ID G4TRI1_SERID Unreviewed; 1011 AA.
AC G4TRI1;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE SubName: Full=Probable MIS1-C1-tetrahydrofolate synthase, mitochondrial {ECO:0000313|EMBL:CCA73924.1};
GN ORFNames=PIIN_07877 {ECO:0000313|EMBL:CCA73924.1};
OS Serendipita indica (strain DSM 11827) (Root endophyte fungus)
OS (Piriformospora indica).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Sebacinales; Serendipitaceae; Serendipita.
OX NCBI_TaxID=1109443 {ECO:0000313|EMBL:CCA73924.1, ECO:0000313|Proteomes:UP000007148};
RN [1] {ECO:0000313|EMBL:CCA73924.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11827 {ECO:0000313|EMBL:CCA73924.1};
RX PubMed=22022265; DOI=10.1371/journal.ppat.1002290;
RA Zuccaro A., Lahrmann U., Guldener U., Langen G., Pfiffi S., Biedenkopf D.,
RA Wong P., Samans B., Grimm C., Basiewicz M., Murat C., Martin F.,
RA Kogel K.H.;
RT "Endophytic Life Strategies Decoded by Genome and Transcriptome Analyses of
RT the Mutualistic Root Symbiont Piriformospora indica.";
RL PLoS Pathog. 7:e1002290-e1002290(2011).
RN [2] {ECO:0000313|EMBL:CCA73924.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 11827 {ECO:0000313|EMBL:CCA73924.1};
RA MIPS;
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the
CC formate--tetrahydrofolate ligase family.
CC {ECO:0000256|ARBA:ARBA00006985}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate
CC dehydrogenase/cyclohydrolase family. {ECO:0000256|ARBA:ARBA00005559}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCA73924.1}.
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DR EMBL; CAFZ01000264; CCA73924.1; -; Genomic_DNA.
DR AlphaFoldDB; G4TRI1; -.
DR STRING; 1109443.G4TRI1; -.
DR eggNOG; KOG4230; Eukaryota.
DR HOGENOM; CLU_003601_2_0_1; -.
DR InParanoid; G4TRI1; -.
DR OMA; QPIMFRR; -.
DR OrthoDB; 651667at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000007148; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR CDD; cd01080; NAD_bind_m-THF_DH_Cyclohyd; 1.
DR Gene3D; 1.10.8.770; -; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_01543; FTHFS; 1.
DR HAMAP; MF_01576; THF_DHG_CYH; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000672; THF_DH/CycHdrlase.
DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom.
DR InterPro; IPR020867; THF_DH/CycHdrlase_CS.
DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR PANTHER; PTHR48099:SF5; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR Pfam; PF01268; FTHFS; 1.
DR Pfam; PF00763; THF_DHG_CYH; 1.
DR Pfam; PF02882; THF_DHG_CYH_C; 1.
DR PRINTS; PR00085; THFDHDRGNASE.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00767; THF_DHG_CYH_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000007148}.
FT DOMAIN 76..196
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT catalytic"
FT /evidence="ECO:0000259|Pfam:PF00763"
FT DOMAIN 199..366
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF02882"
SQ SEQUENCE 1011 AA; 108781 MW; AD8159AD8D539DF4 CRC64;
MLTLLSLTNS SWVIVRPLYL PSKHAAVLAA AAAAQTIQDD YPLQQFQLGS EEDAYEGDRI
VEKVVHDMSD SQAKIIDGTA IAKEIRTSVR DRIEALKKTN PTFPTPKLVI IQAGERPDST
TYVKMKAKAC AEVGIDYAHV AMPADATAAQ IIEEVQKLNH DSSVHGILVQ MPLGENVGLE
GERQVMESVS PMKDVDGFHA YNIGHLSSRS SEPLFTPCTP SGVIHLLTTT GFNLAGSNVV
VLGRSDIVGN PVAAMLRKKD ATVTQCHSRT PKETMINALR NADVVVSAIG KPEYVKAEWL
KPGTIVIDVG TNYIPDSTKK SGQRLVGDVE FSQASGVASH ITPVPGGVGP MTVAMLISNT
LKSAERLWEK ERARKLVPLK LNVLEKVPRY ALVMDQRQFL TFHSDIEIAV AQTPKPITHL
ATEIGIRPDE LESYGKYKAK VELSILDRLA KRKDGKYIVI SGITPTPLGE GKSTTTIGLA
QAIGAHLGRP AFACVRQPSQ GPTFGIKGGA AGGGYSQVIP MDEFNLHLTG DIHAVSAANN
LLAAALDARM FHESTQTDKA LYNRLVPTVK GKRAFAPLMF KRLKKLGIDK TDPNELTPEE
INRFARLDVD PATITWNRVV DTNDRFLRKI VVGKGEAEKG FELTDLKDMR ERLGAMVVAT
SKRGEPITAD DIGVGGALAV LMKDAIKPNL MQTLEGTPVF VHAGPFANIA HGNSSILADR
IALKLAGTEP GETDSTREGY VLTEGGFGAD MGMEKFCNIK CRVSGLTPDA VVIVATTRAL
KMHGGGPEVT PGKPLADTYT KEDLETLGVG IGNLERHIEN AKKFGLKVIV GVNQFSSDSS
AELELICSRA MQAGADAAVV SNHWALGGAG AKDLAEAVVA ACEGESTFKF LYPLDIPIVD
KISAIAKEIY RADGIELSEL ARSQVELYEK QGYGNLPICM AKTQYSFSHD PKLKGTPTGF
TVPIRAVRLS AGAGFLYPLL GDMQTMPGLS TRPGFWEVGL DEETGRVVGL F
//
