVALK QATTSEYKAY
QEQVLSNCAH FAKCLSERGY ELVSGGTDNH LVLVNLKNRG IDGSRVERVL ELVHIAANKN
TVPGDISAMV PGGIRMGTPA LTSRGFVEDD FAKVAEFFDL ATQLAIRIKS ETKGGSKLKD
FKATIESSPH FQAEIASLRH NVEEYAKQFP TIGFEKTSLK YKD
//
