ID G5AZH7_HETGA Unreviewed; 4833 AA.
AC G5AZH7;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=GW7_18830 {ECO:0000313|EMBL:EHB02438.1};
OS Heterocephalus glaber (Naked mole rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Bathyergidae;
OC Heterocephalus.
OX NCBI_TaxID=10181 {ECO:0000313|EMBL:EHB02438.1, ECO:0000313|Proteomes:UP000006813};
RN [1] {ECO:0000313|EMBL:EHB02438.1, ECO:0000313|Proteomes:UP000006813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993625; DOI=10.1038/nature10533;
RA Kim E.B., Fang X., Fushan A.A., Huang Z., Lobanov A.V., Han L.,
RA Marino S.M., Sun X., Turanov A.A., Yang P., Yim S.H., Zhao X.,
RA Kasaikina M.V., Stoletzki N., Peng C., Polak P., Xiong Z., Kiezun A.,
RA Zhu Y., Chen Y., Kryukov G.V., Zhang Q., Peshkin L., Yang L., Bronson R.T.,
RA Buffenstein R., Wang B., Han C., Li Q., Chen L., Zhao W., Sunyaev S.R.,
RA Park T.J., Zhang G., Wang J., Gladyshev V.N.;
RT "Genome sequencing reveals insights into physiology and longevity of the
RT naked mole rat.";
RL Nature 479:223-227(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; JH167620; EHB02438.1; -; Genomic_DNA.
DR STRING; 10181.G5AZH7; -.
DR eggNOG; KOG1426; Eukaryota.
DR InParanoid; G5AZH7; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000006813; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd08664; APC10-HERC2; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14402; UBA_HERC2; 1.
DR CDD; cd02344; ZZ_HERC2; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 3.30.60.90; -; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 3.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR006624; Beta-propeller_rpt_TECPR.
DR InterPro; IPR021097; CPH_domain.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR037976; HERC2_APC10.
DR InterPro; IPR010606; Mib_Herc2.
DR InterPro; IPR037252; Mib_Herc2_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR InterPro; IPR041987; ZZ_HERC2.
DR PANTHER; PTHR22870; REGULATOR OF CHROMOSOME CONDENSATION; 1.
DR PANTHER; PTHR22870:SF437; X-LINKED RETINITIS PIGMENTOSA GTPASE REGULATOR-RELATED; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF11515; Cul7; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF06701; MIB_HERC2; 1.
DR Pfam; PF00415; RCC1; 17.
DR Pfam; PF00569; ZZ; 1.
DR PRINTS; PR00633; RCCNDNSATION.
DR SMART; SM01337; APC10; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00706; TECPR; 5.
DR SMART; SM00291; ZnF_ZZ; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF159034; Mib/herc2 domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 4.
DR SUPFAM; SSF63748; Tudor/PWWP/MBT; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS51416; MIB_HERC2; 1.
DR PROSITE; PS00626; RCC1_2; 1.
DR PROSITE; PS50012; RCC1_3; 18.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000006813};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00228}.
