ID G8SIM6_ACTS5 Unreviewed; 1203 AA.
AC G8SIM6;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=trcF {ECO:0000313|EMBL:AEV81824.1};
GN Synonyms=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN OrderedLocusNames=ACPL_927 {ECO:0000313|EMBL:AEV81824.1};
OS Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110).
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=134676 {ECO:0000313|EMBL:AEV81824.1, ECO:0000313|Proteomes:UP000005440};
RN [1] {ECO:0000313|Proteomes:UP000005440}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31044 / CBS 674.73 / SE50/110
RC {ECO:0000313|Proteomes:UP000005440};
RA Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K.,
RA Wehmeier U.F., Stoye J., Puehler A.;
RT "The complete genome sequence of the acarbose producer Actinoplanes sp.
RT SE50/110.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; CP003170; AEV81824.1; -; Genomic_DNA.
DR RefSeq; WP_014687897.1; NZ_LT827010.1.
DR AlphaFoldDB; G8SIM6; -.
DR STRING; 134676.ACPL_927; -.
DR KEGG; ase:ACPL_927; -.
DR PATRIC; fig|134676.3.peg.817; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_2_2_11; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000005440; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:AEV81824.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000005440}.
FT DOMAIN 666..827
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 845..1002
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1156..1175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1203 AA; 132065 MW; 7E1FDC1E9B2BD402 CRC64;
MQLAGLIPAA LRDRGLARAR DLARKGFVDS DSMDLTAPVS LRPFVVATVA GPADAGGAQR
PVLAVTATSR EADDLADALG CLIEPGRVAV YPSWETLPHE RLSPRSDTVG RRLAVLRRLA
HPGDQPLQVV VAPVRSLLQP QLKGLGDLEP VELVTGREAE LEGVARRLSD MAYARVDLVT
KRGEFAVRGG ILDVFPPTDE HPSRVEFWGD EVEEIRTFAV ADQRTIEQVE RLWAPPCREL
LLTPAVREKA ALLATRHPEL AEILDRLAEG IPVEGMESLA PALLDGTDSM ELLIDCMPAG
THVLLCDPER IRTRAHDLTR TSDEFLEASW AAAAVGGEAP IDVGAVAFKT LADVRAHAAV
LHQPWWTLSP FGLATADEPA AALPWEDAGT VEVSPDTGDA VALAAQPVPL YHGDTERLAA
DLAGWSAAGW AVALVFEGKG TAQRATELLR DAGLGVTPVD SIATPIADGQ LLVTCGGLNH
GFVDEASRLA VITGNDISGG RGASTKDMRK MPARRRNTID PLELKMGDFV VHEQHGIGRY
IELVQRTVNG ADREYLVIEY AASKRGQPGD RLYVPTDQLD QLSRYVGGEQ PTLHKMGGAD
WQKSKARAKK AVKEIAAQLI QLYAARQASK GHSFGPDTPW QRELEDAFPY TETPDQMAAI
IEVKHDMELQ TPMDRLICGD VGYGKTEIAV RAAFKAVQDG KQVAVLVPTT LLAQQHYNTF
TERMSQFPVQ IRQLSRFQTP KEAALTLENA ADGTADIVIG THRLLSKSTR FKNLGLIIVD
EEQRFGVEHK EQLKALRASV DVLTMSATPI PRTLEMAITG IREMSTIATP PEERHPVLTY
VGAYDDKQVA AAIHRELLRD GQVFYLHNRV ESIDRAARRL RELVPEARVA VAHGQMSEEQ
LEKVMVGFWE KEFDVLVCTT IVESGIDIPN ANTLILERAD LLGLAQLHQI RGRVGRGRER
AYAYFLYPRE KPLTEHAHER LATIAQHTEL GAGMYVAMKD LEIRGAGNLL GGEQSGHIEG
VGFDLYVRMV GEAVSAFKGE RPEEEPEVKI DLPIDAHLPT DYIAVERLRL EMYRKLAEAR
DDARLDEVVA EMTDRYGEPP AAVVNLIAVA RFRQLARAYG LTDVSMQGRH LRFAPLALPD
SKQMRLKRYH PDSVYKPAND QVSVPRPQTR RVGGEPLRDQ DLLQWCAQLL KDVLGDAPAP
ARV
//
