ID G7Z320_AZOL4 Unreviewed; 507 AA.
AC G7Z320;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=imuB {ECO:0000313|EMBL:CBS87835.1};
GN OrderedLocusNames=AZOLI_2642 {ECO:0000313|EMBL:CBS87835.1};
OS Azospirillum lipoferum (strain 4B).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=862719 {ECO:0000313|EMBL:CBS87835.1, ECO:0000313|Proteomes:UP000005667};
RN [1] {ECO:0000313|Proteomes:UP000005667}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4B {ECO:0000313|Proteomes:UP000005667};
RX PubMed=22216014; DOI=10.1371/journal.pgen.1002430;
RA Wisniewski-Dye F., Borziak K., Khalsa-Moyers G., Alexandre G.,
RA Sukharnikov L.O., Wuichet K., Hurst G.B., McDonald W.H., Robertson J.S.,
RA Barbe V., Calteau A., Rouy Z., Mangenot S., Prigent-Combaret C.,
RA Normand P., Boyer M., Siguier P., Dessaux Y., Elmerich C., Condemine G.,
RA Krishnen G., Kennedy I., Paterson A.H., Gonzalez V., Mavingui P.,
RA Zhulin I.B.;
RT "Azospirillum genomes reveal transition of bacteria from aquatic to
RT terrestrial environments.";
RL PLoS Genet. 7:E1002430-E1002430(2011).
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
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DR EMBL; FQ311868; CBS87835.1; -; Genomic_DNA.
DR AlphaFoldDB; G7Z320; -.
DR STRING; 862719.AZOLI_2642; -.
DR KEGG; ali:AZOLI_2642; -.
DR HOGENOM; CLU_028184_1_1_5; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000005667; Chromosome.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 3: Inferred from homology;
FT DOMAIN 27..156
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 246..338
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
FT REGION 288..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 507 AA; 55696 MW; 3F78F46933A77884 CRC64;
MGHWGGRRRI LALWLPRLPT DARERTMPPG SRARPFALLR TERQRRMVVA VNRVAASVGI
SPGHTLSDAR ALEPALEVAE ATPETDAILL GRIADWARRY SPWTAADEPD GVVIDITGCA
HLFGGEPALA ADLTGRLRAA GFSARAAIAG TPAAAWGLVR FGSPEDRRQE RLDGLPLAAL
RLPAETVEGL HALGLRRIGD LHAMPRAGLA ARFGARLLQR YDQAFGRLDE PISPRRPVPE
HRERLTFAEP IATPESIAGA LRHLLGRLCK GLEAAGQGAR RLRLDAHRTD QRVDDEPQSL
TLGTSRPTRD PAALARLAAP LLERIDPGPG LETLVLSATE TGPLIAVQTG IDGEGIDGGL
AELVDRLALR LGERAVLRLV PRESWLPERC VAPLPPVDTL PVPKPWPAGR PRPLRLLTPP
EPIEATAPLP DDPPLQFVWR GLRHRVNRAE GPERIECEWW RPEGSLGLAV RDYYRVEDGA
GRRFWLFRAG LYRPGEWPRW FLHGFLG
//
