ID G4T013_META2 Unreviewed; 1722 AA.
AC G4T013;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN OrderedLocusNames=MEALZ_0606 {ECO:0000313|EMBL:CCE22301.1};
OS Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM
OS B-2133 / 20Z) (Methylomicrobium alcaliphilum).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylotuvimicrobium.
OX NCBI_TaxID=1091494 {ECO:0000313|EMBL:CCE22301.1, ECO:0000313|Proteomes:UP000008315};
RN [1] {ECO:0000313|Proteomes:UP000008315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z
RC {ECO:0000313|Proteomes:UP000008315};
RX PubMed=22207753; DOI=10.1128/JB.06392-11;
RA Vuilleumier S., Khmelenina V.N., Bringel F., Reshetnikov A.S., Lajus A.,
RA Mangenot S., Rouy Z., Op den Camp H.J., Jetten M.S., Dispirito A.A.,
RA Dunfield P., Klotz M.G., Semrau J.D., Stein L.Y., Barbe V., Medigue C.,
RA Trotsenko Y.A., Kalyuzhnaya M.G.;
RT "Genome sequence of the haloalkaliphilic methanotrophic bacterium
RT Methylomicrobium alcaliphilum 20Z.";
RL J. Bacteriol. 194:551-552(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
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DR EMBL; FO082060; CCE22301.1; -; Genomic_DNA.
DR RefSeq; WP_014147107.1; NC_016112.1.
DR STRING; 1091494.MEALZ_0606; -.
DR KEGG; mah:MEALZ_0606; -.
DR PATRIC; fig|271065.3.peg.620; -.
DR HOGENOM; CLU_002862_0_0_6; -.
DR Proteomes; UP000008315; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 5.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR InterPro; IPR027785; UvrD-like_helicase_C.
DR NCBIfam; TIGR00614; recQ_fam; 1.
DR PANTHER; PTHR13710; DNA HELICASE RECQ FAMILY MEMBER; 1.
DR Pfam; PF13245; AAA_19; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF13538; UvrD_C_2; 1.
DR SMART; SM00487; DEXDc; 2.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000008315}.
FT DOMAIN 300..480
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 506..674
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 1097..1315
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 1118..1125
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1722 AA; 192719 MW; 6DFAE10D122C16BA CRC64;
MTTDWQAFQN DCCSLDLETN ENGEIFALGG RFRGREFLRK APFDIRSVLA EFDRFAAEAG
CLLGHNLIGH DLPVCRAIAP NHAFLNKPVV DTLYLSPLAF PENPYHRLVK NYKLVRDGLS
DPLLDAKLAE SLFQDQWSAL HEQQQAFGIV SFYHYAFSGD PGFSGICRMM EAVGAVLPGA
SQAFDIFKQT SSGKVCETAF NKVVLSYLPD PRLRQALAYC LAWLRVAGGN SVLPPWVRQR
FHDVGPALSQ LRDIPCGNPA CRYCAGVHDP VSQLTRYFGF PAFREEPASD DGGSLQQAIV
QAAMSDRPLF AVLPTGGGKS LCFQLPALVR YQRRGVLTVV ISPLQALMKD QVDNLRNKTG
APNVAALYGL LTAPERGEVL RAIQMGDIAI LYVSPEQLRN TGFQNAIAQR EIGCWVFDEA
HCLSKWGHDF RPDYLYAARF IKEFAAKHRA PLPPVQCFTA TAKQDVKDEI IDYFRAHLGQ
ELEVFEGGVE RSNLHFEVQT VNAADKYPRI HALLTERLTG ETGSAIVYCS TQKNTEAVAE
FLQQAGWQAE AFHAGKDAAE KKHIQENFVG GITRVISATN AFGMGIDKED VRLVIHADIP
GSLENYLQEA GRAGRDRQDA ECVLLFDEND IDTQFKLSAH SQITQRDIAQ LLRGIRRCKK
DKFGNVILTA GELLQNDAVD TSFGSDDYNA ATKVVTAVSW LERAGFVERN ENRTQVFQGR
PKVKSLDEAE AKIDKLPLSQ RQKKRWLAIL EELFNADSDE GFSADQLAML GAFAENDEDK
PGETASQRVI RTLYDMAEQG LIQKSLLLTA FIRYKVAQSS QAQLTAVCKL ERAMIAAMGE
LAPDAAGDDW QVLSLRHLNQ HLLDQGYEQS HLEALRLLLN SLSRDGLGLA GNKGSLFVRH
RGLDQYAVKL NRGWPALIAT AEKRQGIAKI VLDCIVGRVP EGAPPAADVL VEFAAEDLLS
ALRQDAVALA EVKDPLAAVE RALNFLHEQK IITLQKGLAV FRQAMTINVL PEAKSRRYRK
GDYEPLSQHY GERVFQVHVM NEYARRGLEK IGQALAFVVA YFSLDKAEFV QRYFAGRKEI
LERAASQQAF QRIASDLNNP EQIALVSAPE DDNALILAGP GSGKTRVVVH RCAYLLRVKQ
VLAQAILVLC FNRSAANEIR RRLIELAGDE ARYVTVQTYH GFSLRLTGHA LSGLYGDGEA
SAESFAEMIG EAIALLKGEK NLLGVEPDAA RDRLLAGYRF ILVDEYQDID AEQYRLISAI
AGRSLDEDSK LTILAVGDDD QNIYQFRGAN VEFIRQFQQD YQAHVHYLVE NYRSSAHIIA
AANALIGHNR DRMKQQHPIR INKQRGNLEP GGRWQALDTL TRGRVRQIDT VGESMQALQV
AEELKQMRQL DVRLQWPNCA VLAREWRYLS PVRSVFEAQG IPVSIVLPAD RRPPPFRIRE
NAGLLDALKN HAEPLTRPSY WLDWLGEHYG EQPRNPWLMQ LKELLGDWRS ETGDGETANS
QTLDFIVETL AEQRLDRTFG QGVLLGTVHS VKGLEFDHVV IIDGGWNSRA GEEEQRRLLY
VAMTRARETL CLMHRKDLRN PYLSEIRGDH LLMTEVPELD AQDRPIILRD YEILGLKDFN
IGYAGSFPEQ HSIHRRLAEL KPGSELNFAR INGKLVLQSG GETVAALSQK AQHEWADKYV
NAEKTTVLAM IRRHAEDGEE QYRKRCQCSV WEVPFVEVVC RG
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