ID G9AYR2_VIBCL Unreviewed; 284 AA.
AC G9AYR2;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 13-SEP-2023, entry version 41.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE Flags: Fragment;
GN Name=rpoA {ECO:0000313|EMBL:AEK35297.1};
OS Vibrio cholerae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=666 {ECO:0000313|EMBL:AEK35297.1};
RN [1] {ECO:0000313|EMBL:AEK35297.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VC107 {ECO:0000313|EMBL:AEK35293.1}, VC225
RC {ECO:0000313|EMBL:AEK35296.1}, and VC869
RC {ECO:0000313|EMBL:AEK35297.1};
RX PubMed=21896336; DOI=10.1016/j.meegid.2011.08.020;
RA Thompson C.C., Freitas F.S., Marin M.A., Fonseca E.L., Okeke I.N.,
RA Vicente A.C.;
RT "Vibrio cholerae O1 lineages driving cholera outbreaks during seventh
RT cholera pandemic in Ghana.";
RL Infect. Genet. Evol. 11:1951-1956(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123}.
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DR EMBL; HM003889; AEK35293.1; -; Genomic_DNA.
DR EMBL; HM003892; AEK35296.1; -; Genomic_DNA.
DR EMBL; HM003893; AEK35297.1; -; Genomic_DNA.
DR AlphaFoldDB; G9AYR2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:AEK35297.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..216
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AEK35297.1"
FT NON_TER 284
FT /evidence="ECO:0000313|EMBL:AEK35297.1"
SQ SEQUENCE 284 AA; 31309 MW; 39B66644ADA028FA CRC64;
STTHAKVTLE PLERGFGHTL GNALRRILLS SMPGCAVTEV EIEGVLHEYS TKEGVQEDIL
EILLNLKGLA VRVAEGKDEV FITLNKSGSG PVVAGDITHD GDVEIVNPEH VICHLTSDNA
AIAMRIKVER GRGYVPASAR IHTEEDERPI GRLLVDATFS PVDKIAYSVE AARVEQRTDL
DKLVIDMETN GTLEPEEAIR RAATILAEQL DAFVDLRDVR VPEEKEEKPE FDPILLRPVD
DLELTVRSAN CLKAEAIHYI GDLVQRTEVE LLKTPNLGKK SLTE
//
