ID G7MI51_MACMU Unreviewed; 1533 AA.
AC G7MI51;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=EGK_01003 {ECO:0000313|EMBL:EHH14980.1};
OS Macaca mulatta (Rhesus macaque).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9544 {ECO:0000313|EMBL:EHH14980.1};
RN [1] {ECO:0000313|EMBL:EHH14980.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CR-5 {ECO:0000313|EMBL:EHH14980.1};
RX PubMed=22002653; DOI=10.1038/nbt.1992;
RA Yan G., Zhang G., Fang X., Zhang Y., Li C., Ling F., Cooper D.N., Li Q.,
RA Li Y., van Gool A.J., Du H., Chen J., Chen R., Zhang P., Huang Z.,
RA Thompson J.R., Meng Y., Bai Y., Wang J., Zhuo M., Wang T., Huang Y.,
RA Wei L., Li J., Wang Z., Hu H., Yang P., Le L., Stenson P.D., Li B., Liu X.,
RA Ball E.V., An N., Huang Q., Zhang Y., Fan W., Zhang X., Li Y., Wang W.,
RA Katze M.G., Su B., Nielsen R., Yang H., Wang J., Wang X., Wang J.;
RT "Genome sequencing and comparison of two nonhuman primate animal models,
RT the cynomolgus and Chinese rhesus macaques.";
RL Nat. Biotechnol. 29:1019-1023(2011).
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR EMBL; CM001253; EHH14980.1; -; Genomic_DNA.
DR Proteomes; UP000013456; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 31..115
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 121..551
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 697..975
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1074..1528
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1533 AA; 174802 MW; 589043FCEF0FCEFB CRC64;
MGHSKQIRIL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVFTNY PFPGETFNRQ
KFRSLDWENP TEREDDSDKY CKLNLQQSGS FQYYFLQGNE KSGGGYIVVD PILRVGADNH
MLPLDCVTLQ TFLAKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SSYSLANQLE
LNPDFSRPNR KYTWNDVGQL VEKLKKEWNI ICITDVVYNH TAANSKWIQE HPECAYNLVN
SPHLKPAWVL DRALWHFSCD VAEGKYKEKG IPALIENDHH MNSIRKMIWE DIFPKLKLWE
FFQVDVNKAV EQFRRLLTQE NRRVTKSDPN QHLKIIQDPE YRRFGCTVDM NIALTTFIPH
DNGPAAIEEC CNWFRQRIEE LNSEKHRLIN YHQEQAVNCL LGNVFYERLA GHGPKLGPVT
RKHPLVTRYF TFPFEEMDFS VEESMIHLPN KACFLMAHNG WVMGDDPLRN FAEPGSEVYL
RRELICWGDS VKLRYGNKPE DCPFLWAHMK KYTEITATYF QGVRLDNCHS TPLHVAEYML
DAARNLQPNL YVVAELFTGS EDLDNIFVTR LGISSLIREA MSAYNSHEEG RLVYRYGGEP
VGSFVQPCLR PLMPAIAHAL FMDITHDNEC PIVHRSAYDA LPSTTIVSMA CCASGSTRGY
DELVPHQISV VSEERFYTKW NPEALPSNTG EVNFQSGIIA ARCAINKLHQ ELGAKGFIQA
VYVDQVDEDI VAVTRHSPSI HQSVVAVSRT AFRNPKTSFY SKEVPQMCIP GKIEEVVLEA
RTIERNTKPY RKDENSINGM PDITVEIREH IQLNESKIVK QAGVATKGPN EYIQEIEFEN
LSPGSVIIFR VSLDPHAQVA VGILRNHLTQ FNPHFKSGSL AVDNSDPILK IPFASIASKL
TLAELNQILY RCESEEKEDG GGCYDIPNWS ALKYAGLQGL MSVLAEIRPK NDLGHPFCNN
LRSGDWMIDY VSNRLISRSG TIAEVGKWLQ AMFFYLKQIP RYLIPCYFDA ILIGAYTTLL
DIAWKQMSSF VQNGSTFVKH LSLGSVQLCG VGKFPSLPIL SPALTDVPYR LNEITKEKEQ
CCVSLAAGLP HFSSGIFRCW GRDTFIALRG ILLITGRYVE ARNIILAFAG TLRHGLIPNL
LGEGTYARYN CRDAVWWWLQ CIQDYCKMVP NGLDILKCPV SRMYPTDDSA PLPAGTLDQP
LFEVIQEAMQ RHMQGIQFRE RNAGPKIDRN MKDEGFNVTA GVDEETGFVY GGNRFNCGTW
MDKMGESDRA RNTGIPATPR DGSAVEIVGL SKSAVRWLLE LSKKNIFPYH EVTVKRHGKV
VKVSYDEWNR KIQDNFEKLF HVSEDPSDLN EKHPNLVHKR GIYKDSYGAS SPWCDYQLRP
NFTIAMVVAP ELFTTAKAWK ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL
AKGFNYHQGP EWLWPIGYFL RAKLYFSRLM GPETTAKTIV LVKNVLSRHY VHLERSPWKG
LPELTNENAQ YCPFSCETQA WSISTILETL YDL
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