ID G3HHV5_CRIGR Unreviewed; 787 AA.
AC G3HHV5;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=RAS guanyl-releasing protein 1 {ECO:0000256|ARBA:ARBA00041172};
DE Flags: Fragment;
GN ORFNames=I79_010216 {ECO:0000313|EMBL:EGW04307.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGW04307.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000256|ARBA:ARBA00004514}. Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004406}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004406}. Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004395}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004395}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the RASGRP family.
CC {ECO:0000256|ARBA:ARBA00009566}.
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DR EMBL; JH000389; EGW04307.1; -; Genomic_DNA.
DR AlphaFoldDB; G3HHV5; -.
DR STRING; 10029.G3HHV5; -.
DR PaxDb; 10029-XP_007632735-1; -.
DR eggNOG; KOG3417; Eukaryota.
DR InParanoid; G3HHV5; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd20860; C1_RASGRP1; 1.
DR CDD; cd22290; cc_RasGRP1_C; 1.
DR CDD; cd00051; EFh; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 3.30.60.20; -; 1.
DR Gene3D; 6.10.250.2730; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR046349; C1-like_sf.
DR InterPro; IPR020454; DAG/PE-bd.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR002219; PE/DAG-bd.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF174; RAS GUANYL-RELEASING PROTEIN 1; 1.
DR Pfam; PF00130; C1_1; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR PRINTS; PR00008; DAGPEDOMAIN.
DR SMART; SM00109; C1; 1.
DR SMART; SM00054; EFh; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF57889; Cysteine-rich domain; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 41..167
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 196..427
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT DOMAIN 461..496
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 532..582
FT /note="Phorbol-ester/DAG-type"
FT /evidence="ECO:0000259|PROSITE:PS50081"
FT COILED 731..772
FT /evidence="ECO:0000256|SAM:Coils"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EGW04307.1"
SQ SEQUENCE 787 AA; 89662 MW; C869975DEB41E466 CRC64;
KPPHGSRTGP KARLEAKSVN NPLPAQPSLA QITQFRMMVS LGHLAKGASL DDLIDSCIQS
FDADGNLCRS NQLLQVVLTM HRIIISSAEL LQKLMSLYPT YKDALEKNSP GICLKICYFV
RYWITEFWIM FKMDASLTST MEEFQDLVKN NGEESHCRLI DTTQINSRDW SRKLTQRIKS
NTSKKRKVSL LFDHLEPEEL SEHLTYLEFK SFRRISFSDY QNYLVNSCVK ENPTMERSIA
LCNGISQWVQ LMVLSRPTPQ LRAEVFIKFI HVAQKLHQLQ NFNTLMAVIG GLCHSSISRL
KETSSHVPHE INKVLGEMTE LLSSCRNYDN YRRAYGECTH FKIPILGVHL KDLISLYEAM
PDYLEDGKVN VQKLLALYNH INELVQLQEV APPLDANKDL VHLLTLSLDL YYTEDEIYEL
SYAREPRNHR APPLTPSKPP VVVDWASGVS PKPDPKTISK HVQRMVDSVF KNYDLDQDGY
ISQEEFEKIA ASFPFSFCVM DKDREGLISR DEITAYFMRA SSIYSKLGLG FPHNFQETTY
LKPTFCDNCA GFLWGVIKQG YRCKDCGMNC HKQCKDLVVF ECKKRIKSPA VSTENISSVV
PMSNLCPLGT KDLLHAPEEG SFIFQNGEAV DHSEESKDRT IMLLGVSSQK ISVRLKRTVA
HKTTQTESFP WVGGEVPAGH FVLSSPRKPP QDALFVLPSP TSPCPSPVLL RKRAFVKWEN
KESLIKPKQE LHLRLRTYQE LEQEINTLKA DNDALKIQLK YAQKKIESLQ LAKSNHVIAQ
MDRGDCS
//
