ID G5J950_CROWT Unreviewed; 497 AA.
AC G5J950;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase {ECO:0000256|ARBA:ARBA00012034};
DE EC=2.1.1.14 {ECO:0000256|ARBA:ARBA00012034};
DE Flags: Fragment;
GN ORFNames=CWATWH0003_3967a5 {ECO:0000313|EMBL:EHJ11258.1};
OS Crocosphaera watsonii WH 0003.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Aphanothecaceae; Crocosphaera.
OX NCBI_TaxID=423471 {ECO:0000313|EMBL:EHJ11258.1, ECO:0000313|Proteomes:UP000003477};
RN [1] {ECO:0000313|EMBL:EHJ11258.1, ECO:0000313|Proteomes:UP000003477}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WH 0003 {ECO:0000313|EMBL:EHJ11258.1,
RC ECO:0000313|Proteomes:UP000003477};
RX PubMed=22232617;
RA Bench S.R., Ilikchyan I.N., Tripp H.J., Zehr J.P.;
RT "Two Strains of Crocosphaera watsonii with Highly Conserved Genomes are
RT Distinguished by Strain-Specific Features.";
RL Front. Microbiol. 2:261-261(2011).
CC -!- FUNCTION: Catalyzes the transfer of a methyl group from 5-
CC methyltetrahydrofolate to homocysteine resulting in methionine
CC formation. {ECO:0000256|ARBA:ARBA00002777}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
CC {ECO:0000256|ARBA:ARBA00004681}.
CC -!- SIMILARITY: Belongs to the vitamin-B12 independent methionine synthase
CC family. {ECO:0000256|ARBA:ARBA00009553}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHJ11258.1}.
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DR EMBL; AESD01000606; EHJ11258.1; -; Genomic_DNA.
DR AlphaFoldDB; G5J950; -.
DR UniPathway; UPA00051; UER00082.
DR Proteomes; UP000003477; Unassembled WGS sequence.
DR GO; GO:0003871; F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd03312; CIMS_N_terminal_like; 1.
DR Gene3D; 3.20.20.210; -; 2.
DR InterPro; IPR013215; Cbl-indep_Met_Synth_N.
DR InterPro; IPR002629; Met_Synth_C/arc.
DR InterPro; IPR038071; UROD/MetE-like_sf.
DR PANTHER; PTHR30519; 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30519:SF0; 5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE; 1.
DR Pfam; PF08267; Meth_synt_1; 1.
DR Pfam; PF01717; Meth_synt_2; 1.
DR SUPFAM; SSF51726; UROD/MetE-like; 2.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Methionine biosynthesis {ECO:0000256|ARBA:ARBA00023167};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EHJ11258.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EHJ11258.1};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 6..333
FT /note="Cobalamin-independent methionine synthase MetE N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08267"
FT DOMAIN 450..496
FT /note="Cobalamin-independent methionine synthase MetE C-
FT terminal/archaeal"
FT /evidence="ECO:0000259|Pfam:PF01717"
FT NON_TER 497
FT /evidence="ECO:0000313|EMBL:EHJ11258.1"
SQ SEQUENCE 497 AA; 55583 MW; EEE669A7C7B052E7 CRC64;
MNSIKTHSLG YPRIGAKREL KRATEAYWKG DLTLEELLAT GKTIRETNWK KQESAGIGLI
PINDFSFYDQ MLDMSCLLGN VPARFKWTGG KTDVDTVFTV ARGTQGGVSL VEEEKDCQTG
KVSTFASEMT KWFDTNYHYI VPEFRSDTTF KLSSDKIFEE FAEAKALGIN GKPVLIGPVT
YLSLGKIQGT NNPDFDPFSL LDNLVDVYEE IIGKLAAAGA EWIQLDEPIF STDLSKEQLN
ALKKSYDRLQ AAKGTAKILV TTYFGGVREN LGSYLSLPVD ALHFDFVRGA EDVETVLANF
PSDKILSVGI VEGRNIWKNN YDRSLEVLQK ATAAVGADRL WVAPSCSLLH SPVTLENERK
IDEELKSWMA FANEKLQEVV ELRQLLDGTG DEKALQANRE AIKARATSQR IHNEAVKTRL
AGVGDRDFHR ASEFTVRQGK QQEKLQLPEF PTTTIGSFPQ TKEIRKARAQ WKKGAMSDAD
YDQFLKTETA KCVTFQD
//
