ID G5F1V8_9ACTN Unreviewed; 471 AA.
AC G5F1V8;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 13-SEP-2023, entry version 49.
DE RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
GN ORFNames=HMPREF1008_00352 {ECO:0000313|EMBL:EHF02707.1};
OS Olsenella sp. oral taxon 809 str. F0356.
OC Bacteria; Actinomycetota; Coriobacteriia; Coriobacteriales; Atopobiaceae;
OC Olsenella.
OX NCBI_TaxID=661087 {ECO:0000313|EMBL:EHF02707.1, ECO:0000313|Proteomes:UP000003446};
RN [1] {ECO:0000313|EMBL:EHF02707.1, ECO:0000313|Proteomes:UP000003446}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0356 {ECO:0000313|EMBL:EHF02707.1,
RC ECO:0000313|Proteomes:UP000003446};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Izard J., Blanton J.M.,
RA Baranova O.V., Tanner A.C., Dewhirst F.E., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Alvarado L., Arachchi H.M.,
RA Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C., Freedman E.,
RA Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D.,
RA Howarth C., Larson L., Lui A., MacDonald P.J.P., Montmayeur A., Murphy C.,
RA Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Olsenella sp. oral taxon 809 strain F0356.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. Sucrose
CC phosphorylase subfamily. {ECO:0000256|ARBA:ARBA00008452}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHF02707.1}.
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DR EMBL; ACVE01000002; EHF02707.1; -; Genomic_DNA.
DR RefSeq; WP_009278142.1; NZ_JH376563.1.
DR AlphaFoldDB; G5F1V8; -.
DR STRING; 661087.HMPREF1008_00352; -.
DR PATRIC; fig|661087.3.peg.351; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_021358_1_0_11; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000003446; Unassembled WGS sequence.
DR GO; GO:0004645; F:1,4-alpha-oligoglucan phosphorylase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11355; AmyAc_Sucrose_phosphorylase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR InterPro; IPR016377; Sucrose_GGa_phosphorylase-rel.
DR InterPro; IPR022527; Sucrose_phospho.
DR NCBIfam; TIGR03852; sucrose_gtfA; 1.
DR PANTHER; PTHR38784; SUCROSE PHOSPHORYLASE; 1.
DR PANTHER; PTHR38784:SF1; SUCROSE PHOSPHORYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF003059; Sucrose_phosphorylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000003446};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..405
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 193
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT ACT_SITE 234
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT BINDING 49
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 87
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 191..193
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 234
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 291..292
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 335..338
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 392
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
SQ SEQUENCE 471 AA; 54087 MW; 252EDF996F4589EE CRC64;
MRFENKIMLI TYADSLGKNL RDLDQVLARH FDKAVGGVHI LPFFPSSADR GFAPMRYDIV
DPALGSWDDV RRIGKRRYLM FDFMINHISR QSPYFQDFLA KKDESEFADF FIRYKDFWPG
GAPTQEQVDA IYKRKPKAPS VVAEFADGSR EDVWCTFGEE QIDINIRSDR AQRFFRETLT
HMAENNASII RLDAFAYAAK RAGSSCFFVE PDTTELLRDM QEVLDPYDVM VLPEIHEHYT
IQMQVADKGY WVYDFALPVL TLHALYSGEG SYLANWLIMS PKHQFTTLDT HDGLGIVDVK
DLLPDEAVDY TKDKMFSEGA NVKKVYNTEA YNNLDIYQVN TTYYSALGND DDAYLLARAV
QFFARGVPMV YYVGLLAGSN DIELLESTKE GRNINRHYYS VDEIDREVER PVVRDLVELM
ELRNSHPAFD VEGELEADAQ GSRLRIRRTA GEAWAQLDAD LSSHSFEVTH S
//
