ID GPTC2_MOUSE Reviewed; 527 AA.
AC Q7TQC7; Q8BNJ9; Q8BPM1; Q8CDH9;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 2.
DT 24-JAN-2024, entry version 127.
DE RecName: Full=G patch domain-containing protein 2;
GN Name=Gpatch2; Synonyms=Gpatc2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Eye, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Enhances the ATPase activity of DHX15 in vitro.
CC {ECO:0000250|UniProtKB:Q9NW75}.
CC -!- SUBUNIT: Interacts with DHX15. {ECO:0000250|UniProtKB:Q9NW75}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q9NW75}.
CC Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9NW75}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7TQC7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TQC7-2; Sequence=VSP_010529;
CC Name=3;
CC IsoId=Q7TQC7-3; Sequence=VSP_010530;
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DR EMBL; AK030026; BAC26744.1; -; mRNA.
DR EMBL; AK053781; BAC35520.1; -; mRNA.
DR EMBL; AK083471; BAC38928.1; -; mRNA.
DR EMBL; BC054810; AAH54810.1; -; mRNA.
DR CCDS; CCDS15605.1; -. [Q7TQC7-1]
DR CCDS; CCDS87941.1; -. [Q7TQC7-3]
DR CCDS; CCDS87942.1; -. [Q7TQC7-2]
DR RefSeq; NP_080643.4; NM_026367.4. [Q7TQC7-1]
DR RefSeq; XP_006497258.1; XM_006497195.2.
DR RefSeq; XP_006497259.1; XM_006497196.2.
DR RefSeq; XP_006497260.1; XM_006497197.1.
DR AlphaFoldDB; Q7TQC7; -.
DR BioGRID; 212429; 3.
DR STRING; 10090.ENSMUSP00000065009; -.
DR iPTMnet; Q7TQC7; -.
DR PhosphoSitePlus; Q7TQC7; -.
DR SwissPalm; Q7TQC7; -.
DR EPD; Q7TQC7; -.
DR MaxQB; Q7TQC7; -.
DR PaxDb; 10090-ENSMUSP00000065009; -.
DR ProteomicsDB; 271011; -. [Q7TQC7-1]
DR ProteomicsDB; 271012; -. [Q7TQC7-2]
DR ProteomicsDB; 271013; -. [Q7TQC7-3]
DR Pumba; Q7TQC7; -.
DR Antibodypedia; 20729; 74 antibodies from 14 providers.
DR Ensembl; ENSMUST00000065573.14; ENSMUSP00000065009.8; ENSMUSG00000039210.17. [Q7TQC7-1]
DR Ensembl; ENSMUST00000110943.9; ENSMUSP00000106568.3; ENSMUSG00000039210.17. [Q7TQC7-3]
DR Ensembl; ENSMUST00000160471.8; ENSMUSP00000124407.2; ENSMUSG00000039210.17. [Q7TQC7-2]
DR GeneID; 67769; -.
DR KEGG; mmu:67769; -.
DR UCSC; uc007dzy.2; mouse. [Q7TQC7-1]
DR UCSC; uc011wyf.1; mouse. [Q7TQC7-3]
DR AGR; MGI:1915019; -.
DR CTD; 55105; -.
DR MGI; MGI:1915019; Gpatch2.
DR VEuPathDB; HostDB:ENSMUSG00000039210; -.
DR eggNOG; KOG0154; Eukaryota.
DR GeneTree; ENSGT00410000025698; -.
DR HOGENOM; CLU_041240_2_0_1; -.
DR InParanoid; Q7TQC7; -.
DR OMA; HSWETGH; -.
DR OrthoDB; 5491578at2759; -.
DR PhylomeDB; Q7TQC7; -.
DR TreeFam; TF331954; -.
DR BioGRID-ORCS; 67769; 0 hits in 77 CRISPR screens.
DR ChiTaRS; Gpatch2; mouse.
DR PRO; PR:Q7TQC7; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q7TQC7; Protein.
DR Bgee; ENSMUSG00000039210; Expressed in embryonic brain and 230 other cell types or tissues.
DR ExpressionAtlas; Q7TQC7; baseline and differential.
DR Genevisible; Q7TQC7; MM.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR InterPro; IPR000467; G_patch_dom.
DR PANTHER; PTHR14195; G PATCH DOMAIN CONTAINING PROTEIN 2; 1.
DR PANTHER; PTHR14195:SF4; G PATCH DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF01585; G-patch; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..527
FT /note="G patch domain-containing protein 2"
FT /id="PRO_0000087567"
FT DOMAIN 466..512
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 35..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..75
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..204
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..255
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 116
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NW75"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NW75"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_010529"
FT VAR_SEQ 388..425
FT /note="DHWFSPGARTEHGQHQLLRDNRAERGHKKSCSLKTASR -> E (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_010530"
FT CONFLICT 251
FT /note="D -> Y (in Ref. 1; BAC26744)"
FT /evidence="ECO:0000305"
FT CONFLICT 367
FT /note="S -> P (in Ref. 2; AAH54810)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 58218 MW; 4F4F29FA56BE06B7 CRC64;
MFGADGRPAI GTAAGKSWHF SRTMEELVHD LVSALEESSE QARGGFAETG EHSRNLSCPL
KRQARKRRGR KRRSYNVHHP WETGHCLSEG SDSSLEEPSK DYREKHSNNK KDRSDSDDQM
LVAKRRPSSN LSSSVRGKRL LWHESDFAVD SLGNRTLRRR RKVKRMAVDL PQDVSSKRTM
TQLPEGCRDQ DMDNDRASQY PEFTRKKVKK RKLKGIRPGP KTQEEGGVLE SEERSQPNKD
RMEYEEQKAS DELRSESDTS SLSSTDAGLF TNDEGRQGDD EQSDWFYEKE SGGACGIAGV
VPWWEKDEPA ELDTNLPDPV FESILSGSFP LMSHPGRGGF QARLSRLHGT PSKNIKKSSG
APPSMLSAPG PGSNKRMVHF SPDAHRHDHW FSPGARTEHG QHQLLRDNRA ERGHKKSCSL
KTASRQTSMH LGSLCTGDIK RRRKAAPLPG PTAAGIVGEN AQPILESNIG NRMLQSMGWT
PGSGLGRDGR GIAEPVQAVQ RPKGLGLGFP LPKSSPTSPA PTSGNPA
//
