ID   G8SHW3_ACTS5            Unreviewed;       705 AA.
AC   G8SHW3;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   SubName: Full=Glycogen operon protein GlgX {ECO:0000313|EMBL:AEV85579.1};
DE            EC=3.2.1.- {ECO:0000313|EMBL:AEV85579.1};
GN   Name=glgX {ECO:0000313|EMBL:AEV85579.1};
GN   OrderedLocusNames=ACPL_4688 {ECO:0000313|EMBL:AEV85579.1};
OS   Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110).
OC   Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC   Micromonosporaceae; Actinoplanes.
OX   NCBI_TaxID=134676 {ECO:0000313|EMBL:AEV85579.1, ECO:0000313|Proteomes:UP000005440};
RN   [1] {ECO:0000313|Proteomes:UP000005440}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31044 / CBS 674.73 / SE50/110
RC   {ECO:0000313|Proteomes:UP000005440};
RA   Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K.,
RA   Wehmeier U.F., Stoye J., Puehler A.;
RT   "The complete genome sequence of the acarbose producer Actinoplanes sp.
RT   SE50/110.";
RL   Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
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DR   EMBL; CP003170; AEV85579.1; -; Genomic_DNA.
DR   RefSeq; WP_014691650.1; NZ_LT827010.1.
DR   AlphaFoldDB; G8SHW3; -.
DR   STRING; 134676.ACPL_4688; -.
DR   KEGG; ase:ACPL_4688; -.
DR   PATRIC; fig|134676.3.peg.4602; -.
DR   eggNOG; COG1523; Bacteria.
DR   HOGENOM; CLU_011725_1_1_11; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000005440; Chromosome.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000313|EMBL:AEV85579.1};
KW   Hydrolase {ECO:0000313|EMBL:AEV85579.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005440}.
FT   DOMAIN          156..564
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          466..485
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        466..480
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   705 AA;  79317 MW;  F0CF788E5669850F CRC64;
     MVQEIWPGNS YPLGASYDGT GTNFAVFSEV ADGVELCLFD DAGTETRHRL CSVDGFVWHG
     YLPGVAAGTR YGYRVHGPYD PAAGLRCNPA KLLLDPYAKA IDSDIDWDES LFGYRFDDPE
     ARNDLDSGRH MMKAVVVNPY FDWRNDRPPR IPYHQTVIYE AHVKGLTRLH PGVPEHLRGT
     YAGLGHPAVI EHLTRLGVTA VELMPVHQFV QDHHLAERGL SNYWGYNSIG FFAPHNAYAA
     WGQRGEQVQE FRAMVRELHA AGIEVILDVV YNHTAEGNHM GPTLSFRGLD NGAYYRLVDD
     DPRYYMDTTG TGNSLLMRNP HTLQLIMDSL RYWVTEMHVD GFRFDLAATL ARQFHEVDKL
     SAFFDLIQQD PVVSQVKLIA EPWDIGDGGY QVGNFPPAWT EWNGKFRDTV RDFWRGEPAT
     LADFTSRLTG SADLYQDDGR RPVASINFIT AHDGFTLHDL VSYNDKHNEA NGEDNNDGES
     HNRSWNCGVE GPADDPDVVA LRARQQRNLL VTLMLSQGVP MLLAGDEFGR TQGGNNNAYC
     QDNEISWVDW SLAESNASLL TFTRRLIMLQ RRHPVFRRRR FLRGAPVHEA GEALPDIAWF
     TPDGTEMADE HWEAGHARTV TVFLNGDAIT EQGRRGERIV DDSFLLMFNA HHEPLRFTAP
     DPGFGKEWAV EVDTADPAAD HDETLTAAEG VDLPGRSIKV LRRRV
//