ID GLGA_PSEF5 Reviewed; 522 AA.
AC Q4KCP0;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 2.
DT 27-MAR-2024, entry version 91.
DE RecName: Full=Glycogen synthase {ECO:0000255|HAMAP-Rule:MF_00484};
DE EC=2.4.1.21 {ECO:0000255|HAMAP-Rule:MF_00484};
DE AltName: Full=Starch [bacterial glycogen] synthase {ECO:0000255|HAMAP-Rule:MF_00484};
GN Name=glgA {ECO:0000255|HAMAP-Rule:MF_00484}; OrderedLocusNames=PFL_2887;
OS Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=220664;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX PubMed=15980861; DOI=10.1038/nbt1110;
RA Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA Thomashow L.S., Loper J.E.;
RT "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT Pf-5.";
RL Nat. Biotechnol. 23:873-878(2005).
CC -!- FUNCTION: Synthesizes alpha-1,4-glucan chains using ADP-glucose.
CC {ECO:0000255|HAMAP-Rule:MF_00484}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1->4)-
CC alpha-D-glucosyl](n+1) + ADP + H(+); Xref=Rhea:RHEA:18189, Rhea:RHEA-
CC COMP:9584, Rhea:RHEA-COMP:9587, ChEBI:CHEBI:15378, ChEBI:CHEBI:15444,
CC ChEBI:CHEBI:57498, ChEBI:CHEBI:456216; EC=2.4.1.21;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00484};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00484}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC Bacterial/plant glycogen synthase subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_00484}.
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DR EMBL; CP000076; AAY92159.2; -; Genomic_DNA.
DR RefSeq; WP_011061176.1; NC_004129.6.
DR AlphaFoldDB; Q4KCP0; -.
DR SMR; Q4KCP0; -.
DR STRING; 220664.PFL_2887; -.
DR CAZy; GT5; Glycosyltransferase Family 5.
DR GeneID; 57475928; -.
DR KEGG; pfl:PFL_2887; -.
DR PATRIC; fig|220664.5.peg.2942; -.
DR eggNOG; COG0297; Bacteria.
DR HOGENOM; CLU_009583_18_4_6; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000008540; Chromosome.
DR GO; GO:0033201; F:alpha-1,4-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR GO; GO:0009011; F:starch synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd03791; GT5_Glycogen_synthase_DULL1-like; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR HAMAP; MF_00484; Glycogen_synth; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR011835; GS/SS.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR NCBIfam; TIGR02095; glgA; 1.
DR PANTHER; PTHR45825:SF8; GLYCOGEN SYNTHASE; 1.
DR PANTHER; PTHR45825; GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycogen biosynthesis; Glycosyltransferase; Transferase.
FT CHAIN 1..522
FT /note="Glycogen synthase"
FT /id="PRO_0000230256"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 58
FT /ligand="ADP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:57498"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00484"
SQ SEQUENCE 522 AA; 56410 MW; C621C7A33E8EF9A5 CRC64;
MISAVLDTQG DHPQQQAGDR AAPSVPVPGN KTVLPLARPN PNRKRVLFVT SEIADLVKTG
GLGDVSAALP RAMAHLHDVR VLIPGYPQVL HSENPIHIIG ELGGHAALPA CKIGRMDMPD
GLVIYVLICP ELYEREGTPY GANNGRDWPD NHIRFARLGL AAADIAANLA QIHWCPDLVH
AHDWPAGLAP AYMHWRGQRT PTLFTIHNLA YQGVVSLASC PELGIPEHAL QQEGMEFYGK
LSFLKAGMAY ASHITTVSAT YAQEITTPAF GCGLDGFLAS KTQQGLLSGI PNGIDESWDA
ATDAHLLTPF SIGDWDGKAA NAAHVRQMFG LDASSGPLFA VVSRLVYQKG LDLTEAVAEF
IVQSGGQIAI IGRGEPEEEQ SMRALARRFP GRIGVHIGFN ETDARRMFAG SDFLLMPSRY
EPCGLSQMYA QRFGSLPVAR NTGGLADTIE NGVTGFLFDE STVASYQEAL TRAFKVFAYP
ALLNAMRCRA MAAPFNWCKA VEPYAELYEQ LVAKALGQAA RQ
//
