ID GTOMC_ORYSJ Reviewed; 362 AA.
AC Q6ZIK0; A0A0P0VNA3; Q1PBH7;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=Probable tocopherol O-methyltransferase, chloroplastic;
DE EC=2.1.1.95 {ECO:0000250|UniProtKB:Q9ZSK1};
DE AltName: Full=Gamma-tocopherol methyltransferase;
DE AltName: Full=Vitamin E pathway gene 4 protein;
DE Flags: Precursor;
GN Name=VTE4; Synonyms=G-TMT, TMT;
GN OrderedLocusNames=Os02g0701600, LOC_Os02g47310; ORFNames=OJ1111_E07.25;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Zou L., Gao H.;
RT "Molecular cloning of rice gamma-TMT.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Involved in the synthesis of tocopherol (vitamin E).
CC Methylates gamma- and delta-tocopherol to form beta- and alpha-
CC tocopherol, respectively. {ECO:0000250|UniProtKB:Q9ZSK1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=gamma-tocopherol + S-adenosyl-L-methionine = (+)-alpha-
CC tocopherol + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:24012,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:18145, ChEBI:CHEBI:18185,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.95;
CC Evidence={ECO:0000250|UniProtKB:Q9ZSK1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=delta-tocotrienol + S-adenosyl-L-methionine = beta-tocotrienol
CC + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:38091,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33275, ChEBI:CHEBI:33276,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.95;
CC Evidence={ECO:0000250|UniProtKB:Q9ZSK1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=gamma-tocotrienol + S-adenosyl-L-methionine = alpha-
CC tocotrienol + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:38095,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33270, ChEBI:CHEBI:33277,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.95;
CC Evidence={ECO:0000250|UniProtKB:Q9ZSK1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=delta-tocopherol + S-adenosyl-L-methionine = beta-tocopherol +
CC H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:37991,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47771, ChEBI:CHEBI:47772,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.95;
CC Evidence={ECO:0000250|UniProtKB:Q9ZSK1};
CC -!- PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. gTMT family. {ECO:0000255|PROSITE-ProRule:PRU00914}.
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DR EMBL; DQ456878; ABE41796.1; -; mRNA.
DR EMBL; AP003994; BAD07529.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09758.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80471.1; -; Genomic_DNA.
DR EMBL; AK061746; BAG88086.1; -; mRNA.
DR EMBL; AK071763; BAG92679.1; -; mRNA.
DR RefSeq; XP_015623086.1; XM_015767600.1.
DR AlphaFoldDB; Q6ZIK0; -.
DR SMR; Q6ZIK0; -.
DR STRING; 39947.Q6ZIK0; -.
DR PaxDb; 39947-Q6ZIK0; -.
DR EnsemblPlants; Os02t0701600-01; Os02t0701600-01; Os02g0701600.
DR GeneID; 4330437; -.
DR Gramene; Os02t0701600-01; Os02t0701600-01; Os02g0701600.
DR KEGG; osa:4330437; -.
DR eggNOG; KOG1269; Eukaryota.
DR HOGENOM; CLU_039068_0_0_1; -.
DR InParanoid; Q6ZIK0; -.
DR OMA; YCLPYVI; -.
DR OrthoDB; 2283604at2759; -.
DR PlantReactome; R-OSA-1119287; Vitamin E biosynthesis.
DR UniPathway; UPA00160; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6ZIK0; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR GO; GO:0050342; F:tocopherol O-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0010189; P:vitamin E biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR025774; MTs_g-TMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR44068:SF11; GERANYL DIPHOSPHATE 2-C-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR44068; ZGC:194242; 1.
DR Pfam; PF08241; Methyltransf_11; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51581; SAM_GTMT; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Methyltransferase; Plastid; Reference proteome;
KW S-adenosyl-L-methionine; Transferase; Transit peptide.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 56..362
FT /note="Probable tocopherol O-methyltransferase,
FT chloroplastic"
FT /id="PRO_0000409867"
FT REGION 141..150
FT /note="SAM motif I"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00914"
FT REGION 204..212
FT /note="SAM motif II"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00914"
FT REGION 231..240
FT /note="SAM motif III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00914"
SQ SEQUENCE 362 AA; 39671 MW; 93BB87DB0D921308 CRC64;
MAHAAAATGA LAPLHPLLRC TSRHLCASAS PRAGLCLHHH RRRRRSSRRT KLAVRAMAPT
LSSSSTAAAA PPGLKEGIAG LYDESSGVWE SIWGEHMHHG FYDAGEAASM SDHRRAQIRM
IEESLAFAAV PDDAEKKPKS VVDVGCGIGG SSRYLANKYG AQCYGITLSP VQAERGNALA
AEQGLSDKVS FQVGDALEQP FPDGQFDLVW SMESGEHMPD KRQFVSELAR VAAPGARIII
VTWCHRNLEP SEESLKPDEL NLLKRICDAY YLPDWCSPSD YVKIAESLSL EDIRTADWSE
NVAPFWPAVI KSALTWKGLT SLLRSGWKTI RGAMVMPLMI EGYKKGLIKF TIITCRKPET
TQ
//