FT REPEAT 515..570
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 571..622
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 625..676
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 677..728
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 1208..1284
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
FT DOMAIN 1862..1935
FT /note="MIB/HERC2"
FT /evidence="ECO:0000259|PROSITE:PS51416"
FT DOMAIN 2706..2758
FT /note="ZZ-type"
FT /evidence="ECO:0000259|PROSITE:PS50135"
FT DOMAIN 2762..2939
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT REPEAT 2962..3013
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3014..3068
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3069..3120
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3121..3172
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3175..3226
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3227..3278
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3279..3330
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3956..4007
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4008..4061
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4062..4113
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4114..4165
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4168..4219
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4220..4271
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4272..4323
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 4461..4793
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 51..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2353..2373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3475..3503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3606..3633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4803..4833
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3482..3498
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4807..4821
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4766
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4833 AA; 525968 MW; 60EA4D349A28D32E CRC64;
MPSESFCLAA QARLDSKWLK TDIQLAFMRD GLCGLWNEMV KDGEIVYTGS ELSQNGELPP
RKDDGVDTQS GTKREDLNDK EKKDEEEAPA PAFRAKAILE SWVWGKQPDV TELKECLSVL
VKEQQALAVQ SATTTLSALR LRQRLVILER YFIALNRTVF QENVTVKWKS GSVSQPPMDK
KSSRPTGRGV EGLARVGSRA ALSFAFAFLR RAWRSGEDAD LCSELLQESL DALRALPEAS
LFDESSVSSV WLEVVERATR FLRSVVTGDV HGTPGTKGPG GVPLQDQHLA LAILLELAVQ
RGTLSQMLSA ILLLLQLWDS GAQETDNERS AQGTSAPLLP LLQRFQSITG SKDTPQAEGD
