ID G4WWA9_9POTY Unreviewed; 3148 AA.
AC G4WWA9;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE SubName: Full=Gp1 {ECO:0000313|EMBL:AEP81236.1};
OS Cucumber vein yellowing virus.
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC Patatavirales; Potyviridae; Ipomovirus.
OX NCBI_TaxID=137475 {ECO:0000313|EMBL:AEP81236.1};
RN [1] {ECO:0000313|EMBL:AEP81236.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Jordanian {ECO:0000313|EMBL:AEP81236.1};
RX PubMed=22383057; DOI=10.1007/s00705-012-1264-4;
RA Galipienso L., Rubio L., Aramburu J., Velasco L., Janssen D.;
RT "Complete nucleotide sequence of a severe isolate of cucumber vein
RT yellowing virus from Jordan.";
RL Arch. Virol. 157:1189-1192(2012).
CC -!- FUNCTION: An RNA-dependent RNA polymerase that plays an essential role
CC in the virus replication. {ECO:0000256|ARBA:ARBA00029404}.
CC -!- FUNCTION: Has RNA-binding and proteolytic activities.
CC {ECO:0000256|ARBA:ARBA00029399}.
CC -!- FUNCTION: Indispensable for virus replication.
CC {ECO:0000256|ARBA:ARBA00034080}.
CC -!- FUNCTION: Mediates the cap-independent, EIF4E-dependent translation of
CC viral genomic RNAs (By similarity). Binds to the cap-binding site of
CC host EIF4E and thus interferes with the host EIF4E-dependent mRNA
CC export and translation (By similarity). VPg-RNA directly binds EIF4E
CC and is a template for transcription (By similarity). Also forms
CC trimeric complexes with EIF4E-EIF4G, which are templates for
CC translation. {ECO:0000256|ARBA:ARBA00037225}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes glutaminyl bonds, and activity is further
CC restricted by preferences for the amino acids in P6 - P1' that vary
CC with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or
CC Gly) for the enzyme from tobacco etch virus. The natural substrate is
CC the viral polyprotein, but other proteins and oligopeptides
CC containing the appropriate consensus sequence are also cleaved.;
CC EC=3.4.22.44; Evidence={ECO:0000256|ARBA:ARBA00000785};
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000256|ARBA:ARBA00004541}. Host cytoplasmic vesicle
CC {ECO:0000256|ARBA:ARBA00034108}. Vesicle
CC {ECO:0000256|ARBA:ARBA00004373}. Virion
CC {ECO:0000256|ARBA:ARBA00004328}.
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DR EMBL; JF460793; AEP81236.1; -; Genomic_RNA.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0044161; C:host cell cytoplasmic vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0031982; C:vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23175; ps-ssRNAv_Potyviridae_RdRp; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR022199; DUF3725.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002540; Pept_S30_P1_potyvir.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001592; Poty_coat.
DR InterPro; IPR001730; Potyv_NIa-pro_dom.
DR InterPro; IPR013648; PP_Potyviridae.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF83; PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF12523; DUF3725; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00863; Peptidase_C4; 1.
DR Pfam; PF01577; Peptidase_S30; 1.
DR Pfam; PF00767; Poty_coat; 1.
DR Pfam; PF08440; Poty_PP; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR PRINTS; PR00966; NIAPOTYPTASE.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51436; POTYVIRUS_NIA_PRO; 1.
DR PROSITE; PS51871; PV_P1_PRO; 2.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Covalent protein-RNA linkage {ECO:0000256|ARBA:ARBA00022520};
KW Helical capsid protein {ECO:0000256|ARBA:ARBA00022497};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Host cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00022488};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protease {ECO:0000256|ARBA:ARBA00022825};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Virion {ECO:0000256|ARBA:ARBA00022844}.
