ID G8H9D4_9VIRU Unreviewed; 1707 AA.
AC G8H9D4;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE SubName: Full=Putative replicase {ECO:0000313|EMBL:AET36890.1};
GN Name=ORF1 {ECO:0000313|EMBL:AET36890.1};
OS Actinidia virus B.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus.
OX NCBI_TaxID=1112770 {ECO:0000313|EMBL:AET36890.1, ECO:0000313|Proteomes:UP000202932};
RN [1] {ECO:0000313|EMBL:AET36890.1, ECO:0000313|Proteomes:UP000202932}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TP7-93B {ECO:0000313|EMBL:AET36890.1};
RX PubMed=22274622; DOI=10.1007/s00705-011-1219-1;
RA Blouin A.G., Chavan R.R., Pearson M.N., Macdiarmid R.M., Cohen D.;
RT "Detection and characterisation of two novel vitiviruses infecting
RT Actinidia.";
RL Arch. Virol. 157:713-722(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
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DR EMBL; JN427015; AET36890.1; -; Genomic_RNA.
DR RefSeq; YP_004935358.1; NC_016404.1.
DR GeneID; 11378337; -.
DR KEGG; vg:11378337; -.
DR Proteomes; UP000202932; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23245; Betaflexiviridae_RdRp; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000202932};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 66..243
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 645..734
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT DOMAIN 887..1175
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1464..1576
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 1707 AA; 195167 MW; AE957227EBB447E7 CRC64;
MSISVSSQRM AAASLMQNGS TVEIESIKTL KTERLKKCET RSDGLFDYYV SDFLRDYLAK
KGVHTSVHSF QAHAHPFSKM IENHLLYNIM SQYIEKTTLF MSFKGSKIKK LLLNHKSGAD
LKNLKCYNRL VHIKDHLRYT DPHKELDMAH LPELARLSET CETAVIHDEV QYWSLQDFQL
VLGQMNKVKR LMYSIIYPAE IDQGYEHSLF PEAYHFERRG GYFIWLPDGQ SDGAYKQPIN
PWLLSTSKTI DSRGRTWTIG KVTSFASHHL FVASLGSTIT EDEYTYDDYT VIPRGALSSG
RRDYSGMYLR SRYVQAVLLY LMALKKPDSS SAVAKMRQLT NGDENPAESL FMAQIARQLQ
DAKLYDSMGT FNLKEAVWNG FCGALGDSIT YLIDKEQYKV SSMEKFILRC SSARITITRT
FRDYQPRRTK LGPELGTSWT GEEDEELQEI YHVVVKEGDR ERTPYSLKGD EILDNKERRS
DLMIQCARMD LLENTKELPS INEPMVFSPI DGFRSMRRFM IREGPIRGFS GLAIGWENDP
NIRLKEGRIT HEQYENIISG TKQATKKQMG QITKCPCGAE LKRTKVEGGY IRSLIPGWTD
QLQGRKAAFY SRHSEEYSYP GGSHKTRGWP AEIERMRNEL GLGEDFDHCL CQIYEKGAGI
PYHADDETCY KEPSVVTVNL FGEADFKTKC TNELSFRLTD GDVLTMGKGF QSNHKHSVQN
TGPGRVSLTF RNSIISNQAD EDDLSEYEET EAGFDETLVV LEKNVKNLCC LDRIAEHMGV
KREVCASIIH SKMPRAIEEF KEGGMSISTF IHVVKQLDLA CYIQNERGNI QVPGKFRELK
VSATGEHMSA YLGPTATSTL ATALDFNPDV SRLSIEVTQS RAIHLLESFR EGFTGVNLNK
YQKRPLGTDI DNTLIDVYGL FGFAGSGKSY YPQTLLRCCN MKDTLVIVPR KALKADWSEK
VKDGAIVRTF ESAFNGRKGY ENIIIDEVGL LPPGYIDLVH ANFQYDTMLL LGDPLQSEYY
NKGDSLFLEP ISESVFDRLM GKKNYLYKTH RLPSNQKLFD VPSKGERSEN YLKGAEGDAN
YDLIITASRA AKEKRGQKGS TIGESQGLSV RRVKLVIDRD WGLLNDKAVM VALTRARNTL
SVEVDKSMKE HLKVHAKSSI LKMFLRGQMI KRELIMEMMG TDNGDVELIE KETRFADSDD
MEDKLSGDPY LKGLLRLYDD VEMEEEEVPD VSLPEPQKTH LPISTKENEL APSLLRAREH
REARTPAGTT EQIDEMGYKM EPENPMTHKA LYLHHRNSDV ATFFLSVKKR LRFMDREKNH
RRFNKVKGFG KQLFKVLKET YNLRQPDKLP DLDRIEAEFA RKRLNKSKNL IEKHSYRSDP
DWPSHYLKIF LKQQVCTKME KRGVDAKAGQ TIACFCHAVL CKFGPLLRRT EKALRAQLGD
NVLIYSQLNY TDLDKWCKNF VPSMLGTDSD YEAFDRSQDE RILDFEMEVL KFFLWPEEVI
NEYKELKLMM GSSMGSLAVM RFSGEFGTFF FNTICNMAFT CLRYKINKDT PICYAGDDMY
APGHLIISKE HEGTLDQLSL KAKVRVSTEP LFCGWRMSPY GIVKDPNLLL DRWKIAKRGG
NLDQCMVNYS LEACYGYRLG EYLFDINIDI DAQQELVREI IKIKHKLPKG IRKLFSSDPT
ECGSDGEELE FRVRNEGGIE SPTETED
//