THLLSGPLSP NESFLRYLTL PQDNELAIDL RQTAVVVLAH LDRLASPCMP PLCSSPTSHK
GSLQEVIGWG LIGWKYYANV IGPIQCEGLA NLGVTQIACA EKRFLILSRN GRVYTQAYNS
DTLAPQLVQG LASRNIVKIA AHSDGHHYLA LAATGEVFSW GCGDGGRLGH GDTVPLEEPK
VISAFSGKQG GKHVVHIACG STYSAAITAE GELYTWGRGN YGRLGHGSSE DEAIPMLVAG
LKGLKVIDVA CGSGDAQTLA VTENGQVWSW GDGDYGKLGR GGSDGCKTPK LIEKLQDLEV
VKVRCGSQFS VALTKDGQVY SWGKGDNQRL GHGTEEHVRY PRLLEGLHGK KVIDVAAGST
HCLALTEDSE VHSWGSNDQC QHFDTLRVTK PEPAALPGLD TKHIVGIACG PAQSFAWSSC
SEWSIGLRVP FVVDICSMTF EQLDLLLRQV TEGMDGAADW PPPQEKECMA VATLNLLRLQ
LHAAISHQVD PEFLGLGLGS VLLNSLKQTV VTLASSVGVL STVQSAAQAV LQSGWSVLLP
TAEERARALS ALLPCAVSGN EVNISPGRRF MIDLLVGSLM ADGGLESALN AAISAEIQDI
EAKNEAQKEK EIDEQEANAS TFHRSRTPLD KDLINTGIYE SSGKQCLPLV QLIQQLLRNI
ASQTVARLKD VARRISSCLD FEQHNCERSA SLDLLLRFQR LLISKLYPGE SIGQTSDISS
PELMGVGSLL KKYTALLCTH IGDILPVAAS VASVSWRHFA EVACIVEGDF TGVLLPELVV
SIVLLLSKNP GLMQDAGAVP LLGGLLEHLD RFNHLAPGKE RDDHEELAWP GIMESFFTGQ
NCRNNEEVTL IRKADLENHN KDGGFWTVID GKVYDIKDFQ TQSLTGNSIL AQFAGEDPVV
ALEAALQFED TQESMHAFCV GQYLEPDQEV VTIPDLGSLS SPLIDTERNL GLLLGLHASY
LAVSTPLSPV EIECAKWLQL SIFSGGLQTS QIHYSYNEEK DEDHCSSPGG TPASKSRLCS
HRRALGDHSQ AFLQAIADNN IQDHNVKDFL CQIERYCRQS HLTTPIIFPP EHPVEEVGRL
LLCCLLKHED LGHVALSLVH AGTLGIEQVK HRTLPKAVVD VCRVIYQAKC SLIKTHQEQG
RSYKEVCAPV IERLRFLFNE LRPAVCNDLS IMSKFKLLSS LPRWRRIAQK IIRERRRNRD
IVPKKPESTD DEEKIGNEDS DLEEACILPH GPINVDKRPI TIKSPKDKWQ PLLSTVAGVH
KYKWLKQNVQ GLYPQSALHS TIAEFALKEE PVDVEKMRKC LLKQLERAEV RLEGIDTILK
LAAKNFLLPS VQYAMFCGWQ RLIPEGIDTG APLTDCLKDV DLIPPFNRML LEVTFGKLYA
WAVHNIRNVL MDANAKFKEL GIQPVPLQTI TNENSSGPSL GTIPQARFLL VMLSMLTVQH
GANNLDLLLN SGMLALTQTA LRLIGPSSDN MEEDTNASAQ GASATVLEET RKETAPVQLP
VSGPELAAMM KIGTRVMRGV DWKWGDQDGP PPGLGRVIGE LGEDGWIRVQ WDTGSTNSYR
MGKEGKYDLK LAELPASAQP LAEDSDTDDD SEAEQIEKNI HPTAMMLTST VNLLQTLCLS
VGVHAEIMQS EATKTLCGLL RMLVESGTTD KTSSPSRLVC RGQHRSWCTL GFVRSIALTP
QVCGALSSPR WIGLLMKVVE GHTPSTATSL QRQILAVHLL QAVLPSWDKT ERARDMKCLV
EKLFGFLGSL LTTCSSDIPL LRESTLRRRR ARPQASLTAT HSSTLAEEVV ALLRTLHSLT
QWNGLINKYI NSQLRSISHS SVGKLSEGAQ LEDYSPVPEN PEVGGLMAVL AVIGGIDGRL
RLGGQVVHDE FGEGTVTRIT PRGRITVQFS DLRMCRVCPL NQLRPLPAMA FSVTNLPFTE
PMLSVWAQLV NLAGSKLEKH KIKKSPKQAF AGQVDLHLLR SQQLKLYILK AGRALLSHQD
KLRQILSQPA VQETGTVHTD DGAAASPDLG DMSPEGPQPP MILLQQLLAA ATQPSPVKAI
FDKQELEAAA LAVCQCLAVE STHPLSPGFE DCSSSEATTP VSMQHTRLAR VKRRKQSPVP
ALPVVVQLME MGFPRRNIEF ALKSLSGASG NASSLPGVEA LVGWLLDHSD VQITELSDAE
TASDEYSDEE VAESVEGAAY SLSTGAVVTE SQTYKKRADF LSNDDYAVYV RENIQVGMTV