FT TRANSMEM 1091..1114
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 393..525
FT /note="Peptidase S30"
FT /evidence="ECO:0000259|PROSITE:PS51871"
FT DOMAIN 690..843
FT /note="Peptidase S30"
FT /evidence="ECO:0000259|PROSITE:PS51871"
FT DOMAIN 1253..1404
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 1423..1583
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 2049..2272
FT /note="Peptidase C4"
FT /evidence="ECO:0000259|PROSITE:PS51436"
FT DOMAIN 2525..2648
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT REGION 2848..2895
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2854..2895
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3148 AA; 360612 MW; 7F7619AD9B8E30A4 CRC64;
MTEVYSFVPK TDYEKRMVKR FGTDKFLQFR EQCPLNQVRK CWTHFKVCDG ACYAYLPVHF
AEEEAREFLE LSEGRRKLVL DNVRRALCSA LYYNPNDEDF ECECGNSNTS WKRVCEDCGQ
VYAYSEMNLL QELSRIAATL SCNINELKNF TIHDIADEVS YAERSRRMTT VESGFKTVDE
ISLRTEVVGA VISEKPVEPV TVKAVVAEKP VEASNNSRVA MEEKQPQIKQ VWRMVTEKPK
PTVVPECKLE APTKKEIQTC LDLVVQIGDF IINTCTREFK MVDGKSERIE KVEETEVKES
VVENSNKDTV VLDNFFKNFQ FNEERRSKIY TDKHGNVRYG RRPVKRQKRS KKVELMSDKI
ITKIEIPEEV QPVYSFEDLP SIKKAYSKEK PREIIETRTK YSVKSLVKEI GKTCSLTEIM
IIDKKVRKIK RRGSRYFVDV RHLDGCNPEV DLDHSRFSDE ILDWLMASLA VKSTRLSEIV
PGTSGLITAG RNEFGKFTII RGWLDRMVDA REKLTKSQLR RIRNYTIHGL HAFKKRYQTN
VIDRERTTQI QIKDNVVYLK GEGALQNFIN SSTLGGLVRN VSGATKPALA CLRKATKYGV
GFDMMMNHYV CCRCHVRCTD ISLMDPMCSE CGENMFESVG HESPFVTLPI NPFECDVHPA
VAEIMKEAWW RDGVEDWPIE KKYHSNGKEI YVEKQGQRAW RSEFQLRETS SNREFDLGLA
AANNGVCLCE ANFAWLDMFP CVTLNHIFGL DFDDDMDSEI RDIMTRENAQ RVFSTGRLVK
YADICPGWSG VVITEDAVQP EEWNKFAWYN GICVVQGKNR ESGIIENAII MKTADEKDKI
DFYSFDLSWA KSKTKFIEYF AEDETRIIKA CCTPSSLWLF AKKSSLYKYV DYLLLHESSI
VDLCVKFEFV GKHLSLLENV EDACIEFSHF MDEMIQSRNV QDEPELARIR ALIRSQFDSV
RESSKYEIID RIIEKKTKLQ ADEIIMRELI RRQYAELFSW RERALMNFCS KRTRLYDLWE
KREEANSSSS SIISLLMSRP GLEIMTNWAS TVCKTQYARS IRAVDCTIRF VWHRIVSLSK
QALFKWWESC VFHVFCVLLT ALVTYSLSQV VSFLKKMTDK EKKEALELEG DLVEIQGKKE
EATIMKWCAM LTLVMSFINF DWALASVSAL GKMKTIFSAL GPNLIELQSG EEDAFKFTTF
EVEVPGNGKS CDEQTFEDWI SHCIKYNLTT PEPTTSGPML VLEKGKAKEL AEKIRMHDSC
