ID G5QC83_SALMO Unreviewed; 1022 AA.
AC G5QC83;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=Type I restriction enzyme endonuclease subunit {ECO:0000256|RuleBase:RU364115};
DE Short=R protein {ECO:0000256|RuleBase:RU364115};
DE EC=3.1.21.3 {ECO:0000256|RuleBase:RU364115};
GN ORFNames=LTSEMON_6219 {ECO:0000313|EMBL:EHC71745.1};
OS Salmonella enterica subsp. enterica serovar Montevideo str. S5-403.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=913242 {ECO:0000313|EMBL:EHC71745.1, ECO:0000313|Proteomes:UP000003221};
RN [1] {ECO:0000313|EMBL:EHC71745.1, ECO:0000313|Proteomes:UP000003221}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S5-403 {ECO:0000313|EMBL:EHC71745.1,
RC ECO:0000313|Proteomes:UP000003221};
RX PubMed=21859443; DOI=10.1186/1471-2164-12-425;
RA den Bakker H.C., Moreno Switt A.I., Govoni G., Cummings C.A., Ranieri M.L.,
RA Degoricija L., Hoelzer K., Rodriguez-Rivera L.D., Brown S., Bolchacova E.,
RA Furtado M.R., Wiedmann M.;
RT "Genome sequencing reveals diversification of virulence factor content and
RT possible host adaptation in distinct subpopulations of Salmonella
RT enterica.";
RL BMC Genomics 12:425-425(2011).
CC -!- FUNCTION: Subunit R is required for both nuclease and ATPase
CC activities, but not for modification. {ECO:0000256|RuleBase:RU364115}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851,
CC ECO:0000256|RuleBase:RU364115};
CC -!- SUBUNIT: The type I restriction/modification system is composed of
CC three polypeptides R, M and S. {ECO:0000256|RuleBase:RU364115}.
CC -!- SIMILARITY: Belongs to the HsdR family. {ECO:0000256|ARBA:ARBA00008598,
CC ECO:0000256|RuleBase:RU364115}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHC71745.1}.
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DR EMBL; AFCS01001381; EHC71745.1; -; Genomic_DNA.
DR AlphaFoldDB; G5QC83; -.
DR REBASE; 44036; Sen403ORF6218P.
DR PATRIC; fig|913242.3.peg.5416; -.
DR Proteomes; UP000003221; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd18030; DEXHc_RE_I_HsdR; 1.
DR CDD; cd22332; HsdR_N; 1.
DR CDD; cd18800; SF2_C_EcoR124I-like; 1.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR004473; Restrct_endonuc_typeI_HsdR.
DR InterPro; IPR040980; SWI2_SNF2.
DR NCBIfam; TIGR00348; hsdR; 1.
DR PANTHER; PTHR30195:SF15; TYPE I RESTRICTION ENYME HINDI ENDONUCLEASE SUBUNIT; 1.
DR PANTHER; PTHR30195; TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364115};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU364115};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364115};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU364115};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747,
KW ECO:0000256|RuleBase:RU364115}.
FT DOMAIN 254..431
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 1022 AA; 117151 MW; 48F1B7A0BA207970 CRC64;
MTQKRVIQLF TEHLGYHYLG NWHKREDNRN IEPGLVSAWL TKRGVSEVLI ARAIRQLDRA
ASLGEGRILY DANKEVYRLL RYGVKDKEGA GEQNQTVWLV DWENPDANEF AIAEEVTYNG
ENKKRPDLVL YVNGIALGVI ELKRSTVSVS EGIRQNLDNQ QKAFIRNFFT TIQLVMAGND
LEGLRYGVIE TPEKYYLQWK EAAESPHTNM LDRHLAQICN KQRLLELIHD FIVFDAGVKK
TCRHNQYFGV TATKPFIQRN EGGIIWHTQG SGKSLTMVWL AKWIRENITD SRVLIITDRT
ELDEQIEKVF NGVEEDILRT RSGADLVARL NQTKPWLICS LVHKFGRQEE DDKDADTDDF
IEQMKRNIPA DFSPKGKLFV FVDECHRTQS GKLHSAMTSI LPDAQFIGFT GTPLMKKDKK
KSVEVFGPYI HTYKFDEAVN DGVVLDLRYE ARDIDQRVKS QKKVDQWFDA KTSKLSRLGQ
QQLKQKWASM QKLLSSRSRL EQIVADILLD FEQKPRLADG RGNAMLVCSS VYQACKCYEM
FSDAGFAGKC AIVTSYQPNA SEIKGEESGE GLTEKLAKYN IYRKMLAEYF EQPEAEAAKR
VSDFEKEVKQ RFIKEPGQMR LLIVVDKLLT GFDAPSATYL YIDKKMADHN LFQAICRVNR
LDGEDKEYGY IVDYKDLFRS LEKTVQDYTA EAFDGYDKED VAGLLKDRLQ QAKIDLDGAL
DAVRTLCEPV KMPRNQLDYQ HYFCGESGAS IEQLSEKEAL RLTLYQSVAK LIRAFTNLAG
ELTDAGYSEQ QANQIRIDVE FYTKVRDEIK IASGDFVDMK QFEPGMRQML DLWVDADPSE
TLMDFEELGL LELIIERGEE ALDGLPADMR GNQEAMAEAI ENNVRRTIVD ENPVNPKYYE
KMSVLLDELI ALRRQQAISY QDYLERVREL AKQVKHPQSG SKSTYPASID TLAKKALYDN
LGQDEVLVIK IDTAVRHTKK ADWYGDRFKE REISFAIAEE IKGYSVTVAD VMALVKVQKE
YR
//
