ID G5CK46_KLEPN Unreviewed; 212 AA.
AC G5CK46;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=Alkylmercury lyase {ECO:0000256|ARBA:ARBA00018180, ECO:0000256|HAMAP-Rule:MF_00714};
DE EC=4.99.1.2 {ECO:0000256|ARBA:ARBA00013237, ECO:0000256|HAMAP-Rule:MF_00714};
DE AltName: Full=Organomercurial lyase {ECO:0000256|ARBA:ARBA00031271, ECO:0000256|HAMAP-Rule:MF_00714};
GN Name=merB {ECO:0000256|HAMAP-Rule:MF_00714,
GN ECO:0000313|EMBL:AHG97200.1};
GN ORFNames=BB784_29965 {ECO:0000313|EMBL:APP56115.1};
OS Klebsiella pneumoniae.
OG Plasmid pKp_Goe_024-2 {ECO:0000313|EMBL:APP56115.1},
OG Plasmid pRMH760 {ECO:0000313|EMBL:AHG97200.1}, and
OG Plasmid pkp_goe_024-2 {ECO:0000313|Proteomes:UP000184679}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573 {ECO:0000313|EMBL:AEP25451.1};
RN [1] {ECO:0000313|EMBL:AEP25451.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ND6 {ECO:0000313|EMBL:AEP25451.1};
RX PubMed=22488489; DOI=10.1007/s00284-012-0118-3;
RA Jan A.T., Azam M., Ali A., Haq Q.M.;
RT "Molecular characterization of mercury resistant bacteria inhabiting
RT polluted water bodies of different geographical locations in India.";
RL Curr. Microbiol. 65:14-21(2012).
RN [2] {ECO:0000313|EMBL:AHG97200.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pRMH760 {ECO:0000313|EMBL:AHG97200.1};
RX PubMed=24841670; DOI=10.1089/mdr.2014.0012;
RA Harmer C.J., Hall R.M.;
RT "pRMH760, a Precursor of A/C2 Plasmids Carrying blaCMY and blaNDM Genes.";
RL Microb. Drug Resist. 20:416-423(2014).
RN [3] {ECO:0000313|EMBL:APP56115.1, ECO:0000313|Proteomes:UP000184679}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kp_Goe_827024 {ECO:0000313|EMBL:APP56115.1,
RC ECO:0000313|Proteomes:UP000184679};
RC PLASMID=pKp_Goe_024-2 {ECO:0000313|EMBL:APP56115.1}, and pkp_goe_024-2
RC {ECO:0000313|Proteomes:UP000184679};
RA Bohne W., Bunk B., Overmann J., Gross U., Zautner A.E.;
RT "A nosocomial outbreak of oxa48-expressing Klebsialla pneumoniae in Lower
RT Saxony.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC detoxified by the mercuric reductase. {ECO:0000256|ARBA:ARBA00025326,
CC ECO:0000256|HAMAP-Rule:MF_00714}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000165, ECO:0000256|HAMAP-
CC Rule:MF_00714};
CC -!- SIMILARITY: Belongs to the MerB family. {ECO:0000256|ARBA:ARBA00009443,
CC ECO:0000256|HAMAP-Rule:MF_00714}.
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DR EMBL; JN188357; AEP25451.1; -; Genomic_DNA.
DR EMBL; KF976462; AHG97200.1; -; Genomic_DNA.
DR EMBL; CP018704; APP56115.1; -; Genomic_DNA.
DR RefSeq; WP_000761850.1; NZ_WULZ01000049.1.
DR RefSeq; YP_009022285.1; NC_023898.1.
DR SMR; G5CK46; -.
DR GeneID; 75186173; -.
DR PATRIC; fig|573.1476.peg.179; -.
DR Proteomes; UP000184679; Plasmid pkp_goe_024-2.
DR GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.450.410; -; 1.
DR HAMAP; MF_00714; MerB; 1.
DR InterPro; IPR004927; MerB.
DR InterPro; IPR024259; MerB_HTH_dom.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF12324; HTH_15; 1.
DR Pfam; PF03243; MerB; 1.
DR PIRSF; PIRSF001458; MerB; 1.
DR PRINTS; PR01699; ORGNOHGLYASE.
DR SUPFAM; SSF160387; NosL/MerB-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00714};
KW Mercuric resistance {ECO:0000256|ARBA:ARBA00022466, ECO:0000256|HAMAP-
KW Rule:MF_00714};
KW Mercury {ECO:0000256|ARBA:ARBA00022914, ECO:0000256|HAMAP-Rule:MF_00714};
KW Plasmid {ECO:0000313|EMBL:AHG97200.1}.
FT DOMAIN 8..75
FT /note="Alkylmercury lyase helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12324"
SQ SEQUENCE 212 AA; 23078 MW; 165E6640E11950E2 CRC64;
MKLAPYILER LTSVNRTNGT ADLLVPLLRE LAKGRPVSRT TLAGILDWPA ERVAAVLEQA
TSTEYDKDGN IIGYGLTLRE TSYVFEIDDR RLYAWCALDT LIFPALIGRT ARVSSHCAAT
GAPVSLTVSP SEIQAVEPAG MAVSLVLPQE AADVRQSFCC HVHFFASVPT AEDWASKHQG
LEGLAIVSVH EAFGLGQEFN RHLLQTMSSR TP
//