DLRVYGGVGT GKSTSLPSEI MKFGPVLICV PTRVLANALH DSYMALFGYD VSVAYRGRVR
TGTQPITVMT YGYALNHFHY NPQNLNSFEY VVMDEIHTFP TELNPLFSLI RETNPRKKII
KTSATHVGHN VELSTNFKVN IETLSPMGVK KWVELQGTNV FGDATSSGEV ILVFVATYNE
VDEAAEGLRN KGFPVLKVDG RNFRKNTEVQ KMVDDLPGTI KFIVATNIIE NGVTLDVDTL
VDFGERVSPV LDSDGRSIIM ARRRISKAER QQRFGRVGRM KPGTIYKFGK EALPDSMKSV
VGATESAMIC FAYGIKPVVD DVDIGSIARV TKKQALTASL YDLNRIFTVH HVDKHGFIPR
TVHELFKTFM LRTEAVAICE SYLSSDSSSW KALHTYLRPS EEMAHVRNVK IPWYCSDMSN
EFIIKLAECV NHAKPKFSCG YDVENVDFHV VAHKISVGEH NIEESKALVS EILNRVKQWR
DNLVYKMSTP RNNSLMSLMV GWIPKKIEKT RSXLEMRIQR LEMLLSQLDN VSVNHDYESL
VRFFAENPHS AEYLESQGKS EYLEKRILKT NIKEADWRVI AGVITITTCM AGFTYWYLRR
RSAVDQVEIQ GKVGYRRDKR VGRFVFDGPD EDIIENFGVE YSHDVVTKKM SKAQKLKQAK
ERGWKIGKVD RPKKIFKQLY GVNPLEFDEV YLTVGDFKGE IWETKDMDID EMYSDLYSDF
NLGNRKGYSK DVYLVFSKKD SDIEAVVDLQ PHRSKMASSM SLNPMGFPEE EGRWRQSGDV
KMRKRIEEEV EVQVAETAIA SKFAHIFQRL GRLGFSGRGL NCIFHGDKSI MPYHLASGGD
PGDSLIVTTS RGQFDMGPME MIKCKKITDF DLVVGQLPKD MQPFKSTNIM RKPKMDEDVV
IITLKRDKGK MLIRTSDTSK IYKAGDKYAH LWVHFIKSES GDCGSPIVAL SDNKIVGFHS
GMIKDKLGVF LRSVFTPVND ELLRTLNEKS EMNDFWKFNQ DAISWNAVIK TSSLFPVMKD
ILGVHLQVGA GDKYIGGNLM TVGEVNKHAY HNHVIKGKRK EFVEFCAQNP NNNFEKFRDF
YGPSVMTVSA FYKDLLKYDE PIRVGVIDFP SLVHAYLNVE DKLLSLGFDE DCGPEWDPYE
IYCDLNKKAA MGALYQGSKN EWLKEITPKE FIEQVQESYR LLGHGVVGIW SGSLKAELRT
KAKIAEGKTR VFTGAPIDVL LAGKVLVDKF NKHFYTQHLK GPWSVGINKF NRGWDKLARY
FNHDWKFIDC DGSRFDSSLS PILFQMVCHM RERFGHFDWA ETNALRNLYA QIVYTPILTI
DGNIVKKHKG NNSGQPSTVV DNTLILMLVV EYCKSWHLKQ SGIEMEFKYM CNGDDLIINA
PDKEISIIQS TFKNLFKECG LNYDFDDLHD SIEDVEYMSH HFVLREGFYI PKLSKERLVA
ILEWERSDEL FRTRSALNAA YIESFGYDDI HWEIERFAAY WANLKGVKNV LMSEDHVRKL
YLDENFELTD EIVQTLSPAS FEFGCVELQA DEIDKEAIEQ EIEKLRNEWK ANGPSRTVSN
YEARKKQTPI AAKVDELLKQ LKEAGVETLK RPCGQPNADE DKKESSNSNW TGESEDEDEE
KKKKMPLRGG GKILKRDDVD KIPTNAMEFK RDFKPARASR TSYIWIPRSQ RDNLTPDVIK
NFLAYIPPSQ AIDNQMASGS QVENWAMRTA SAYGVTIQQF YETVLPAWIV NCIVNGTSDE
RKTETVWRAV ELNAQGEDVD DMEYPIEPIY KHALPTMRKI MRNFSSQAIL MYQNSVAEGK
AFTVKAARNA GYTEIEDQWL GIDFLAEAQL SRNQLNIKHQ TLAANVSRNR RNLFALAAPG
DDGRVNAERH LTTDASASRH TYGGAMIE
//
