ID GSTU1_ORYSJ Reviewed; 231 AA.
AC Q10CE7; B7EUJ2; O65032;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=Probable glutathione S-transferase GSTU1;
DE EC=2.5.1.18;
GN Name=GSTU1; Synonyms=GST1; OrderedLocusNames=Os03g0785900, LOC_Os03g57200;
GN ORFNames=OsJ_012308;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15069639; DOI=10.1007/s00438-004-1006-8;
RA Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.;
RT "Organisation and structural evolution of the rice glutathione S-
RT transferase gene family.";
RL Mol. Genet. Genomics 271:511-521(2004).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE.
RX PubMed=12692133; DOI=10.1074/jbc.m303620200;
RA Dixon D.P., McEwen A.G., Lapthorn A.J., Edwards R.;
RT "Forced evolution of a herbicide detoxifying glutathione transferase.";
RL J. Biol. Chem. 278:23930-23935(2003).
CC -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC exogenous and endogenous hydrophobic electrophiles.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutathione + RX = a halide anion + an S-substituted
CC glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC ChEBI:CHEBI:90779; EC=2.5.1.18;
CC -!- SIMILARITY: Belongs to the GST superfamily. Tau family. {ECO:0000305}.
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DR EMBL; DP000009; ABF99228.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13399.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86728.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ28825.1; -; Genomic_DNA.
DR EMBL; AK103358; BAG96039.1; -; mRNA.
DR RefSeq; XP_015630984.1; XM_015775498.1.
DR PDB; 1OYJ; X-ray; 1.95 A; A/B/C/D=1-231.
DR PDBsum; 1OYJ; -.
DR AlphaFoldDB; Q10CE7; -.
DR SMR; Q10CE7; -.
DR STRING; 39947.Q10CE7; -.
DR PaxDb; 39947-Q10CE7; -.
DR EnsemblPlants; Os03t0785900-01; Os03t0785900-01; Os03g0785900.
DR GeneID; 4334347; -.
DR Gramene; Os03t0785900-01; Os03t0785900-01; Os03g0785900.
DR KEGG; osa:4334347; -.
DR eggNOG; KOG0406; Eukaryota.
DR HOGENOM; CLU_011226_18_2_1; -.
DR InParanoid; Q10CE7; -.
DR OMA; YYSWFEA; -.
DR OrthoDB; 767442at2759; -.
DR EvolutionaryTrace; Q10CE7; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10CE7; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR CDD; cd03185; GST_C_Tau; 1.
DR CDD; cd03058; GST_N_Tau; 1.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR040079; Glutathione_S-Trfase.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR045074; GST_C_Tau.
DR InterPro; IPR045073; Omega/Tau-like.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR11260:SF732; GLUTATHIONE S-TRANSFERASE GSTU1-RELATED; 1.
DR PANTHER; PTHR11260; GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING; 1.
DR Pfam; PF13410; GST_C_2; 1.
DR Pfam; PF13417; GST_N_3; 1.
DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR SUPFAM; SSF52833; Thioredoxin-like; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Transferase.
FT CHAIN 1..231
FT /note="Probable glutathione S-transferase GSTU1"
FT /id="PRO_0000185869"
FT DOMAIN 5..84
FT /note="GST N-terminal"
FT DOMAIN 97..220
FT /note="GST C-terminal"
FT BINDING 15
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000269|PubMed:12692133"
FT BINDING 42
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000269|PubMed:12692133"
FT BINDING 56
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000269|PubMed:12692133"
FT BINDING 68..69
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT STRAND 7..11
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 16..28
FT /evidence="ECO:0007829|PDB:1OYJ"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 44..49
FT /evidence="ECO:0007829|PDB:1OYJ"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:1OYJ"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:1OYJ"
FT STRAND 64..68
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 69..79
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 97..124
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 127..148
FT /evidence="ECO:0007829|PDB:1OYJ"
FT STRAND 152..158
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 164..169
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 170..175
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 176..183
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 187..190
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 192..201
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 205..210
FT /evidence="ECO:0007829|PDB:1OYJ"
FT HELIX 214..229
FT /evidence="ECO:0007829|PDB:1OYJ"
SQ SEQUENCE 231 AA; 25871 MW; 5E0F3E78F542B739 CRC64;
MAEEKELVLL DFWVSPFGQR CRIAMAEKGL EFEYREEDLG NKSDLLLRSN PVHRKIPVLL
HAGRPVSESL VILQYLDDAF PGTPHLLPPA NSGDADAAYA RATARFWADY VDRKLYDCGS
RLWRLKGEPQ AAAGREMAEI LRTLEAELGD REFFGGGGGG RLGFVDVALV PFTAWFYSYE
RCGGFSVEEV APRLAAWARR CGRIDSVVKH LPSPEKVYDF VGVLKKKYGV E
//
