ID G4Z255_PHYSP Unreviewed; 673 AA.
AC G4Z255;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=PHYSODRAFT_491598 {ECO:0000313|EMBL:EGZ21390.1};
OS Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS (Phytophthora megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1094619 {ECO:0000313|EMBL:EGZ21390.1, ECO:0000313|Proteomes:UP000002640};
RN [1] {ECO:0000313|EMBL:EGZ21390.1, ECO:0000313|Proteomes:UP000002640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6497 {ECO:0000313|EMBL:EGZ21390.1,
RC ECO:0000313|Proteomes:UP000002640};
RX PubMed=16946064; DOI=10.1126/science.1128796;
RA Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H., Aerts A.,
RA Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA Damasceno C.M., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.K.,
RA McDonald W.H., Medina M., Meijer H.J., Nordberg E.K., Maclean D.J.,
RA Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA Rose J.K., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA Smith B.M., Sobral B.W., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT of pathogenesis.";
RL Science 313:1261-1266(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; JH159153; EGZ21390.1; -; Genomic_DNA.
DR RefSeq; XP_009524107.1; XM_009525812.1.
DR AlphaFoldDB; G4Z255; -.
DR STRING; 1094619.G4Z255; -.
DR EnsemblProtists; EGZ21390; EGZ21390; PHYSODRAFT_491598.
DR GeneID; 20656726; -.
DR KEGG; psoj:PHYSODRAFT_491598; -.
DR InParanoid; G4Z255; -.
DR OMA; MEYINLR; -.
DR Proteomes; UP000002640; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002640};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 340..673
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 146..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 640
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 673 AA; 76256 MW; 119E32BA7B2950E2 CRC64;
MLLCGQEAAA SEPARRRLAD SSDSYNFMFY FVIGAMMLTC LIYNIFFKHK MEANFLRQQA
LPETMLRQDV IASDQDMNNL WDCDVCGFKS YDAHTTCMLC GTDRAFKLLD KKDDANSAEL
GGIAPSSASS SAKYVLYMED KENPLSRSAV NSRATPPRPG RSKSGAKINR EFFKRKLSAL
NLRQQSARRR HEWSRERNDD GELIWVRKKH YMDRKVLKSL RTLDGLRSQS STSLRDSILS
TGSVNSVGIV SRIVVSPKNP TGRLSLETAE GALATRCSDS SEFSQDELEQ VGSLPFQQKH
AWFVQHTAAM EVPWEYGHLL LEIERDNLLH NSCEQLLWAT PDQLHQSLRI KFANEPGVDA
GGLVREWFTL MTKEVFDDTT GLFYRSGNGD GLMINPASAE ASVDHLMYYQ AIGRFIGRAL
FEGILIDAHL ALPVCKHILG IPITFSDLEF VDNDLYKNLK WLRDNTGVES LALDFTVNVD
QTSKVVDLVP NGSNIPVTEE NKMEYINLRF RWIVATSISQ QLGSLMQGLF SIIPKELLSV
FDHQELELLI CGIPDIDVLD WRTHTIYVGE RDERAITWFW NIVNEFTNEQ KARLLQFTTG
SARVPVQGFK ALTMNDGRIC PFAIQCVSVD ECLYPRAHTC FNRIDLPRYE AEKDLRIALS
LVIQMEVTGF TIE
//
