ID G5B2N4_HETGA Unreviewed; 654 AA.
AC G5B2N4;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=GW7_11536 {ECO:0000313|EMBL:EHB03545.1};
OS Heterocephalus glaber (Naked mole rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Bathyergidae;
OC Heterocephalus.
OX NCBI_TaxID=10181 {ECO:0000313|EMBL:EHB03545.1, ECO:0000313|Proteomes:UP000006813};
RN [1] {ECO:0000313|EMBL:EHB03545.1, ECO:0000313|Proteomes:UP000006813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993625; DOI=10.1038/nature10533;
RA Kim E.B., Fang X., Fushan A.A., Huang Z., Lobanov A.V., Han L.,
RA Marino S.M., Sun X., Turanov A.A., Yang P., Yim S.H., Zhao X.,
RA Kasaikina M.V., Stoletzki N., Peng C., Polak P., Xiong Z., Kiezun A.,
RA Zhu Y., Chen Y., Kryukov G.V., Zhang Q., Peshkin L., Yang L., Bronson R.T.,
RA Buffenstein R., Wang B., Han C., Li Q., Chen L., Zhao W., Sunyaev S.R.,
RA Park T.J., Zhang G., Wang J., Gladyshev V.N.;
RT "Genome sequencing reveals insights into physiology and longevity of the
RT naked mole rat.";
RL Nature 479:223-227(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; JH168140; EHB03545.1; -; Genomic_DNA.
DR AlphaFoldDB; G5B2N4; -.
DR STRING; 10181.G5B2N4; -.
DR eggNOG; KOG0496; Eukaryota.
DR InParanoid; G5B2N4; -.
DR Proteomes; UP000006813; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 3.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR PANTHER; PTHR23421:SF53; BETA-GALACTOSIDASE-1-LIKE PROTEIN 2; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000006813}.
FT DOMAIN 54..383
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 443..542
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 566..625
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 201
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 277
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 654 AA; 74050 MW; 56DF18C125AA86BB CRC64;
MSTWSLRRRP GRTLGLLLLV LLSFLVLRRL DWSTLIPPWL RHQRLGLQAK SQNFILEDTT
FWIFGGSIHY FRVPREYWRD RLLKMKACGL NTLTTYVPWN LHEPERGKFD FSGNLDLEAF
VLLAAEVGLW VILRPGPYVC AEIDLGGLPS WLLQDPGMKL RTTYKGFTEA VDLYFDHLMS
RVVPLQYKHG GPIIAVQVEN EYGSYNRDPA YMPYVKKALE DRGIIELLLT SDNKDGLQKG
VVHGVLATIN LQSQQELQLL TTFLLSVQGN QPKMVMEYWT GWFDSWGSPH NILDSSEVLE
TVSAIVNAGS SINLYMFHGG TNFGFINGAM HFNEYKSDVT SYGKQFWGQG RLRQLHGCLA
DYDAVLTEAG DYTAKYGKLR DFFGSRSGAP LPPPPDLLPK MAYEPIAPSF YLSLWDALKY
MEKPIKSEKP INMENLPVND GNGQAFGYTL YETTIASSGV LHGHVRDQGQ VFVNTVSIGF
LDYKTTKIVI PLIQGYTVLR ILVENRGRVN YGNNIDDQRK GLIGNLYLNN SPLKNFRIYS
LDMKKSFFQR FGTDKWSTLP EAPTFPAFFL GVLSVVPSPS DTFLKLEGWE KGVVFINGQN
LGRYWNIGPQ ETLYLPGAWL NPGDNQVIIF EEAMAGPMVQ STDTAHLGRN QYIH
//