RCCRTYEEVC EGDVGKVIKL DRDGLHDLNV QCDWQQKGGT YWVRYIHVEL IGYPPPSSSS
HIKIGDKVRV KASVTTPKYK WGSVTHQSVG IVKAFSANGK DIIVDFPQQS HWTGLLSEME
LVPSIHPGVT CDGCQMFPIN GSRFKCRNCD DFDFCETCFK TKKHNTRHTF GRINEPGQSA
VFCGRSGKQL KRCHSSLPGM LLDSWARMVK SLNVSSSVNQ ASRLIDGSEP CWQSSGSQGK
HWIRLEIFPD VLVHRLKMIV DPADSSYMPS LVVVSGGNSL NNLIELKTIN INPTDTTVPL
LNDCTEYHRY IEIAIKQCRS SGIDCKIHGL ILLGRIRAEE EDLAAVPFLA SDDEEEEDDK
GSSGSLIRKK SAGLESSAAV RTKVFVWGLN DKDQLGGLKG SKIKVPSFSE TLSALNVVQV
AGGSKSLFAV TVEGKVYACG EATNGRLGLG LSSGTVPIPR QITALSSYVV KKVAVHSGGR
HATALTVDGK VFSWGEGDDG KLGHFSRMNC DKPRLIEALR AKRIRDIACG SSHSAALTSS
GELYTWGLGE YGRLGHGDNT TQLKPKMVKV LLGHRVIQVA CGSRDAQTLA LTDEGLVFSW
GDGDFGKLGR GGSEGCNIPQ NIERLNGQGV CQIECGAQFS LALTKSGVVW TWGKGDYFRL
GHGSDVHVRK PQVVEGLRGK KIVHVAVGAL HCLAVTDSGQ VYAWGDNDHG QQGNGTTTVN
RKPTLVQGLE GQKITRVACG SSHSVAWTTV DVATPSVHEP VLFQTARDPL GASYLGVSSD
ADSSAASNKI SGANNSKPSR PSLAKILLSL DGSLAKQQAL SHVLTALQIL YARDAVVGAL
MPAGMIAPVE CPSFSSSAPP SEASAMLSPM TVEDCMLAID LEDRLSPSPW QEKREIVSSE
DAVTPSAVTP SAPSASSRPF IPVTDDPGAA SIIAETMTKT KEDVESQNKA SGPEPQSLDE
FTSLLIVDDT RVVVDLLKLS VCGRAGDKGR EVLSAVLSGM GTAYPQVADM LLELCVTELE
DVATDSQSGR LSSQPVVVES SHPYTDDTST SGTVKIPGAE GLRVEFDRQC STERRHDPLT
VMDGVNRIVS VRSGREWSDW SSELRIPGDE LKWKFISDGS VNGWGWRFTV YPIMPAAGPK
DLLSDRCVLS CPSMDLVTCL LDFRLNLASN RSIVPRLAAS LAACAQLSAL AAGHRMWALQ
RLRKLLTTEF GQSININRLL GENDGETRAL SFTGSALAAL VKGLPEALQR QFEYEDPIVR
GGKQLLHSPF FKVLVALACD LELDALPCCA ETHKWAWFRR YCMASRVAEA LDKRTPSPRL
FLDEVAKKIR ELMADSENMD VLHESHDIFK REQDEQLVQW MNRRPDDWTL SAGGSGTIYG
WGHNHRGQLG GIEGAKVKVP TPCEALATLR PVQLIGGEQT LFAVTADGKL YATGYGAGGR
LGIGGTESVS APTLLESIQH VFIRKVAVNS GGKHCLALSS EGEVYSWGEA EDGKLGHGSR
SPCDRPRVVE SLRGIEVVDV AAGGAHSACV TAAGDLYTWG KGRYGRLGHS DSEDQLKPKL
VEVLQGHRVV DVACGSGDAQ TLCLTDDDTV WSWGDGDYGK LGRGGSDGCK VPMKIESLTG
LGVVKVECGS QFSVALTKSG AVYTWGKGDY HRLGHGSDDH VRRPRQVQGL QGKKVIAIAT
GSLHCVCCTE DGEVYTWGDN DEGQLGDGTT NAIQRPRLVA ALQGKKVNRV ACGSAHTLAW
STSKPSSAGK LPAQVPMEYN HLQEIPIIAL RNRLLLLHHI SELFCPCIPM FDLEGSLDET
GLGPSVGFDT LRGILISQGK EVAFRKVVQA TMVRDRQHGP VVELNRIQVK RSRSKGGLAG
PDGTKSVFGQ MCAKMSSFSP DSLLLPHRVW KVKFVGESVD DCGGGYSESI AEICEELQNG
LTPLLIVTPN GRDESGANRD CYLFNPAAKA LVHSSMFRFL GVLLGIAIRT GSPLSLSLAE
PVWKQLAGMS LTIADLSEVD KDFIPGLMYI RDNEATSEEF EAMSLPFTVP SASGQDIQLS
SKYTHITLDN RAEYVRLAIN YRLHEFDEQV AAVREGMARV VPVPLLSLFT GCELETMVCG
SPDIPLHLLK SVATYKGVEP SASLTQWFWE VMESFSNTER SLFLRFVWGR TRLPRTIADF
RGRDFVIQVL DKYSPPDHFL PESYTCFFLY SCKQVLEEKL KYAIHFCKSI DTDDYARIAL
TGEPAADDSS DDSDNEDVDS FASDSTQDYL TGH
//
